Abstract
Derivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol lactate per liter of complex medium containing 10% (w/v) sugar (fermentation times of 48 h for glucose, 120 h for sucrose). Both strains produced 667–700 mmol lactate per liter of mineral salts medium. Yields for metabolized sugar ranged from 88% to 95% in both media.
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Zhou, S., Yomano, L., Shanmugam, K. et al. Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B . Biotechnol Lett 27, 1891–1896 (2005). https://doi.org/10.1007/s10529-005-3899-7
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DOI: https://doi.org/10.1007/s10529-005-3899-7