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Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium

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Abstract

A non-recombinant mutant of Escherichia coli B, strain SZ194, was developed that produces over 1 m d-lactate from glucose (or sucrose) in 72 h using mineral salts medium supplemented with 1 mm betaine in simple anaerobic fermentations. Rates and yields were highest at pH 7.5. Yields approached the theoretical maximum with only trace amounts of co-products. Chiral purity of d-lactate was estimated to be 95%. Specific and volumetric productivities for SZ194 in mineral salts medium (pH 7.5) with betaine were equivalent to those in Luria broth.

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Authors and Affiliations

  1. Department of Biological Sciences, Northern Illinois University, DeKalb, IL, 60115, USA

    S. Zhou

  2. Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32611, USA

    K. T. Shanmugam, L. P. Yomano, T. B. Grabar & L. O. Ingram

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  1. S. Zhou
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  2. K. T. Shanmugam
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  3. L. P. Yomano
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  5. L. O. Ingram
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Correspondence to L. O. Ingram.

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Revisions requested 17 January 2006; Revisions received 7 February 2006

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Zhou, S., Shanmugam, K.T., Yomano, L.P. et al. Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium. Biotechnol Lett 28, 663–670 (2006). https://doi.org/10.1007/s10529-006-0032-5

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  • DOI: https://doi.org/10.1007/s10529-006-0032-5

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