Abstract
Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both l(+)- and d(−)-lactate. Strain TG113 contains only native genes and produced optically pure d(−)-lactate. Strain TG108 contains the ldhL gene from Pediococcus acidilactici and produced only l(+)-lactate. In mineral salts medium containing 1 mM betaine, both strains produced over 115 g (1.3 mol) lactate from 12% (w/v) glucose, >95% theoretical yield.
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Acknowledgements
This research was supported by grants from the U.S. Department of Agriculture (01-35504-10669 and 00-52104-9704), the U.S. Department of Energy (FG02-96ER20222 and FG36-04GO14019), BioEnergy International, LLC, and the Florida Agricultural Experiment Station.
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Grabar, T.B., Zhou, S., Shanmugam, K.T. et al. Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli . Biotechnol Lett 28, 1527–1535 (2006). https://doi.org/10.1007/s10529-006-9122-7
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DOI: https://doi.org/10.1007/s10529-006-9122-7