Skip to main content
Log in

Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Enzymatic hydrolysates of orange peel contain relatively high levels of galacturonic acid and arabinose which are not fermentable to ethanol by yeasts. We observed complete utilization of both sugars during fermentation of peel hydrolysates by the ethanologenic construct of E. coli KO11. The bacterium exhibits a novel pattern of galacturonic acid fermentation producing equimolar amounts of acetate and ethanol accompanied by carbon dioxide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Agricultural Research Service (1962). Chemistry and Technology of Citrus, Citrus Products and Byproducts. Agriculture Handbook No. 98, United States Department of Agriculture, Publ., Washington, D.C., 98 pp.

    Google Scholar 

  • Akao, T., Mizuki, E., Saito, H., Okumura, S., and Murao, S. (1992). Bioresource Technol., 41, 35–39.

    Google Scholar 

  • Barbosa, M.F.S., Beck, M.J., Fein, J.E., Potts, D., and Ingram, L.O. (1992). Appl. Environm. Microbiol., 58, 1382–1384.

    Google Scholar 

  • Barnett, J.A., Payne, R.W., and Yarrow, D. (1990). Yeasts Characteristics and Identification, 2nd Ed., Cambridge University Press, Cambridge, United Kingdom, 1002 pp.

    Google Scholar 

  • Beall, D.S., and Ingram, L.O. (1992). Biotechnol. Lett., 14, 857–862.

    Google Scholar 

  • Burchhardt, G., and Ingram, L.O. (1992). Appl Environm. Microbiol., 58, 1128–1133.

    Google Scholar 

  • Echeverria, E., Burns, J.K., and Wicker, L. (1988). Proc. Fla. State Hort. Soc., 101, 150–154.

    Google Scholar 

  • Fisher, C.H., and Chen, J.C.P. (1982). In: Handbook of Processing and Utilization in Agriculture, I.A. Wolff, ed. vol. 1, part 1, pp. 579–610, Boca Raton, Florida: CRC Press.

    Google Scholar 

  • Florida Citrus Processors Association (1993). Statistical Summary 1991–1992 Season. Florida Citrus Processors Association, Publ., Winter Haven, Florida, 63 pp.

    Google Scholar 

  • Grohmann, K., and Baldwin, E.A. (1992). Biotechnol Lett., 14, 1169–1174.

    Google Scholar 

  • Grohmann, K., Baldwin, E.A., and Buslig, B.S. (1993). Appl. Biochem. Biotechnol., (in press).

  • Guimaraes, W.V., Dudey, G.L., and Ingram, L.O. (1992). Biotechnol. Bioeng., 40, 41–45.

    Google Scholar 

  • Hang, Y.D., and Walter, R.H. (1989). In: Processed Apple Products, I.A. Wolff ed. pp. 365–377, New York: VanNostrand Reinhold Publ.

    Google Scholar 

  • Kesterson, J.W., and Braddock, R.J. (1976). By-products and specialty products of Florida citrus. Bulletin #784, University of Florida, Publ., Gainesville, Florida, 119 pp.

    Google Scholar 

  • Lane, A.G., (1980). J. Chem. Tech. Biotechnol., 30, 345–350.

    Google Scholar 

  • Lane, A.G. (1983). Environ. Technol. Lett., 4, 65–72.

    Google Scholar 

  • von Loesecke, H.W. (1934). Citrus Ind., 15, 8–9, 20–21.

    Google Scholar 

  • Marshall, M.R., Graumlich, T.R., Braddock, R.J., and Messersmith, M. (1985). J. Food Sci., 50, 1211–1212.

    Google Scholar 

  • Mizuki, E., Akao, T., and Saruwatari, T. (1990). Biol. Wastes, 33, 161–168.

    Google Scholar 

  • Murdock, D.I., and Allen, W.E. (1960). Food Technol., 14, 441–445.

    Google Scholar 

  • McNary, R., Wolford, R.W., and Patton, V.D. (1951). Food Technol., 5, 319–323.

    Google Scholar 

  • Nishio, N., Oku, Y., Kawamara, D., and Nagai, S. (1979). Hakko Kogaku Kaishi, 57, 354–359.

    Google Scholar 

  • Nishio, N., and Nagai, S. (1979). European J. Appl. Microbiol. Biotechnol., 6, 371–378.

    Google Scholar 

  • Ohta, K., Beall, D.S., Mejia, J.P., Shanmugam, K.T., and Ingram, L.O. (1991). Appl. Environm. Microbiol., 57, 893–900.

    Google Scholar 

  • Pecina, R., Bonn, G., Burtscher, E., and Bobleter, O. (1984). J. Chromatogr., 287, 245–258.

    Google Scholar 

  • Reisch, M.S. (1993). Chem. & Eng. News, 71, 10–13.

    Google Scholar 

  • Subba, M.S., Soumithri, T.C., and Suryanarayana, R. (1967). J. Food Sci., 32, 225–227.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grohmann, K., Baldwin, E.A., Buslig, B.S. et al. Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli . Biotechnol Lett 16, 281–286 (1994). https://doi.org/10.1007/BF00134626

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00134626

Keywords

Navigation