Abstract
This chapter reviews the literature on somaclonal variation affecting the micropropagation through somatic embryogenesis. The first part covers the following aspects: (i) principle, protocols, and applications of somatic embryogenesis and (ii) epigenetic reprogramming and changes in cell fate that underlie somatic embryogenesis. The second part addresses the problem of somaclonal variation in somatic embryogenesis by first (i) assessing their impact on somatic embryo-derived plant production and (ii) describing the multiple origins of somaclonal variation (chromosomal aberrations, genetic alterations, epigenetic regulations, and transposable elements). The last part focuses on how to manage somaclonal variation in commercial productions of SE-derived plants by covering different aspects: (i) detection of undesirable phenotypes: screening out the variants, (ii) strategies of avoidance and incidence limitation, (iii) generation and exploitation of desirable phenotypes in plant breeding, (iv) beyond the induction of stress-tolerant somaclonal variants: a plant breeder’s perspective.
The original version of this chapter was revised: The incorrect tagging of the given names and the family names of the authors was corrected to H. Etienne, R. Guyot, T. Beulé, J.-C. Breitler, E. Jaligot. The erratum to this chapter is available at DOI 10.1007/978-3-319-33705-0_28
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-33705-0_28
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Abbreviations
- AFLP:
-
Amplified fragment length polymorphism
- 6-BA:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- LTR:
-
Long terminal repeats
- PGRs:
-
Plant growth regulators
- RAPD:
-
Random amplified polymorphic DNA
- SE:
-
Somatic embryogenesis
- SSR:
-
Simple sequence repeat
- SV:
-
Somaclonal variation
- TEI:
-
Transgenerational epigenetic inheritance
- TEs:
-
Transposable elements
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Etienne, H., Guyot, R., Beulé, T., Breitler, JC., Jaligot, E. (2016). Plant Fidelity in Somatic Embryogenesis-Regenerated Plants. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Somatic Embryogenesis: Fundamental Aspects and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-33705-0_8
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