Abstract
Primary globular callus from immature zygotic embryos and friable embryogenic tissue derived from mature zygotic embryos were used to establish suspension cultures. Callus cultures were established either on modified Y3 or MS medium containing 475–500 μM 2,4-D or 250 μM picloram and 0.3% (w/v) activated charcoal. Suspension cultures of both cell lines were established in modified Y3 medium containing 10 μM 2,4-D. The establishment of cell suspensions from friable embryogenic tissue took only 2 months, in contrast with suspensions from primary globular callus which took 3–5 months to establish. Embryo differentiation was observed only in cell suspensions derived from the friable embryogenic tissue after plating aliquots on regeneration medium. Germinated embryos were recovered and plantlets were successfully established under greenhouse conditions.
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Abbreviations
- CET:
-
compact embryogenic tissue
- FET:
-
friable embryogenic tissue
- CIM:
-
callus induction medium
- PGC:
-
primary globular callus
- 2,3-D:
-
2,4-dichlorphenoxyacetic acid Y3-Eeuwens' medium
- MS:
-
Murashige & Skoog medium
- PVP-40:
-
polyvinylpyrrolidone
- KM:
-
Kao & Michayluk vitamins
- ABA:
-
abscisic acid
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Teixeira, J.B., Söndahl, M.R., Nakamura, T. et al. Establishment of oil palm cell suspensions and plant regeneration. Plant Cell Tiss Organ Cult 40, 105–111 (1995). https://doi.org/10.1007/BF00037662
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DOI: https://doi.org/10.1007/BF00037662