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DNA methylation as a key process in regulation of organogenic totipotency and plant neoplastic progression?

  • Cell Biology
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Summary

Progressive loss of organogenic totipotency appears to be a common event in long-term plant tissue culture. This loss of totipotency, which has been proposed to be a typical trait of plant neoplastic progression, is compared to some mechanisms that occur during the establishment of animal differentiation-resistant cancer lines in vitro. Evidence is presented that alteration in DNA methylation patterns and expression of genes occur during long-term callus culture. An effect of the auxin, 2,4-dichlorophenoxyacetic acid, in the progressive methylation, is moreover suggested. Methylation of genes relevant to cell differentiation and progressive elimination of cells capable of differentiation is proposed as being responsible for this progressive loss of organogenic potential. Finally, the epigenetic alteration (DNA methylation) that occurs during prolonged periods of culture may induce other irreversible genetic alterations that ultimately make the loss of totipotency irreversible.

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  1. Laboratoire de Morphologie, Institut de Biologie Végétale B22, Sart Tilman, B-4000, Liège, Belgium

    Pascal Lambé, Hity Schié Nkung Mutambel & Roger Deltour

  2. Laboratoire d’Hormonologie Fondamentale et Appliquée, Institut de Biologie Végétale B22, Sart Tilman, B-4000, Liège, Belgium

    Jean-Gabriel Fouché & Thomas Gaspar

  3. Laboratoire de Biologie Générale, Institut de Pathologie B 23, CHU, Sart Tilman, B-4000, Liège, Belgium

    Jean-Michel Foidart

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Lambé, P., Mutambel, H.S.N., Fouché, JG. et al. DNA methylation as a key process in regulation of organogenic totipotency and plant neoplastic progression?. In Vitro Cell.Dev.Biol.-Plant 33, 155–162 (1997). https://doi.org/10.1007/s11627-997-0015-9

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