Genetic Manipulations with Plant Material

  • Lucien Ledoux

Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Analysis of Microbial Genome Structure

  3. The Mechanism of Competence for DNA Uptake and Transformation in Pneumococci

  4. Integrated and Free State of Plasmids

  5. F-Prime Manipulations of Possible Interest to Plant Biologists

  6. Interallelic Complementation in the Study of Gene Action

    1. R. F. Matagne, R. Loppes
      Pages 77-88
  7. Principles of Genetic Regulation in Lower and Higher Plants

    1. R. Loppes, R. F. Matagne
      Pages 89-105
  8. The Enzymology of Nitrogen Fixation

    1. John Postgate
      Pages 107-122
  9. The Physiology and Genetics of Nitrogen Fixation

  10. Molecular Biology of the Genus Agrobacterium

  11. Crown Gall: A Model for Tumor Research and Genetic Engineering

    1. R. A. Schilperoort, G. H. Bomhoff
      Pages 141-162
  12. The Role of Plasmids in Crown-Gall Formation by a.Tumefaciens

  13. Genetic Mechanisms in Differentiation and Development

  14. Plant Cell Cultures: Present and Projected Applications for Studies in Cell Metabolism

  15. Plant Cell Cultures: Present and Projected Applications for Studies in Genetics

  16. Heterogeneous Associations of Cells Formed in Vitro

    1. P. S. Carlson, R. S. Chaleff
      Pages 245-261
  17. Plant Regeneration and Chromosome Stability in Tissue Cultures

  18. Single Cell Culture of an Haploid Cell: The Microspore

  19. Plant Protoplasts as Genetic Systems

    1. E. C. Cocking
      Pages 311-327
  20. Induction of Auxotrophic Mutations in Plants

  21. In Vitro Selection for Mutants of Higher Plants

    1. R. S. Chaleff, P. S. Carlson
      Pages 351-363
  22. Isozymes and a Strategy for Their Utilization in Plant Genetics I. Isozymes: Genetic and Epigenetic Control

  23. Isozymes and a Strategy for Their Utilisation in Plant Genetics II. Isozymes as a Tool in Plant Genetics

  24. Physico-Chemical Aspects of Chromatin and Chromosome Structure

  25. Isolation and Gradient Analysis of DNA

    1. Pol Charles
      Pages 405-428
  26. Use of Molecular Sieving on Agarose Gels to Study DNA Uptake by Chlamy-Domonas Reinhardi

  27. Molecular Hybridization and Its Application to RNA Tumor Virus Research

  28. DNA-Hybridization Studies of the Fate of Bacterial DNA in Plants

  29. Fate of Exogenous DNA in Plants

    1. L. Ledoux
      Pages 479-498
  30. DNA Mediated Genetic Correction of Thiamineless Arabidopsis Thaliana

    1. L. Ledoux, R. Huart, M. Mergeay, P. Charles, M. Jacobs
      Pages 499-517
  31. Uptake of DNA and Bacteriophage into Pollen and Genetic Manipulation

  32. Theoretical and Comparitive Aspects of Bacteriophage Transfer and Expression in Eukaryotic Cells in Culture

  33. Studies on the Use of Transducing Bacteriophages as Vectors for the Transfer of Foreign Genes to Higher Plants

  34. Communications

    1. W. Walczak, J. Wild, K. Krajewska-Grynkiewicz, T. Klopotowski
      Pages 567-568
    2. James H. Meade
      Pages 569-569
    3. A. Mehta, K. Venkatasubbaiah, R. Shah
      Pages 570-570
    4. Z. R. Sung, J. Smith, E. R. Signer
      Pages 574-574
    5. Maria Gutierrez, S. B. Ku, G. E. Edwards
      Pages 575-575
    6. G. Corduan, C. Spix
      Pages 576-577
    7. Donald Grierson
      Pages 579-580
    8. F. Vedel, F. Quetier, J. M. Grienenberger
      Pages 583-583

About this book


Mankind, throughout history, has strived to improve his food sources. By means of slow and empirical selections, it has been possible to greatly increase both quantity and quality of plant crops. This procedure has brought the most useful cereals to a state of refinement that seems to be difficult to further improve by the same methodology. Indeed, natural sexual mechanisms were always used to cross closely related sexually and genetically compatible organisms; the selection procedure consisted of isolating the most promi­ sing progenies. Obviously, by this way, plants could only share preexisting genetic pools. On the other hand, the last decade has seen drastic modifi­ cations of the experimental plant sciences, with the appearance of new technological possibilities. Because of this profound reshaping of our experimental ap­ proaches, other means can now be realistically envisaged in order to achieve similar or even higher goals. It is, for instance, possible to attempt completing sexual crosses (where both male and female gametes bring together the genetic informations necessary for growth and development of the offspring) by parasexual means by which novel genetic informa­ tions could possibly be added to the heritage •. At the limit, such genetic manipulations could enable man to create plants capable of producing new substances characteris­ tic of unrelated plants or, more generally, of other living or­ ganisms. Even if these possibilities might appear quite remote, the interest of Scientists has been awaken and, indeed, several at­ tempts to such genetic manipulations have already been made.


DNA chromosome development genes genetics growth hand hybridization methodology mutagen quality

Editors and affiliations

  • Lucien Ledoux
    • 1
  1. 1.Molecular Genetics, Department of BotanyUniversity of LiègeLiègeBelgium

Bibliographic information