Plant Cell Cultures: Present and Projected Applications for Studies in Genetics

  • H. E. Street
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


This lecture falls into three main sections: (i) Plant tissue and cell cultures offer the possibility of regenerating plants from single cells (including cells regenerated from single or fused protoplasts — Cocking and Evans, 1973). The central problems associated with plant regeneration from callus and suspension cultures will therefore be outlined. (ii) The cytological stability of cells in culture is of critical importance whether our purpose is the use of tissue cultures for multiplication of a particular individual plant phenotype or to preserve a particular cell line or to reveal the phenotypic expression in the whole plant of such a cell line. It is also important if we wish to preserve the haploid states of cells unchanged during a programme of mutagenesis, mutant selection and multiplication. It is therefore necessary for us to be aware of the present state of knowledge regarding cytological instability and to assess how far it may be possible to stabilise the cytology of cells in culture or to circumvent this problem. (iii) The availability of haploid cells via anther and pollen culture (Sunderland, 1973a and Nitsch in this volume) requires attention to the potentiality of such cells and to the problems associated with their use as a source of naturally occurring and induced mutant cell lines. Here it is necessary to consider the use of mutagenic agents and the problems of selecting higher plant cell mutants.


Suspension Culture Plant Cell Culture Haploid Cell Plate Efficiency Carrot Cell 
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  1. Arya, H. C., Hildebrandt, A.C. and Riker, A.J. Pl. Physiol., Lancaster 37, 387–97. 1962.CrossRefGoogle Scholar
  2. Bayliss, M.W. Nature 246, 529–30. 1973.CrossRefGoogle Scholar
  3. Bayliss, M.W. and Gould, A.R. J. exp. Bot., 1974 (in press). Bennici, A., Buiatti, A., Tognoni, F., Rosellini, D. and Giorgi, L. Plant and Cell Physiol. 13, 1–6. 1972.Google Scholar
  4. Bergmann, L. J. gen. Physiol. 43, 841–51. 1960.PubMedCrossRefGoogle Scholar
  5. Binding, H., Binding, K. and Straub, J. Naturwissenschaften 57, 138–9, 1970.PubMedCrossRefGoogle Scholar
  6. Carlson, P.S. Science, N.Y. 168, 487–9. 1970.PubMedCrossRefGoogle Scholar
  7. Carlson, P.S. Science, N.Y. 180, 1366–7. 1973.PubMedCrossRefGoogle Scholar
  8. Cocking, E.C. and Evans, P.K. The isolation of protoplasts, pp. 100–120, in Plant Tissue and Cell Culture. Ed. H.E. Street, Blackwell Scientific Publ. Oxford, 1973.Google Scholar
  9. Dix, P.J. and Street, H.E. Plant Sci. Letters, 1974 (in press). Fox, J.E. PhysiologiaPI. 16, 793–803. 1963.Google Scholar
  10. Gupta, N. and Carlson, P.S. Nature (New Biol.) 239, 86. 1972.Google Scholar
  11. Heimer, Y.M. and Filner, P. Biochim. biophys. Acta 215, 152–65. 1970.Google Scholar
  12. Konar, R.N., Thomas, E. and Street, H.E. Ann. Bot. 36, 123–45. 1972.Google Scholar
  13. Lescure, A.M. Soc. bot. Fr. Memoires 353–65. 1970.Google Scholar
  14. Lescure, A.M. Plant Sci. Letters 1, 375–83. 1973.CrossRefGoogle Scholar
  15. Limasset, P. and Gautheret, R. C.r. hebd. Séanc. Acad. Sci. Paris 230, 2043–5. 1950.Google Scholar
  16. McWilliam, A.A., Smith, S.M. and Street, H.E. Ann. Bot. 38, 243, 1974Google Scholar
  17. Maliga, P., Sz-Breznovits, A. and Marton, L. Nature (New Biol.) 244, 29–30. 1973.Google Scholar
  18. Maliga, P., Marton, L. and Sz-Breznovits, A. Plant Sci. Letters 1, 119–21. 1973.CrossRefGoogle Scholar
  19. Murashige, T. and Skoog, F. Physiologia Pl. 15, 473–97. 1962.CrossRefGoogle Scholar
  20. Nag, K.K. and Street, H.E. Nature 245, 270–2. 1973.CrossRefGoogle Scholar
  21. Nakata, K. and Tanaka, M. Jap. J. Breeding 20, Suppl. 1, 7–8. 1970.Google Scholar
  22. Puck, T. and Kao, F. Proc. Natl. Acad. Sci. U.S.A. 58, 1227. 1967.CrossRefGoogle Scholar
  23. Rashid, A. and Street, H.E. Plant Sci. Letters 2, 89–94. 1974.CrossRefGoogle Scholar
  24. Reinert, J. Aspects of organisation–organogenesis and embryogenesis, pp. 338–355, in Plant Tissue and Cell Culture. Ed. H.E. Street, Blackwell Scientific Publ. Oxford, 1973.Google Scholar
  25. Sievert, R.C. and Hildebrandt, A.C. Am. J. Bot. 52, 742–50. 1965.CrossRefGoogle Scholar
  26. Sievert, R.C., Hildebrandt, A.C., Burns, R.H. and Riberg, A.J. Pl. Physiol., Lancaster. 36, Suppl. X XIX. 1961.Google Scholar
  27. Skoog, F. and Miller, C.O. Symp. Soc. exp. Biol. 11, 118–30. 1957.Google Scholar
  28. Smith, S.M. and Street, H.E. Ann. Bot. 38, 223–241, 1974.Google Scholar
  29. Steward, F.C., Mapes, M.O. and Mears, K. Am. J. Bot. 45, 705–8, 1958.CrossRefGoogle Scholar
  30. Street, H.E. Single-cell clones, pp. 191–204, in Plant Tissue and Cell Culture, Ed. H.E. Street, Blackwell Scientific Publ. Oxford, 1973.Google Scholar
  31. Street, H.E. in Textbook of Developmental Biology. Ed. C.F. Graham and P.F. Wareing. Blackwell Scientific Publ. Oxford, 1974. (in press)Google Scholar
  32. Stuart, R. and Street, H.E. J. exp. Bot. 20, 556–71. 1969. Stuart, R. and Street, H.E. J. exp. Bot. 22, 96–106. 1971.CrossRefGoogle Scholar
  33. Sunderland, N. (a) Pollen and anther culture, pp. 205–239; (b) Nuclear cytology, pp. 161–190, in Plant Tissue and Cell Culture. Ed.Google Scholar
  34. H.E. Street, Blackwell Scientific Publ. Oxford, 1973.Google Scholar
  35. Syono, K. and Furuya, T. Plantand Cell Physiol. 15, 7–17. 1974.Google Scholar
  36. Thomas, E., Konar, R.N. and Street, H.E. J. Cell Science 11, 95–109. 1972.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • H. E. Street
    • 1
  1. 1.Botanical LaboratoriesUniversity of LeicesterLeicesterUK

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