Heterogeneous Associations of Cells Formed in Vitro

  • P. S. Carlson
  • R. S. Chaleff
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


Two experimental systems are described which explore the applicability of in vitro culture to force associations between cells of widely divergent origins. In one experiment, chimeral plants were induced to differentiate from mixed calluses formed by coculturing cells of two tobacco species. In another experiment, a symbiosis was forced between cells of carrot and of the free-living nitrogen-fixing bacterium, Azotobacter vinelandii.


Carrot Cell Graft Union Combine Nitrogen Azotobacter Vinelandii Chimeral Plant 
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  1. Avery, A. G., S. Satina, and J. Rietsema, (1959), Blakeslee: The Genus Datura ( Ronald Press Co., New York).Google Scholar
  2. Bond, G., (1968), Some biological aspects of nitrogen fixation, in “Recent Aspects of Nitrogen Metabolism in Plants”, eds. E. J. Hewitt and C. V. Cutting ( Academic Press, New York), p.Google Scholar
  3. Burris, R. H., (1972), Nitrogen fixation-assay methods and techniques, in “Methods in Enzymology”, ed. A. San Pietro, ( Academic Press, New York ), Vol. 24, 415.Google Scholar
  4. Child, J. J. and T. A. LaRue, (1974), A simple technique for the establishment of nitrogenase in soybean callus culture, Plant Physiol., 53, 88.PubMedCrossRefGoogle Scholar
  5. Davey, M. R. and E. C. Cocking, (1972), Uptake of bacteria by isolated higher plant protoplasts, Nature, 239, 455.CrossRefGoogle Scholar
  6. Goodspeed, T. H., (1954), The Genus Nicotiana (Chronica Botanica Co., Waltham, Mass.).Google Scholar
  7. Hardy, R. W. F., R. D. Holsten, E. K. Jackson and R. C. Burns, (1968), The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation, Plant Physiol., 43, 1185.PubMedCrossRefGoogle Scholar
  8. Holsten, R. D., R. C. Burns, R. W. F. Hardy and R. R. Hebert, (1971), Establishment of symbiosis between Rhizobium and plant cells, Nature, 232, 173.PubMedCrossRefGoogle Scholar
  9. Jorgensen, C. A. and M. B. Crane, (1927), Formation and morphology of Solanum chimaeras, J. Genetics, 18, 247.CrossRefGoogle Scholar
  10. Kao, K. N. and M. R. Michayluk, (1974), A method of high frequency intergeneric fusion of plant protoplasts, Planta, 115, 355.CrossRefGoogle Scholar
  11. Linsmaier, E. M. and F. Skoog, (1965), Organic growth factor requirements of tobacco tissue cultures, Physiol. Plant., 18, 100.Google Scholar
  12. Neilson-Jones, W., (1969), Plant Chimeras (Methuen & Co., Ltd., London).Google Scholar
  13. Oppenheim, J. and L. Marcus, (1970), Correlation of ultrastructure in Azotobacter vinelandii with nitrogen source for growth, J. Bacteriol., 101, 286.PubMedGoogle Scholar
  14. Phillips, D. A., (1974), Factors affecting the reduction of acetylene by Rhizobium-soybean cell associations in vitro, Plant Physiol., 53, 67.PubMedCrossRefGoogle Scholar
  15. Power, J. B., S. E. Cummins and E. C. Cocking, (1970), Fusion of isolated plant protoplasts, Nature, 225, 1016.Google Scholar
  16. Stewart, W. D. P., (1966), Nitrogen Fixation in Plants ( Athlone Press, London).Google Scholar
  17. Vela, G. R., G. D. Cagle and P. R. Holmgren, (1970), Ultrastructure of Azotobacter vinelandii, J. Bacteriol., 104, 933.PubMedGoogle Scholar
  18. Whitehead, T., T. P. McIntosh and W. M. Findlay, (1953), The Potato in Health and Disease ( Oliver and Boyd, Edinburgh).Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • P. S. Carlson
    • 1
  • R. S. Chaleff
    • 1
  1. 1.Biology DepartmentBrookhaven National LaboratoryUptonUSA

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