Plant Protoplasts as Genetic Systems

  • E. C. Cocking
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)


Work on isolated plant protoplasts has progressed very rapidly since the first international meeting held on higher plant protoplasts in Versailles in 19721. Indeed, whereas earlier symposia on protoplasts in general included short sections on those from higher plants2 there are probably now more workers studying higher plant protoplasts than those from micro organisms. As we shall see there has been a major resurgance of interest during the past few years in higher plant protoplasts as genetic systems. At the Versailles Meeting it was evident that some of the foundations for this had been established. Much effort since then has gone into the fuller development of protoplasts as genetic systems, and I will try in this chapter to assess the extent to which this work has been successful and also try to indicate where difficulties still exist.


Somatic Hybridisation Genetic System Leaf Piece Plant Protoplast Crassulacean Acid Metabolism Plant 
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  1. 1.
    Morel, G., Colloques internationaux C.N.R.S. No.212, Protoplastes et Fusion de Cellules Somatiques Végétales (Paris 1973 ).Google Scholar
  2. 2.
    Cocking, E.C., and Pojnar, E., Acta Facultatis Medicae Universitatis Brunensis (Proc. 2nd Int. Symp. Yeast Protoplasts, Brno 1968), 37, 159 (1970).Google Scholar
  3. 3.
    Nickell, L.G., and Heinz, D.J., in Genes, Enzymes and Populations (edit. by Srb, A.M.) 2 (Plenum Press, New York, London, 1973 ), Chapter 9.Google Scholar
  4. 4.
    Evans, P.K., and Cocking, E.C., in New Techniques in Biophysics and Cell Biology (edit. by Pain, R., and Smith, B.) 2 (John Wiley, Chichester, 1974) In the press.Google Scholar
  5. 5.
    Wakasa, K., The Japanese Journal of Genetics, 48, 279 (1973).CrossRefGoogle Scholar
  6. 6.
    Cocking, E.C., and Peberdy, J.F., The Use of Protoplasts from Fungi and Higher Plants as Genetic Systems - A Practical Handbook (Department of Botany, University of Nottingham, 1974).Google Scholar
  7. 7.
    Watts, J.W., and King, J.M., in 64th Annual Report John Innes Institute, p. 78 (Norwich, 1973 ).Google Scholar
  8. 8.
    Scowcroft, W.R., Davey, M.R., and Power, J.B., Plant Science Letters, 1, 451 (1973).CrossRefGoogle Scholar
  9. 9.
    Davey, M.R., Frearson, E.M., Withers, L.A., and Power, J.B., Plant Science Letters, 2, 23 (1974).CrossRefGoogle Scholar
  10. 10.
    Bhojwani, S.S., and Cocking, E.C., Nature New Biology, 239, 29 (1972).PubMedCrossRefGoogle Scholar
  11. 11.
    Cocking, E.C., and Evans, P.K., in Plant Tissue and Cell Culture (edit. Street, H.E.) Chapter 5, Botanical Monographs 11 ( Blackwell, Oxford, 1973 ).Google Scholar
  12. 12.
    Kanai, R., and Edwards, G.E., Plant Physiology, 52, 484 (1973).PubMedCrossRefGoogle Scholar
  13. 13.
    Cocking, E.C., in Dynamic Aspects of Plant Ultrastructure (edit. Robards, A.W.) Chapter 8 (McGraw-Hill, Maidenhead, 1974)Google Scholar
  14. 14.
    Willison, J.H.M., and Cocking, E.C., Protoplasma, in the press.Google Scholar
  15. 15.
    Halperin, W., Canadian Journal of Botany, 51, 1801 (1973).CrossRefGoogle Scholar
  16. 16.
    Wallin, A., and Eriksson, T., Physiologic Plantarum, 28, 33 (1973).CrossRefGoogle Scholar
  17. 17.
    Gurdon, J.B., Nature, 248, 772 (1974).PubMedCrossRefGoogle Scholar
  18. 18.
    Ledoux, L., Uptake of Informative Molecules by Living Cells ( North-Holland Publishing Company, Amsterdam, 1972 )Google Scholar
  19. 19.
    Zaitlin, M., Advances in Virus Research, in the press.Google Scholar
  20. 20.
    Mahlberg, P., American Journal of Botany, 59, 172 (1972).CrossRefGoogle Scholar
  21. 21.
    Willison, J.H.M., Grout, B.W.W., and Cocking, E.C., Bioenergetics, 2, 371 (1971).CrossRefGoogle Scholar
  22. 22.
    Cocking, E.C., and Pojnar, E., Journal of General Virology, 4, 305 (1969).CrossRefGoogle Scholar
  23. 23.
    Burgess, J., Motoyoshi, F., and Fleming, E.N., Planta (Berl.), 111, 199 (1973).CrossRefGoogle Scholar
  24. 24.
    Honda, Y., Matsui, C., Otsuki, Y., and Takebe, I., Phytopathology, 64, 30 (1973).CrossRefGoogle Scholar
  25. 25.
    Hibi, T., and Yora, K., Annals of the Phytopathological Society of Japan, 38, 350 (1972).CrossRefGoogle Scholar
  26. 26.
    Burgess, J., Motoyoshi, F., and Fleming, E.N., Planta (Berl.), 112, 323 (1973).CrossRefGoogle Scholar
  27. 27.
    Takebe, I., and Otsuki, Y., Proceedings of the National Academy of Sciences, USA, 64, 843 (1969).CrossRefGoogle Scholar
  28. 28.
    Grout, B.W.W., and Coutts, R.H.A., Plant Science Letters, 2, 397 (1974).CrossRefGoogle Scholar
  29. 29.
    Pilet, P.E., and Senn, A., C.R. Acad. Sc. Paris, Série D, 278, 269 (1974).Google Scholar
  30. 30.
    Dales, S., Bacteriological Reviews, 37, 103 (1973).Google Scholar
  31. 31.
    Potrykus, I., Zeitschrift für Pflanzenphysiologie, 70, 364 (1973).CrossRefGoogle Scholar
  32. 32.
    Potrykus, I., and Hoffmann, F., Zeitschrift für Pflanzenphysiologie, 69, 287 (1973).CrossRefGoogle Scholar
  33. 33.
    Davey, M.R., Cocking, E.C., and Bush, E., Nature, 244, 460 (1973).CrossRefGoogle Scholar
  34. 34.
    Power, J.B., Cummins, S.E., and Cocking, E.C., Nature, 225, 1016 (1970).PubMedCrossRefGoogle Scholar
  35. 35.
    Kao, K.M., and Michayluk, M.R., Planta (Berl.), 115, 355 (1974).CrossRefGoogle Scholar
  36. 36.
    Keller, W.A., and Melchers, G., Zeitschrift für Naturforschung, 28, 737 (1973).Google Scholar
  37. 37.
    Carlson, P.S., Smith, H.H., and Dearing, R.D., Proceedings of the National Academy of Sciences, USA, 69, 2292 (1972).CrossRefGoogle Scholar
  38. 38.
    Cocking, E.C., in Scienza & Tecnica 74, p. 199 (Arnoldo Mondadorì, Milano, 1974 ).Google Scholar
  39. 39.
    Cocking, E.C., Power, J.B., Evans, P.K., Safwat, F., Frearson,E.M., Hayward, C., Berry, S.F., and George, D., Plant Science Letters, in the press.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • E. C. Cocking
    • 1
  1. 1.Department of BotanyUniversity of NottinghamUniversity Park, NottinghamEngland

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