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Synaptic Vesicles

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Jana Dahlmanns

    1. Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Marc Dahlmanns

    1. Institute for Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2417)

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About this book

This book provides protocols on the preparation of synaptic vesicles from different model systems and provides instructions for functional investigations of their transport and formation, scaffolding, filling and priming, release, recycling, and degradation. Chapters guide readers through computational approaches, simulating different aspects of synaptic vesicle anatomy and function. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  

 

Authoritative and cutting-edge, Synaptic Vesicles: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

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Keywords

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Real-Time Quantitative PCR and Fluorescence In Situ Hybridization for Subcellular Localization of miRNAs in Neurons

    • Antonis Tatarakis, Danesh Moazed
    Pages 1-17

  3. Live-Imaging of Axonal Cargoes in Drosophila Brain Explants Using Confocal Microscopy

    • Caroline Medioni, Anne Ephrussi, Florence Besse
    Pages 19-28

  4. Whole Endosome Recording of Vesicular Neurotransmitter Transporter Currents

    • Roger Chang, Robert H. Edwards
    Pages 29-44

  5. Quantitative Analysis of Presynaptic Vesicle Luminal pH in Cultured Neurons

    • Yoshihiro Egashira, Shutaro Katsurabayashi, Shigeo Takamori
    Pages 45-58

  6. Investigation of Synaptic Vesicle Proteins in Rat Brain Tissue Using Real-Time qPCR

    • Betina Elfving
    Pages 59-68

  7. Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis

    • Chandresh R. Gajera, Rosemary Fernandez, Nadia Postupna, Kathleen S. Montine, C. Dirk Keene, Sean C. Bendall et al.
    Pages 69-88

  8. A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting

    • Heidi K. Müller
    Pages 89-98

  9. Synaptosomes and Metamodulation of Receptors

    • Anna Pittaluga, Mario Marchi
    Pages 99-111

  10. A Novel Method to Monitor GABA Loading into Synaptic Vesicles by Combining Patch Pipette Perfusion and Intracellular, Caged-GABA Photolysis in Brain Slice Preparations

    • Manami Yamashita, Tetsuya Hori
    Pages 113-120

  11. Rapid Isolation of Functional Synaptic Vesicles from Tissues Through Cryogrinding, Ultracentrifugation, and Size Exclusion Chromatography

    • Huinan Li
    Pages 121-130

  12. Isolation of Synaptic Vesicles from Mammalian Brain

    • Marcelo Ganzella, Momchil Ninov, Dietmar Riedel, Reinhard Jahn
    Pages 131-145

  13. Building and Using a Two-Photon Fluorescence Cross-Correlation Spectroscopy Setup Including Fluorescence Lifetime Analysis

    • Tobias Grothe, Peter J. Walla
    Pages 147-166

  14. Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins

    • Tobias Grothe, Peter J. Walla
    Pages 167-180

  15. Synaptic Vesicle Pool Monitoring with Synapto-pHluorin

    • Marc Dahlmanns, Jana Katharina Dahlmanns
    Pages 181-192

  16. Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter

    • Yifu Han, Keke Ding
    Pages 193-203

  17. Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures

    • Céline D. Dürst, Thomas G. Oertner
    Pages 205-219

  18. Dynole 34-2 and Acrylo-Dyn 2-30, Novel Dynamin GTPase Chemical Biology Probes

    • Jennifer R. Baker, Nicholas S. O’Brien, Kate L. Prichard, Phillip J. Robinson, Adam McCluskey, Cecilia C. Russell
    Pages 221-238

  19. Synthesis of Phthaladyn-29 and Naphthalimide-10, GTP Site Directed Dynamin GTPase Inhibitors

    • Cecilia C. Russell, Kate L. Prichard, Nicholas S. O’Brien, Adam McCluskey, Phillip J. Robinson, Jennifer R. Baker
    Pages 239-258

  20. Back Matter

    Pages 259-260
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Editors and Affiliations

  • Department of Psychiatry and Psychotherapy, Universitätsklinikum Erlangen, Erlangen, Germany

    Jana Dahlmanns

  • Institute for Physiology and Pathophysiology, University of Erlangen-Nuremberg, Erlangen, Germany

    Marc Dahlmanns

Bibliographic Information

  • Book Title: Synaptic Vesicles

  • Book Subtitle: Methods and Protocols

  • Editors: Jana Dahlmanns, Marc Dahlmanns

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1916-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1915-5Published: 01 February 2022

  • Softcover ISBN: 978-1-0716-1918-6Published: 02 February 2023

  • eBook ISBN: 978-1-0716-1916-2Published: 31 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 260

  • Number of Illustrations: 28 b/w illustrations, 52 illustrations in colour

  • Topics: Neurosciences

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