Abstract
Despite the important roles of neuropeptides in a variety of physiological processes, there still lacks a method to probe neuropeptide release events in vivo with satisfying temporal and spatial resolution. Neuropeptide Release Reporter (NPRR) was recently introduced as a novel genetically encoded indicator of neuropeptide release with a high temporal resolution and peptide specificity based on GCaMP molecule. Here we describe a method for using NPRR to image selective neuropeptide release at Drosophila neuromuscular junction in semi-dissected larvae. This method provides a quantitative analysis of activity-dependent neuropeptide release as real-time changes in fluorescence intensity of GCaMP reporter with sub-second temporal resolution and single bouton specificity.
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Acknowledgments
Work in the Dickman lab is funded by a grant from the National Institutes of Health (NS091546). We thank Taylor Seid (California Institute of Technology), David Anderson (California Institute of Technology), and Dion Dickman (University of Southern California) for insightful discussions and comments.
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The authors declare no conflicts or other competing interests.
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Han, Y., Ding, K. (2022). Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter. In: Dahlmanns, J., Dahlmanns, M. (eds) Synaptic Vesicles. Methods in Molecular Biology, vol 2417. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1916-2_15
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DOI: https://doi.org/10.1007/978-1-0716-1916-2_15
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