Abstract
A given concentration of GABA can be introduced into a presynaptic terminal by patch clamping the soma of a presynaptic neuron, if the neuron has a relatively short axon. By combining patch pipette perfusion or intracellular, caged-GABA photolysis, it is possible to measure various parameters related to synaptic vesicle filling with GABA.
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Yamashita, M., Hori, T. (2022). A Novel Method to Monitor GABA Loading into Synaptic Vesicles by Combining Patch Pipette Perfusion and Intracellular, Caged-GABA Photolysis in Brain Slice Preparations. In: Dahlmanns, J., Dahlmanns, M. (eds) Synaptic Vesicles. Methods in Molecular Biology, vol 2417. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1916-2_9
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DOI: https://doi.org/10.1007/978-1-0716-1916-2_9
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Publisher Name: Humana, New York, NY
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