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Expanding the repertoire of microsatellite markers for polymorphism studies in Indian accessions of mung bean (Vigna radiata L. Wilczek)

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Abstract

Limited availability of validated, polymorphic microsatellite markers in mung bean (Vigna radiata), an important food legume of India, has been a major hurdle towards its improvement and higher yield. The present study was undertaken in order to develop a new set of microsatellite markers and utilize them for the analysis of genetic diversity within mung bean accessions from India. A GA/CT enriched library was constructed from V. radiata which resulted in 1,250 putative recombinant clones of which 850 were sequenced. SSR motifs were identified and their flanking sequences were utilized to design 328 SSR primer pairs. Of these, 48 SSR markers were employed for assessing genetic diversity among 76 mung bean accessions from various geographical locations in India. Two hundred and thirty four alleles with an average of 4.85 alleles per locus were detected at 48 loci. The polymorphic information content (PIC) per locus varied from 0.1 to 0.88 (average: 0.49 per locus). The observed and expected heterozygosities ranged from 0.40 to 0.95 and 0.40 to 0.81 respectively. Based on Jaccard’s similarity matrix, a dendrogram was constructed using the unweighted pair-group method with arithmetic averages (UPGMA) analysis which revealed that one accession from Bundi, Rajasthan was clustered out separately while remaining accessions were grouped into two major clusters. The markers generated in this study will help in expanding the repertoire of the available SSR markers thereby facilitating analysis of genetic diversity, molecular mapping and ultimately broadening the scope for genetic improvement of this legume.

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Acknowledgments

This research work was supported by the National Institute of Plant Genome Research (NIPGR), New Delhi, India. Financial assistance provided by the Department of Biotechnology (DBT), India through the Project Grant (DBT-PDF Program, IISc, Bangalore) to DS is thankfully acknowledged. We are thankful to Dr. Arjun Lal (NBPGR, New Delhi) and Dr. Rakesh Aggarwal (CAZRI, Jodhpur) for providing the accessions of V. radiata for the study.

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  1. Divya Shrivastava

    Present address: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

Authors and Affiliations

  1. National Institute of Plant Genome Research, Post Box No. 10531, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Divya Shrivastava, Priyanka Verma & Sabhyata Bhatia

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  1. Divya Shrivastava
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  2. Priyanka Verma
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Correspondence to Sabhyata Bhatia.

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11033_2014_3436_MOESM1_ESM.doc

List of 328 V. radiata SSR primer pairs developed in this study. Their sequences, repeat motifs, annealing temperature (Tm), amplification product size (bp) and Genbank accession numbers are mentioned

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Shrivastava, D., Verma, P. & Bhatia, S. Expanding the repertoire of microsatellite markers for polymorphism studies in Indian accessions of mung bean (Vigna radiata L. Wilczek). Mol Biol Rep 41, 5669–5680 (2014). https://doi.org/10.1007/s11033-014-3436-7

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  • DOI: https://doi.org/10.1007/s11033-014-3436-7

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