Summary
Interspecific hybrids have been obtained in an otherwise incompatible cross involving blackgram (Vigna mungo ♀) and greengram (V. radiata ♂). The application of a mixture of gibberellic acid, naphthaleneacetic acid and kintetin to the pollinated buds prevented early abscission of the pod and the abortion of the hybrid embryo. Hybrid embryos (11–17 days after pollination) excised from the young developing pods and cultured on modified Murashige & Skoogs' medium, supplemented with IAA, kinetin, casein hydrolysate or coconut water produced normal-looking plantlets. The younger embryos (less than 11 days after pollination) either proliferated to form callus, or occasionally produced plantlets which albino, spindly and weak. The F1 plants were partially fertile, late-maturing, and intermediate between the two parents with respect to characters such as leaflet size, plant height and number of branches. However, the setting percentage and the number of seeds per pod were highly reduced because of the meiotic irregularities. In the F2 generation, grown in the field as replicated rows, different combinations of desirable characters appeared and the setting percentage was also improved.
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Gosal, S.S., Bajaj, Y.P.S. Interspecific hybridization between Vigna mungo and Vigna radiata through embryo culture. Euphytica 32, 129–137 (1983). https://doi.org/10.1007/BF00036873
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DOI: https://doi.org/10.1007/BF00036873