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Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.)

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Abstract

In order to develop simple sequence repeat (SSR) markers in Italian ryegrass, we constructed a genomic library enriched for (CA)n-containing SSR repeats. A total of 1,544 clones were sequenced, of which 1,044 (67.6%) contained SSR motifs, and 395 unique clones were chosen for primer design. Three hundred and fifty-seven of these clones amplified products of the expected size in both parents of a two-way pseudo-testcross F1 mapping population, and 260 primer pairs detected genetic polymorphism in the F1 population. Genetic loci detected by a total of 218 primer pairs were assigned to locations on seven linkage groups, representing the seven chromosomes of the haploid Italian ryegrass karyotype. The SSR markers covered 887.8 cM of the female map and 795.8 cM of the male map. The average distance between two flanking SSR markers was 3.2 cM. The SSR markers developed in this study will be useful in cultivar discrimination, linkage analysis, and marker-assisted selection of Italian ryegrass and closely related species.

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Acknowledgements

We thank the two anonymous reviewers for their valuable comments on this manuscript. This study was financially supported by a grant from the Japan Racing Association.

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Authors and Affiliations

  1. Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association, 388-5 Higashiakada, Nasushiobara, Tochigi, 329-2742, Japan

    Mariko Hirata, Hongwei Cai, Maiko Inoue, Nana Yuyama & Yuichi Miura

  2. National Institute of Livestock and Grassland Science, 768 Senbonmatsu, Nishinasuno, Tochigi, 329-2747, Japan

    Toshinori Komatsu, Tadashi Takamizo & Masahiro Fujimori

  3. Yamanashi Prefectural Dairy Experiment Station, 621-2 Nagasaka-Kamijo, Nagasaka, Hokuto, Yamanashi, 408-0021, Japan

    Masahiro Fujimori

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  1. Mariko Hirata
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  2. Hongwei Cai
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  3. Maiko Inoue
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  4. Nana Yuyama
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  5. Yuichi Miura
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  6. Toshinori Komatsu
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  7. Tadashi Takamizo
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  8. Masahiro Fujimori
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Correspondence to Mariko Hirata or Masahiro Fujimori.

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Communicated by P. Langridge

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Hirata, M., Cai, H., Inoue, M. et al. Development of simple sequence repeat (SSR) markers and construction of an SSR-based linkage map in Italian ryegrass (Lolium multiflorum Lam.). Theor Appl Genet 113, 270–279 (2006). https://doi.org/10.1007/s00122-006-0292-4

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