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Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers

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Abstract

In this paper, we report the development and characterization of genic microsatellite markers for mungbean by mining sequence database, and transferability of the markers to Asian Vigna species. A total of 157 markers were designed upon searching for SSR in 830 transcript sequences. Thirty-three loci revealed single copy polymorphism in a panel of 17 mungbean accessions with number of alleles ranging from 2 to 8, and observed heterozygosity from 0 to 0.3125. Seven loci deviated from Hardy–Weinberg Equilibrium. Two pairwise combinations of allele frequencies implied linkage disequilibrium. Cross-species amplification in 19 taxa of Asian Vigna using 85 primers showed that amplification rates varied between 80% (V. aconitifolia) to 95.3% (V. reflexo-pilosa). These mungbean genic microsatellite markers will be useful to study genetic resource and conservation of Asian Vigna species.

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References

  • Gwag JK, Chung JW, Chung HK et al (2006) Characterization of new microsatellite markers in mungbean, Vigna radiata (L.). Mol Ecol Notes 6:1132–1134. doi:10.1111/j.1471-8286.2006.01461.x

    Article  CAS  Google Scholar 

  • Kumar SV, Tan SG, Quah SC, Yusoff K (2002a) Isolation of microsatellite markers in mungbean, Vigna radiata. Mol Ecol Notes 2:96–98. doi:10.1046/j.1471-8286.2002.00158.x

    Article  CAS  Google Scholar 

  • Kumar SV, Tan SG, Quah SC, Yusoff K (2002b) Isolation and characterization of seven tetranucleotide microsatellite loci in mungbean, Vigna radiata. Mol Ecol Notes 2:293–295. doi:10.1046/j.1471-8286.2002.00239.x

    Article  CAS  Google Scholar 

  • Liu K, Muse SV (2005) POWERMARKER: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129. doi:10.1093/bioinformatics/bti282

    Article  CAS  PubMed  Google Scholar 

  • Seehalak W, Somta P, Musch W, Srinives P (2009) Microsatellite markers for mungbean developed from sequence database. Mol Ecol Resour (accepted)

  • Somta P, Musch W, Kongsamai B et al (2008) New microsatellite markers isolated from mungbean (Vigna radiata). Mol Ecol Resour 8:1155–1157. doi:10.1111/j.1755-0998.2008.02219.x

    Article  CAS  Google Scholar 

  • Tomooka N, Kaga A, Vaughan DA (2006) The Asian Vigna (Vigna subgenus Ceratotropis) biodiversity and evolution. In: Sharma AK, Sharma S (eds) Plant genome; biodiversity and evolution. Volume 1, part C. Phanerograms (Angiosperms, Dicotyledons). Science Publishers, New Jersey, pp 87–126

    Google Scholar 

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Acknowledgments

P. Somta and W. Seehalak are, respectively, grateful to the Commission on Higher Education, Ministry of Education, Thailand and the National Science and Technology Development Agency (NSTDA), Thailand for providing them with postdoctoral fellowships. We are also acknowledged the financial support from NSTDA to the Project on Biotechnology for Varietal Development of Thai Mungbean. We are also thankful to Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Thailand for providing us with lab facilities.

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Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand

    Prakit Somta, Worapa Seehalak & Peerasak Srinives

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  1. Prakit Somta
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  2. Worapa Seehalak
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  3. Peerasak Srinives
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Correspondence to Peerasak Srinives.

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Prakit Somta and Worapa Seehalak contributed equally to this research.

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Somta, P., Seehalak, W. & Srinives, P. Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers. Conserv Genet 10, 1939–1943 (2009). https://doi.org/10.1007/s10592-009-9860-x

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  • DOI: https://doi.org/10.1007/s10592-009-9860-x

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