Abstract
RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for Jaccard’s similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level. Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package. The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appears to be related to their performance in field conditions for 10 morphological traits that were scored. This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.
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References
Afzal M. A., Haque M. M. and Shanmugasundaram S. 2004 Random amplified polymorphic DNA (RAPD) analysis of selected mungbean (Vigna radiata L. Wilczek) cultivars. Asian J. Pl. Sci. 3, 20–24.
Arunachalam V. 1981 Genetic distance in plant breeding. Indian J. Genet. 14, 226–236.
Bisht I. S., Mahajan R. K. and Kawalkar T. G. 1998 Diversity in mungbean (Vigna radiata L. Wilczek) germplasm collection and its potential use in crop improvement. Ann. Appl. Biol. 132, 301–312.
Chattopadhyay K., Ali M. N., Sarkar H. K., Mandal N. and Bhattacharyya S. 2005 Diversity analysis by RAPD and ISSR markers among the selected mungbean [Vigna radiata (L.) Wilczek] genotypes. Indian J. Genet. Pl. Breed. 65, 173–175.
Lakhanpaul S., Chadha S., Bhat K. V. and Chadha S. 2000 Random amplified polymorphic DNA analysis in Indian mungbean (Vigna radiata L. Wilczek) cultivars. Genetica 109, 227–234.
Manivannan N., Murugan E., Viswanathan P. L. and Dhanakodi C. V. 1998 Genetic divergence in greengram. Legume Res. 21, 131–133.
Mignouna H. D., Ng N. Q., Ikea J. and Thotapilly G. 1998 Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J. Genet. Breed. 53, 151–159.
Newbury H. J. and Ford-Lloyd B. V. 1993 The use of RAPD for assessing variation in plants. Plant Growth Regulation 12, 43–51.
Orozco-Castillo C., Chalmers K. J., Waugh R. and Powell W. 1994 Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87, 934–940.
Page R. D. M. 2001 TreeView (Windows 32) version 1.6.5. Distributed by author. http://taxonomy.zoology.gla.ac.uk/rod/rod.html.
Paredes M., Becerra V., Rojo C., Del Pojo A., Ovalle C. and Aronson J. 2002 Ecotypic differentiation in Medicago polymorpha L. along an environmental gradient in central Chile. RAPDs studies shows little genetic divergence. Euphytica 123, 431–439.
Pavlicek A., Hrda S. and Flegr J. 1999 Free Tree — Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrapping / jackknife analysis of the tree robustness. Application in the RAPD analysis of the genus Frenkelia. Folia Biol. (Praha.) 45, 97–99.
Ramanujam S. 1981 Biometrical basis for yield improvement in mungbean. In: Procedings 1st International Mung bean Symposium, (ed. R. Cowell) pp. 210 AVRDC, Shanhua.
Saini A., Reddy K. S. and Jawali N. 2004 Evaluation of long primers for AP-PCR analysis of mungbean [Vigna radiata (L.) Wilczek]: Genetic relationships and fingerprinting of some genotypes. Indian J. Biotechnol. 3, 511–518.
Santalla M., Power J. B. and Davey M. R. 1998 Genetic diversity in mungbean germplasm revealed by RAPD markers. Plant Breed. 117, 473–478.
Skroch P. W., dos Santos J. B. and Nienhuis J. 1992 Genetic relationships among Phaseolus vulgaris genotypes based on RAPD marker data. Annu. Rep. Bean Improv. Coop. 35, 23–24.
Virk P. S., Ford-Lloyd B. V., Jackson M. T. and Newbury H. J. 1995 Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74, 170–179.
Wachira F. N., Waugh R., Hackett C. A. and Powell W. 1995 Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38, 201–210.
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. and Tingey S. V. 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6231–6235.
Yee E., Kidwell K. K., Sills G. R. and Lumpkin, T. A. 1999 Diversity among selected Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers. Crop Sci. 39, 268–275.
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Lavanya, G.R., Srivastava, J. & Ranade, S.A. Molecular assessment of genetic diversity in mung bean germplasm. J Genet 87, 65–74 (2008). https://doi.org/10.1007/s12041-008-0009-3
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DOI: https://doi.org/10.1007/s12041-008-0009-3