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In vitro rooting of leguminous plants: Difficulties, alternatives, and strategies for improvement

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Abstract

Leguminous plants include many of the most important food crops and are also cultivated for forage, used as a rich fiber source, and are important in the manufacture of pharmaceutical compounds. Their ability to biologically fix atmospheric nitrogen enhances soil fertility. Beyond conventional propagation methods, modern in vitro technology offers new means for propagating these plants. Significant progress has been achieved in somatic embryogenesis and embryo rescue, despite legumes’ notorious recalcitrance to transformation and regeneration of legumes. The establishment of stable and reproducible regeneration protocols has resulted in commercially successful propagation of some legume species. Still, regeneration protocols for many other legumes have not been successful, primarily due to poor in vitro rooting during regeneration. This review addresses the factors constraining rooting, describes alternative methods to promote in vitro rooting, and provides a summary of in vitro rooting studies performed across a large number of leguminous species.

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Authors and Affiliations

  1. Plant Production Department, P.O. Box 2460, College of Food and Agriculture Sciences, King Saud University, Riyadh, 11451, Saudi Arabia

    Yaser Hassan Dewir, Megahed H. Ammar, Salem S. Alghamdi, Nasser A. Al-Suhaibani & Abdullah A. Alsadon

  2. Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt

    Yaser Hassan Dewir

  3. Department of Botany, Karnatak University, Dharwad, 580003, India

    Hosakatte Niranjana Murthy

  4. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 28644, Korea

    Kee Yoeup Paek

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  1. Yaser Hassan Dewir
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  2. Hosakatte Niranjana Murthy
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  3. Megahed H. Ammar
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  4. Salem S. Alghamdi
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  5. Nasser A. Al-Suhaibani
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  6. Abdullah A. Alsadon
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  7. Kee Yoeup Paek
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Correspondence to Yaser Hassan Dewir.

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Dewir, Y.H., Murthy, H.N., Ammar, M.H. et al. In vitro rooting of leguminous plants: Difficulties, alternatives, and strategies for improvement. Hortic. Environ. Biotechnol. 57, 311–322 (2016). https://doi.org/10.1007/s13580-016-0060-6

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  • DOI: https://doi.org/10.1007/s13580-016-0060-6

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