Abstract
A method for in vitro clonal multiplication of Leucaena leucocephala cv K-8 is described. On MS with BAP (3×10−6M), at the optimum temperature of 30°C, shoots from seedling and adult trees multiplied at a rate of 6–7 fold every three weeks. The addition of adenine or glutamine reduced precocious leaf drop. All shoots rooted on MS with IAA (5×10−6M). Micropropagated plants have been successfully transferred to soil.
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References
Arditti J (1977) In: Arditti J (ed) Orchid Biology-Reviews and Perspectives, Cornell Univ. Press, USA, pp 203–293
Bhojwani SS (1980) NZ J Bot 18: 209–214
Bhojwani SS, Mullins K, Cohen D (1985) Acta Hort. (in press)
Bhojwani SS, Razdan MK (1983) Plant Tissue Culture: Theory and practice, Elsevier Science Publishers, Amsterdam
Bray RA (1984) Aust J Exp Animal Husb 24: 379–385
Bristow S (1982) Leucaena Research Reports 3: 28–29
Cohen D, Elliott D (1979) Proc Intl Plant Prop Soc 26: 330–333
Dhawan V, Bhojwani SS (1984) Curr Sci 53: 1159–1161
George EF, Sherrington PD (1984) Plant Propagation by Tissue Culture — Handbook and Directory of Commercial Laboratories, Exegetics Ltd, Hants, England
Glovak L, Greatbatch W (1982) Leucaena Research Reports 3: 81–82
Hu T-W, Liu C-C (1981) Leucaena Research Reports 2: 50
Hughes KW (1981) Env Exp Bot 21: 281–288
Jones RJ (1982) CSIRO Information Service, Sheet No 41-4
Lane WD (1978) Plant Sci Lett 13: 281–285
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Mukhopadhyay A (1984) Ph.D. Thesis, Univ Delhi, Delhi, India
Nagmani R, Venkateswaran S (1983) Leucaena Research Reports 4: 88–89
NRC (1977) Leucaena — Promising Forage and Tree Crop for the Tropics, First ed, National Academy Press, Washington, DC
NRC (1984) Leucaena: Promising Forage and Tree Crop for the Tropics, Second ed, National Academy Press, Washington, DC
Ravishankar GA, Wali A, Grewal S (1983) Leucaena Research Reports 4: 37
Sommer HE, Wetzstein HY (1984) In: Ammirato PV etal. (eds) Handbook of Plant Cell Culture, Vol. 3 Crop species, Macmillan Publ, Co, NY, pp 511–540
Stokes MJ (1980) Proc Intl Plant Prop Soc 30: 255–268
Venkateswaran S, Gandhi V (1979) Proc 32nd Meeting of Tissue Culture Association, Washington DC, pp 118
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Communicated by I. K. Vasil
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Dhawan, V., Bhojwani, S.S. In vitro vegetative propagation of Leucaena leucocephala (Lam.) de Wit. Plant Cell Reports 4, 315–318 (1985). https://doi.org/10.1007/BF00269887
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DOI: https://doi.org/10.1007/BF00269887