Skip to main content
SpringerLink
Log in

Stimulation of in vitro organogenesis from epicotyl explants and successive micropropagation round in Cassia angustifolia Vahl.: an important source of sennosides

  • Published:
Agroforestry Systems Aims and scope Submit manuscript

Abstract

This report describes a protocol for the in vitro shoot induction and plant regeneration from epicotyl explants of Cassia angustifolia on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA), Kinetin and 2-iP (0.5–10.0 μM). MS medium supplemented with BA (5.0 μM) was the most effective in inducing adventitious shoots and growth. The highest rate of shoot multiplication was achieved on MS medium supplemented with BA (5.0 μM) and Indole-3-acetic acid (IAA, 1.0 μM). The nodal segments excised from the shoots regenerated from BA (5.0 μM) and IAA (1.0 μM) were used as explants for next three round of micropropagation. The number of shoots significantly increased at successive round of micropropagation. For rooting, MS medium supplemented with 2.0 μM indole-3-butyric acid proved to be better than that supplemented with IAA or α-naphthalene acetic acid. The in vitro raised plantlets with well developed shoot and roots were successfully established in earthen pots containing garden soil and were grown in greenhouse. About 52 plants (85 %) survived out of 60 plants transferred in garden soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

BA:

6-Benzyladenine

Kin:

Kinetin

2-iP:

2-Isopentenyl adenine

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

PGRs:

Plant growth regulators

References

  • Agrawal V, Sardar PR (2003) In vitro organogenesis and histomorphological investigations in Senna (Cassia angustifolia) a medicinally valuable shrub. Physiol Mol Biol Plants 91:131–140

    Google Scholar 

  • Agrawal V, Sardar PR (2006) In vitro propagation of Cassia angustifolia (Vahl.) through leaflet and cotyledon derived calli. Biol Plant 1:118–122

    Article  Google Scholar 

  • Agrawal V, Sardar PR (2007) In vitro regeneration through somatic embryogenesis and organogenesis using cotyledons of Cassia angustifolia Vahl. In Vitro Cell Dev Biol Plant 43:585–592

    Article  CAS  Google Scholar 

  • Ahmad N, Anis M (2011) An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur J For Res 130:135–144

    Article  CAS  Google Scholar 

  • Ahmad N, Faisal M, Anis M, Aref IM (2010) In vitro callus induction and plant regeneration from leaf explants of Ruta graveolens L. South Afr J Bot 76:597–600

    Article  CAS  Google Scholar 

  • Anonymous (1992) The wealth of India: a dictionary of Indian raw materials and industrial products, vol 3. CSIR, New Delhi, pp 354–363

    Google Scholar 

  • Bohra NK, Sankhla PS (1997) Senna—a cash crop for arid regions. Vaniki Sandesh 21:19–23

    Google Scholar 

  • Chen CU, Hsia CN, Yeh MS, Agrawal DC, Tsay HS (2006) In vitro micropropagation and ex vitro acclimation of Bupleurum kaoi: an endangered medicinal plant native to Taiwan. In Vitro Cell Dev Biol Plant 42:128–133

    Article  CAS  Google Scholar 

  • Dhavala A, Rathore TS (2010) Micropropagation of Embelia ribes Burm f. through proliferation of adult plant axillary shoots. In Vitro Cell Dev Biol Plant 46:180–191

    Article  Google Scholar 

  • Escalettes V, Françoise D (1993) In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Sci 90:201–209

    Article  Google Scholar 

  • Faisal M, Anis M (2003) Rapid mass propagation of Tylophora indica Merill via leaf callus culture. Plant Cell Tissue Organ Cult 75:125–129

    Article  CAS  Google Scholar 

  • Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80:187–190

    Article  CAS  Google Scholar 

  • Franz G (1993) The senna drug and its chemistry. Pharmacology 47:2–6

    Article  PubMed  CAS  Google Scholar 

  • Hung CD, Johnson K, Torpy F (2006) Liquid culture for efficient micropropagation of Wasabia japonica (MIQ.) Matsumura. In Vitro Cell Dev Biol Plant 42:548–552

    Article  CAS  Google Scholar 

  • Jain N, Babbar SB (2000) Recurrent production of plants of black plum, Syzgium cumini (L.) Skeels, a myrtaceous fruit tree from in vitro cultured seedling explants. Plant Cell Rep 19:519–524

    Article  CAS  Google Scholar 

  • Khalafalla MM, Daffalla HM, Abdellatef E, Agabna E, El-Shemy HA (2011) Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir. J Zhejiang Univ Sci B 12:303–312

    Article  PubMed  Google Scholar 

  • Koroch K, Juliani HP, Kapteyn J, Simon JE (2003) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79–83

    Article  Google Scholar 

  • Maharik N, Elgengaini S, Taha H (2009) In vitro mass propagation of the endangered Sinai hawthorn Crataegus sinaica Boiss. Int J Acad Res 1:24–29

    Google Scholar 

  • Mongomake K, Hilaire KT, Daouda K, Michel Z, Justin KY, Ochatt SJ (2009) In vitro plantlets regeneration in Bambara groundnut [Vigna subterranean (L.) Verdc. (Fabaceae)] through direct shoot bud differentiation on hypocotyl and epicotyls cuttings. African J Biotech 8:1466–1473

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Oliveira YD, Pinto F, Lopes da Silva AL, Guedes I, Biasi LA, Quoirin M (2010) An efficient protocol for micropropagation of Melaleuca alternifolia Cheel. In Vitro Cell Dev Biol Plant 46:192–197

    Google Scholar 

  • Pence VC (1999) The application of biotechnology for the conservation of endangered plants. In: Benson EE (ed) Plant conservation biotechnology, vol 15. Taylor and Francis, London, pp 227–241

    Google Scholar 

  • Popelka JC, Gollasch S, Moore A, Molvig L, Higgins TJV (2006) Genetic transformation of cowpea (Vigna unguiculata L.) and stable transmission of the transgenes to progeny. Plant Cell Rep 25:304–312

    Article  PubMed  CAS  Google Scholar 

  • Rout GR (2004) Effect of cytokinins and auxins on micropropagation of Clitorea ternatea L. Biol Lett 41:21–26

    CAS  Google Scholar 

  • Rout GR, Reddy GM, Das P (2001) Studies on in vitro clonal propagation of Paulownia tomentosa Steud, and evaluation of genetic fidelity through RAPD marker. Silvae Genet 50:208–212

    Google Scholar 

  • Shailendra V, Joshi N, Tak K, Purohit SD (2005) In vitro adventitious shoot bud differentiation and plantlet regeneration in Feronia limonia L. (swingle). In Vitro Cell Dev Biol Plant 41:296–302

    Article  Google Scholar 

  • Sharma AK, Goyal RK, Gupta JP (1999) Senna the best choice for sandy wastelands. Indian Farming 6:18–20

    Google Scholar 

  • Siddique I, Anis M (2007a) High frequency multiple shoot regeneration and plantlet formation in Cassia angustifolia (Vahl.) using thidiazuron. Med Arom Plant Sci Biotech 1:282–284

    Google Scholar 

  • Siddique I, Anis M (2007b) In vitro shoot multiplication and plant regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol Plant 29:233–238

    Article  CAS  Google Scholar 

  • Siddique I, Anis M (2008) An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiol Plant 30:493–499

    Article  CAS  Google Scholar 

  • Siddique I, Anis M, Aref IM (2010) In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl. a potential medicinal plant. Appl Biochem Biotechnol 162:2067–2074

    Article  PubMed  CAS  Google Scholar 

  • Somera DA, Samac DA, Olhoft PM (2003) Recent advances in legume transformation. Plant Physiol 131:892–899

    Article  Google Scholar 

  • Tsay HS, Gau TG, Chen CC (1989) Rapid clonal propagation of Pinellia ternata by tissue culture. Plant Cell Rep 8:450–454

    Article  Google Scholar 

Download references

Acknowledgments

This research project was supported by a grant from the research centre of the centre for female scientific and medical colleges in King Saud University.

Author information

Authors and Affiliations

  1. Department of Botany and Microbiology, College of Science, Female Centre for Scientific and Medical Colleges, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia

    Iram Siddique & Muneera R. Al-Othman

  2. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Saad Bin Javed & M. Anis

Authors
  1. Iram Siddique
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saad Bin Javed
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Muneera R. Al-Othman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Anis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Iram Siddique.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siddique, I., Javed, S.B., Al-Othman, M.R. et al. Stimulation of in vitro organogenesis from epicotyl explants and successive micropropagation round in Cassia angustifolia Vahl.: an important source of sennosides. Agroforest Syst 87, 583–590 (2013). https://doi.org/10.1007/s10457-012-9578-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10457-012-9578-5

Keywords

Search

Navigation