Skip to main content
SpringerLink
Log in

Micropropagation of Eucalyptus

  • Chapter
High-Tech and Micropropagation V

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 39))

Abstract

In the past two decades, numerous studies have been conducted on the in vitro propagation of Eucalyptus species (see reviews McComb and Bennett 1986; Le Roux and van Staden 1991; Hartney and Svensson 1992; Curir et al. 1995). Interest in the culture of these species has been for horticultural, ornamental, conservation and especially forestry purposes (Lubrano 1988). Until about 40 years ago, it was often said that it is not possible to propagate Eucalyptus vegetatively (Pryor and Willing 1963). Since then, not only has propagation been possible using traditional methods such as cuttings, air-layering and grafting, but techniques for micropropagation have developed to such an extent that they can now be considered as an essential part of most tree-breeding programs (Hartney and Svensson 1992). The purpose of this chapter is to summarize the recent literature regarding the micropropagation of Eucalyptus in an attempt to determine current trends and to determine the feasibility of using this technique as a tool to supplement the commercial propagation of this species.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Adam S (1987) Obtention de racines transformées chez Eucalyptus gunnii H. par Agrobacterium rhizogenes. Ann Rech Silvicoles, Association Forét-Cellulose 1986, Paris, pp 7–21

    Google Scholar 

  • Ahuja A, Grewal S (1983) Biochemical markers for in vitro initiation of cultures in Eucalyptus macrorhyncha and Eucalyptus youmanii. Natl Acad Sci Lett India 6: 185–187

    CAS  Google Scholar 

  • Aneja S, Atal C (1969) Plantlet formation in tissue cultures from lignotubers of Eucalyptus citriodora Hook. Curr Sci 38: 69–70

    Google Scholar 

  • Arya HC, Shekhawat NS (1986) Clonal multiplication of tree species in the Thar desert through tissue culture. For Ecol Manage 16: 201–208

    Article  CAS  Google Scholar 

  • Bachelard EP, Stowe BB (1963) Growth in vitro of roots of Acer rubrum L. and Eucalyptus camaldulensis Dehnh. Physiol Plant 16: 20–30

    Article  CAS  Google Scholar 

  • Badia NK (1982a) Eucalyptus rudis Endl: techniques de micropropagation par la culture de noeuds in vitro. In: Colloq Int sur la Culture in vitro des Essences Forestieres, IUFRO Nangis, France Association Forét-Cellulose. (AFOCEL), Paris, pp 135–142

    Google Scholar 

  • Badia NK (1982b) Influence de la vitamine E sur la multiplication végétative in vitro de l’ Eucalyptus rudis Endl., de Larix x eurolepis Henry et de Quercus borealis Michx. Bull Rech Agron Gembloux 17: 219–226

    CAS  Google Scholar 

  • Bajaj YPS (ed) (1995) Biotechnology in agriculture and forestry, vol 30. Somatic embryogenesis and synthetic seed I. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Barker PK, de Fossard RA, Bourne RA (1977) Progress towards clonal propagation of Eucalyptus species by tissue culture techniques. Combined Proc Int Plant Propagator’s Soc 27: 546–556

    Google Scholar 

  • Bell GT, van der Moezel PG, Bennett IJ, McComb JA, Wilkins CF, Marshall SCB, Morgan AL (1993) Comparisons of growth of Eucalyptus camaldulensis from seeds and tissue culture: root, shoot and leaf morphology of 9-month-old plants grown in deep sand and sand over clay. For Ecol Manage 57: 125 139

    Google Scholar 

  • Bennett IJ, McComb JA (1982) Propagation of jarrah (Eucalyptus marginata) by organ and tissue culture. Aust For Res 12: 121–127

    Google Scholar 

  • Bennett IJ, Tonkin CM, Wroth MM, Davidson EM, McComb JA (1986) A comparison of growth of seedling and micropropagated Eucalyptus marginata (Jarrah). I. Early growth to 2 years. For Ecol Manage 14: 1–12

    Article  Google Scholar 

  • Bennett II, McComb JA, Tonkin CM, McDavid DAJ (1994) Alternating cylokinins in multiplication media stimulates in vitro shoot growth and rooting of Eucalyptus globulus Labill. Ann Bot 74: 53–58

    PubMed  CAS  Google Scholar 

  • Blakeway FC, Herman B, Watt MP (1993) Establishment of cell suspension cultures of Eucalyptus grandis and E. grandis x camaldulensis. S Afr For J 166: 17–26

    Google Scholar 

  • Blomstedt C, Cameron J, Whiteman P, Chandler SF (1991) Micropropagation of juvenile Eucalyptus regnans ( Mountain Ash ). Aust J Bot 39: 179–186

    Google Scholar 

  • Bonga JM (1982) Vegetative propagation in relation to juvenility, maturity and rejuvenation. In

    Google Scholar 

  • Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Martinus Nijhoff, Dordrecht, pp 387–412

    Google Scholar 

  • Bonner J (1942) Culture of isolated roots of Acacia melanoxvlon. Bull Torrey Bot Club 69: 130–133

    Article  Google Scholar 

  • Boulay M (1983) Micropropagation of frost resistant Eucalyptus. In: Proc Workshop of Eucalyptus. California Sacramento USDA Forest Service General Tech Rep PSW 69, Sacramento,pp 102–107

    Google Scholar 

  • Boulay M (1987) Recherches preliminaires sur l’embryogenèse somatique d’Eucalyptus gunnii. Ann Rech Silvicoles Association Foret-Cellulose (AFOCEL) 1986, Paris, pp 23–37

    Google Scholar 

  • Boxus PH, Terzi JM, Elevens Ch, Pylyser M, Ngaboyamahina P, Duhem K (1991) Improvement and perspectives of micropropagation techniques applied to some hot-climate plants. Acta Hortic 289: 55–59

    Google Scholar 

  • Brackpool AL, Ward MR (1990) Optimisation of adventitious shoot formation for transformation in Eucalyptus. Abstr VII [nt Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No A1–23, p9

    Google Scholar 

  • Burger DW (1987) In vitro micropropagation of Eucalyptus sideroxylon. HortScience 22: 496–497

    CAS  Google Scholar 

  • Chang SH, Donald DGM, Jacobs G (1992) Micropropagation of Eucalyptus radial(’ ssp. radiata using explants from mature and coppice material. S Afr For J 162: 43–47

    Google Scholar 

  • Cheng B, Petersen CM, Mitchell RJ (1992) The role of sucrose, auxin and explant source on in vitro rooting of seedling explants of Eucalyptus sideroxylon. Plant Sci 87: 207–214

    Article  CAS  Google Scholar 

  • Cresswell RJ, de Fossard RA (1974) Organ culture of Eucalyptus grandis. Aust For 37: 55–69

    Google Scholar 

  • Cresswell RJ, Nitsch C (1975) Organ culture of Eucalyptus grandis L. Planta 125: 87 90

    Google Scholar 

  • Curir P, Damiano C, Volpi L, Ruffoni B (1985) Osservazioni sullo sviluppu di nuovi germogli dagemme ascellari nella propagazione in vitro di Eucalyptus gunnii Hook. Ann 1st Sper Floric 16: 59 68

    Google Scholar 

  • Curir P, Damiano C, Esposito P, Ruffoni B (1986) Propagazione in vitro di Eucalyptus gunnii Hook. e di Eucalyptus stuartiana F.V.M. Ann Est Sper Florio 17: 73–84

    Google Scholar 

  • Curir P, Van Sumere CF, Termini A, Barthe P, Marchesini A, Dolci M (1990) Flavonoid accumulation is correlated with adventitious root formation in Eucalyptus gunnii Hook micro-propagated through axillary bud stimulation. Plant Physiol 92: 1148–1153

    Article  PubMed  CAS  Google Scholar 

  • Curir P, Beruto M, Dolci M (1995) Eucalyptus species: In vitro culture and the production of essential oils and other secondary metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 33. Medicinal and aromatic plants VIII. Springer, Berlin Heidelberg New York, pp 194–214

    Google Scholar 

  • Damiano C, Curir P, Esposito P, Ruffoni B (1989) Present micropropagation research programs at I.S.F. in Sanremo. Acta Hortic 251: 129–133

    Google Scholar 

  • Das T, Mitra GC (1990) Micropropagation of Eucalyptus tercticornis Smith. Plant Cell Tissue Organ Cult 22: 95–103

    Article  CAS  Google Scholar 

  • Debergh P, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R, Ziv M (1992) Reconsideration of the term “vitrification” as used in micropropagation. Plant Cell Tissue Organ Cult 30: 135–140

    Article  Google Scholar 

  • De Fossard RA (1974) Tissue culture of Eucalyptus. Aust For 37: 43–54

    Google Scholar 

  • De Fossard RA (1976) Tissue culture for plant propagators. Armidale University of New England, Armidale, NSW, Australia

    Google Scholar 

  • De Fossard RA (1981) Tissue culture propagation of EucalyptusJicifolia F. Muell. Proc Symp Plant Tissue Culture, Peking. Pitman, Boston, pp 425–438

    Google Scholar 

  • De Fossard RA, Bourne RA (1976) Vegetative propagation of Eucalyptus Jiciiblia F. Muell by nodal culture in vitro. Combined Proc lnt Plant Propagator’s Soc 26: 373–378

    Google Scholar 

  • De Fossard RA, Boume RA (1977) Clonal propagation of Eucalyptus by nodal culture. Third World Consultation on Forest Trce Breeding, Canberra, pp 1023–1029

    Google Scholar 

  • De Fossard RA, Myint A, Lee ECM (1974a) A broad spectrum experiment with tobacco (Nicotiana tabacum) pith tissue callus. Physiol Plant 31: 125–130

    Article  Google Scholar 

  • De Fossard RA, Nitsch C, Cresswell RJ, Lee ECM (1974b) Tissue and organ culture of Eucalyptus. N Z J For Sci 4: 267–278

    Google Scholar 

  • De Fossard RA, Barker PK, Bourne RA (1977) The organ culture of nodes of four species of Eucalyptus. Acta Hortic 78: 157–165

    Google Scholar 

  • De Fossard RA, Bennett MT, Gorst JR, Bourne RA (1978) Tissue culture propagation of Eucalyptus ficifolia F. Muell. Combined Proc Int Plant Propagator’s Soc 28: 427–435

    Google Scholar 

  • Denison NP, Kietzka JE (1993) The development and utilisation of vegetative propagation in Mondi for commercial afforestation programmes. S Afr For J 166: 53–60

    Google Scholar 

  • Depommier D (1982) Culture in vitro d’ Eucalyptus résistant au froid; influence de quelques facteurs sur l’allongement et l’enracinement des plantules. Colloq Int sur la Culture in vitro des Essences Forestieres IUFRO. Nangis, Association Forét-Cellulose ( AFOCEL ), Paris, pp 127–132

    Google Scholar 

  • Diallo N, Duhoux E (1984) Organogenése et multiplication in vitro chez 1’ Eucalyptus camaldulensis. J Plant Physiol 115: 177–182

    Article  PubMed  CAS  Google Scholar 

  • Donald DGM, Newton DJ (1991) In vitro propagation of Eucalyptus radiata. S Afr For J 157: 6668

    Google Scholar 

  • Dos Santos AVP, D’Utra Vaz FB, Power JB (1990) Towards somatic hybridization in the genus Eucalyptus. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No A8–17, p 208

    Google Scholar 

  • Durand-Cresswell RJ (1977) The vegetative propagation of Eucalyptus by organ culture. Ph D Thesis Univeristy of New England, Armidale, NSW Australia

    Google Scholar 

  • Durand-Cresswell RJ, Boudet AM (1979) Le bouturage in vitro de I’ Eucalyptus. Etud Rech Association Forét-Cellulose ( AFOCEL ), Paris, 12: 57–66

    Google Scholar 

  • Durand-Cresswell RJ, Nitsch C (1977) Factors influencing the regeneration of Eucalyptus grandis by organ culture. Acta Hortic 78: 149–155

    Google Scholar 

  • Durand-Cresswell RJ, Boulay M, Franclet A (1982) Vegetative propagation of Eucalyptus. In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Nijhoff, The Hague, pp 150–181

    Google Scholar 

  • Fine M (1968) The control of tracheary element formation in Eucalyptus tissue cultures. Ph D Thesis, Yale University, New Haven

    Google Scholar 

  • Franclet A (1956) Premiers travaux d’amélioration génétique des Eucalyptus. Ann Rech For Rabat Maroc 1: 65–89

    Google Scholar 

  • Franclet A (1991) Biotechnology in “rejuvenation”: hope for the micropropagation of difficult woody plants. Acta Hortic 289: 273–282

    Google Scholar 

  • Franclet A, Boulay M (1982) Micropropagation of frost resistant eucalypt clones. Aust For Res 13: 83–89

    Google Scholar 

  • Franclet A, Boulay M (1989) Rejuvenation and clonal silviculture for Eucalyptus and forest species harvested through short rotation. In: Pereira JS, Landsberg JJ (eds) Biomass Production by Fast-Growing Trees. Kluwer, Dordrecht, pp 267–274

    Chapter  Google Scholar 

  • Fromm ME, Taylor LP, Walbot T (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82: 5824–5828

    Article  PubMed  CAS  Google Scholar 

  • Furze MJ, Cresswell CF (1985) Micropropagation of Eucalyptus grandis and E. nitens using tissue culture techniques. S Afr For J 135: 20–23

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Gautheret RJ (1990) Botanique-culture du tissu cambial. In: Dore C (ed) Cinquantenaire de la culture in vitro, vol 51 IN RA, Paris, pp 21–22

    Google Scholar 

  • Goncalves AN (1975) The growth and developmental physiology of Eucalyptus in cell and tissue culture systems. MSc Thesis, Ohio State University, Columbus

    Google Scholar 

  • Goncalves AN (1980) Reversion to juvenility and cloning of Eucalyptus urophylla S.T. Blake in cell and tissue culture systems. IUFRO Symp and Worksh on Genetic improvement and productivity of fast growing tree species. Sao Paulo, Brazil pp 786–787

    Google Scholar 

  • Goncalves AN, Machado MA, Caldas LS, Sharp WR, Mello AH (1979) Tissue culture of Eucalyptus. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture: principles and applications. Ohio State University, Columbus, pp 509–526

    Google Scholar 

  • Grattapaglia D, Caldas LS, Machado MA, Assis TF (1990) Large scale micropropagation of Eucalyptus species and hybrids. Abstr VII Int Congr Plant tissue and cell culture, Amsterdam, Abstr No A3–123, p 113

    Google Scholar 

  • Grewal S, Ahuja A, Atal CK (1980) In vitro proliferation of shoot apices of Eucalyptus citriodora Hook. Indian J Exp Biol 18: 775–777

    Google Scholar 

  • Gupta PK, Mascarenhas AF (1983) Essential oil production in relation to organogenesis in tissue culture of Eucalyptus citriodora Hook. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum, New York, pp 299–308

    Google Scholar 

  • Gupta PK, Mascarenhas AF (1987) Eucalyptus. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 3, Case histories: gymnosperms, angiosperms and palms. Martinus Nijhoff, Dordrecht, pp 385–399

    Google Scholar 

  • Gupta PK, Nadgauda RS, Hendre RR, Mascarenhas AF, Jagannathan V (1978) Multiplication of Eucalyptus citriodora using seedling segments. Proc of the All India Symp and 3rd Conf of Tissue Culture, Baroda, pp 63–64

    Google Scholar 

  • Gupta PK, Mascarenhas AF, Jagannathan V (1981) Tissue culture of forest trees: clonal propagation of mature trees of Eucalyptus citriodora Hook. by tissue culture. Plant Sci Lett 20: 195–201

    Article  Google Scholar 

  • Gupta PK, Mehta UJ, Mascarenhas AF (1983) A tissue culture method for rapid clonal propagation of mature trees of Eucalyptus torelliana and Eucalyptus carnaldulensis. Plant Cell Rep 2: 296–299

    Article  CAS  Google Scholar 

  • Hartney VJ (1980) Vegetative propagation of the eucalypts. Aust For Res 10: 191–211

    Google Scholar 

  • Hartney VJ (1982a) Tissue culture of Eucalyptus Combined Proc Int Plant Propagator’s Soc 32:98–109

    Google Scholar 

  • Hartney VJ (1982b) Vegetative propagation of Eucalyptus in vitro. Colloq Int sur la Culture in vitro des Essences Forestieres IUFRO, Nangis, Association Foret-Cellulose, ( AFOCEL ), Paris, pp 175–179

    Google Scholar 

  • Hartney VJ, Barker PK (1980) Vegetative propagation of Eucalyptus by tissue culture. IUFRO Symp and Workshop on Genetic Improvement and Production of Fast Growing Tree Species, Sao Paulo, pp 791–793

    Google Scholar 

  • Hartney VJ, Svensson JGP (1992) The role of micropropagation for Australian tree species. In: Baker FNG (ed) Rapid propagation of fast-growing woody species. Proc Symp held in 1989. CASAFA Rep Ser No 3 Nallingford, pp 7–28

    Google Scholar 

  • Heller R (1953) Recherches sur la nutrition minerale des tissus végétaux cultivés in vitro. Ann Sci Nat Biol Veg 14: 1–223

    Google Scholar 

  • Holden PG, Paton DM (1981) Sterilization of field-grown Eucalyptus for organ culture. J Aust Inst Hortic 3: 5–7

    Google Scholar 

  • Ihsan I, Shabana J (1987) Mass propagation of Eucalyptus tereticornis Smith. Pak J Bot 19: 67–74

    Google Scholar 

  • Ikemori YK (1987) Epicormic shoots from the branches of Eucalyptus grandis as an explant source for in vitro culture. Comm For Rev 66: 351–355

    Google Scholar 

  • Ishii K (1982) Isoenzyme polymorphism of peroxidasc of Eucalyptus callus in relation to plant hormone contents. In: Fujiwara A (ed) Proc 5th Int Congr Plant tissue and cell culture, Maruzen, Tokyo, pp 215–216

    Google Scholar 

  • Ito K, Doi K, Tatemichi Y, Shibata M (1990) Plant regeneration from protoplasts of Eucalyptus. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No AI-65 p 19

    Google Scholar 

  • Jacquiot C (1964a) Application de la technique de culture des tissues végétaux à l’étude dc quelques problémes de la physiologie de l’abre. Ann Sci For 21: 310–473

    Article  Google Scholar 

  • Jacquiot C (1964b) Structure of excised roots or of organs formed de novo from cambial tissue of trees grown in culture. Rev Cytol Biol Veg 27: 319–322

    Google Scholar 

  • Janick J (1986) Horticultural science, 4th edn. WH Freeman, New York

    Book  Google Scholar 

  • Jones NB, van Staden J (1994) Micropropagation and establishment of Eucalvptusgrandis hybrids. S Afr J Bot 60: 122–126

    Google Scholar 

  • Jones NB, Drennan PM, van Staden J (1993) Leaf anatomy, chloroplast organization and pho- tosynthetic rate of hyperhydric Eucalyptus saligna sm. material. S Afr J Bot 59: 551–555

    Google Scholar 

  • Kawazu T, Doi K, Ohta T, Shinohara Y, Ito K, Shibata M (1990) Transformation of Eucalyptus (Eucalyptus saligna) using electroporation. Abstr VII Int Congr Plant tissue and cell culture, Amsterdam, Abstr No A2–80, p 64

    Google Scholar 

  • Kevers C, Prat R, Gaspar Th (1987) Vitrification of carnation in vitro: changes in cell wall mechanical properties, cellulose and lignin content. Plant Growth Regul 5: 59–66

    Article  CAS  Google Scholar 

  • Kevers C, Goldberg R, Chu-Ba J, Gaspar Th (1988) Composition of the walls of stems and leaves of vitrifying carnation. Biol Plant 30: 219–223

    Article  CAS  Google Scholar 

  • Khanna P, Staba EJ (1968) Antimicrobials from plant tissue cultures. Lloydia 31: 180–189

    Google Scholar 

  • Kitahara EH, Caldas LS (1975) Shoot and root formation in hypocotyl callus cultures of Eucalyptus. For Sci 21: 242–243

    CAS  Google Scholar 

  • Knop W (1865) Quantitative Untersuchungen fiber die Ernährungsprozesse der Pflanzen. Land-wirtsch Vers Stn 7: 93–107

    Google Scholar 

  • Kumar RR, Raman K (1989) Callus induction and recovery of plantlets from seedling explants of Eucalyptus camaldulensis Dehn. J Plant Crops 16: 21–26

    Google Scholar 

  • Lakshmi Sita G (1979) Morphogenesis and plant regeneration from cotyledonary cultures of Eucalyptus. Plant Sei Lett 14: 63–68

    Article  Google Scholar 

  • Lakshmi Sita G, Rani BS (1985) In vitro propagation of Eucalyptus grandis. L. by tissue culture. Plant Cell Rep 4: 63–65

    Google Scholar 

  • Lakshmi Sita G, Vaidyanathan CS (1979) Rapid multiplication of Eucalyptus by multiple shoot production. Curr Sci 48: 350–352

    Google Scholar 

  • Lakshmi Sita G, Rani S, Rao KS (1986) Propagation of Eucalyptus grandis by tissue culture. In: Sharma JK, Nair CTS, Kedharnath S, Kondas S (eds) Eucalyptus in India. past, present and future. Proc Natl Semi Kerala Forest Resh Inst. Peechi Kerala, pp 318–321

    Google Scholar 

  • Lee ECM, de Fossard RA (1974) The effects of various auxins and cytokinins on the in vitro culture of stem and lignotuber tissue of Eucalyptus hancroftii Maiden. New Phytol 73: 707–717

    Article  CAS  Google Scholar 

  • Le Roux JJ, van Staden J (1991) Micropropagation and tissue culture of Eucalyptus a review. Tree Physiol 9: 435–477

    Article  PubMed  Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127

    Article  CAS  Google Scholar 

  • Lubrano L (1988) Micropropagation of Eucalyptus x trabutti, E. viminalis and E. gunnü. Acta Hortic 227: 428–429

    Google Scholar 

  • Lubrano L (1992) Micropropagation of Eucalyptus. Cellul Carta 43: 27–33

    Google Scholar 

  • Macrae S, van Staden J (1993) Agrobacterium rhizogenes-mediated transformation to improve rooting ability of Eucalyptus Tree Physiol 12:411–418

    Article  PubMed  Google Scholar 

  • Maguchi T, Fukuzumi T (1982) Volatile compounds from callus cells of woody plants. In: Fujiwara A (ed) Plant Tissue Culture 1982. Maruzen, Tokyo, pp 287–288

    Google Scholar 

  • Marcavillaca MC, Montaldi ER (1964) Culture in vitro de tejidos de eucalipto. IDIA 12: 62–64

    Google Scholar 

  • Mascarenhas AF, Hazara S, Potdar U, Kulkarni DK, Gupta PK (1982) Rapid clonal multiplication of mature forest trees through tissue culture. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 719–720

    Google Scholar 

  • McComb JA, Bennett IJ (1982) Vegetative propagation of eucalypts: using tissue culture and its application to forest improvement in Western Australia. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 721–722

    Google Scholar 

  • McComb JA, Bennett IJ (1986) Eucalypts (Eucalyptus spp). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. Trees I. Springer, Berlin Heidelberg New York, pp 340–362

    Google Scholar 

  • McComb JA, Wroth M (1986) Vegetative propagation of Eucalyptus resinifcra and E. maculate using coppice cuttings and micropropagation. Aust For Res 16: 231–242

    Google Scholar 

  • McComb JA, Bennett IJ, van der Moezel PG, Bell DT (1989) Biotechnology enhances utilization of Australian woody species for pulp, fuel, and land rehabiliation. Aust J Biotech 3: 297–301

    Google Scholar 

  • Mehra-Palta A (1982) Clonal propagation of Eucalyptus by tissue culture. Plant Sci Lett 26: 1–11

    Article  CAS  Google Scholar 

  • Mehra-Palta A, Smeltzer RH, Mott RL (1978) Hormonal control of induced organogenesis: experiments with excised plant parts of loblolly pine. TAPPI 61: 37–40

    CAS  Google Scholar 

  • Mohammed GH, Gnbbes SL, Vidaver WE (1992) Ex vitro photosynthetic activity in plantlets of tissue cultured Douglas fir. Tree Physiol 10: 403–410

    Article  PubMed  Google Scholar 

  • Muralidharan EM, Mascarenhas AF (1987) In vitro plantlet formation by organogenesis in Eucalyptus camaldulensis and by somatic embryogenesis in Eucalyptus citriodora. Plant Cell Rep 6: 256 259

    Google Scholar 

  • Muralidharan EM, Gupta PK, Mascarenhas AF (1989) Plantlet production through high frequency somatic embryogenesis in long-term cultures of Eucalyptus citriodora. Plant Cell Rep 8: 41–43

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nash D (1968) A preliminary study of the metabolism of Paul’s Scarlet rose cells in suspension culture. PhD Thesis, University of Birmingham, Birmingham

    Google Scholar 

  • Nash DT, Davis ME (1972) Some aspects of growth and metabolism of Paul’s Scarlet rose cell suspensions. J Exp Bot 23: 75–91

    Article  CAS  Google Scholar 

  • Nitsch C, Nitsch JP (1967) The induction of flowering in vitro in stem segments of Plurnhago indica L. 11. The production of reproductive buds. Planta 72: 371–384

    Google Scholar 

  • Nitsch JP (1951) Growth and development in vitro of’ excised ovaries. Am J Bot 38:566 577 Norstog K, Rhamstine E (1967) Isolation and culture of haploid and diploid cycad tissue. Phytomorphology 17: 374–381

    Google Scholar 

  • Oka S, Yeung EC, Thorpe TA (1982) Shoot formation in Eucalyptus glohulus hypocotyl explants. N Z J For Sci 12: 501–509

    Google Scholar 

  • Oliphant J (1990) The use of paclobutrazol in the rooting medium of micropropagated plants. Int Plant Propagators’ Soc 40: 358–360

    Google Scholar 

  • Orihara Y, Miyatake H, Furuya T (1991) Triglucosylation on the biotransformation of (+) menthol by cultured cells of Eucalyptus perriniana. Phytochemistry 30: 1843–1845

    Article  PubMed  CAS  Google Scholar 

  • Ouyang Q, Li QQ, Peng HZ (1980) Preliminary report on the development of embryoids from Eucalyptus. Acta Phytophysiol Sin 6: 429–432

    Google Scholar 

  • Ouyang Q, Peng HS, Li QQ (1981) Studies on the development of embryoids from Eucalyptus callus. Sci Silvae Sin 17: 1–7

    Google Scholar 

  • Penchel RM, Kirby EG (1990a) Establishment of cell culture systems for selected superior hybrids of Eucalyptus grandis. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No Al-113, p31

    Google Scholar 

  • Penchel RM, Kirby EG (1990b) Isolation and culture of protoplasts from juvenile and adult Eucalyptus grandis hybrids. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No AI-114, p 31

    Google Scholar 

  • Piton F (1969) Quelques observation cytologiques sur des tissues d’ Eucalyptus catnaldulensis cultivés in vitro. Rev Gen Bot 76: 287–307

    Google Scholar 

  • Potts BM, Potts WC (1986) Eucalypt breeding in France. Aust For 49: 210–218

    Google Scholar 

  • Pryor LD, Willing RR (1963) The vegetative propagation of Eucalyptus an account of progress. Aust For 27: 52–62

    Google Scholar 

  • Qin Chang-Le, Kirby EG (1990) Induction of shoots and embryo-like structures in cultures derived from juvenile and adult explants of Eucalyptus spp. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No AI-74, p 21

    Google Scholar 

  • Quoirin M, Lepoivre P (1977) Improved media for in vitro culture of Prunus sp. Acta Hortic 78: 437–442

    Google Scholar 

  • Raghavan P (1986) Plantlet regeneration from callus cultures of Eucalyptus grandis Hill ex Maiden. Curr Sei 55: 741–743

    Google Scholar 

  • Ram H, Rao CH, Mathur JMS (1971) Anthocyanin development in vitro in tender internode explants of Eucalyptus. Curri Sei 18: 499–500

    Google Scholar 

  • Rao KS (1988) In vitro meristem cloning of Eucalyptus tereticornis Sm. Plant Cell Rep 7: 546549

    Google Scholar 

  • Rao KS, Venkateswara R (1985) Tissue culture of forest trees: clonal multiplication of Eucalyptus’ grandis L. Plant Sci 40: 51–55

    Article  Google Scholar 

  • Salinero MC (1983) Advance in micropropagation of Eucalyptus glohulus by in vitro nodal culture. Colloq Int sur les Eucalyptus Résistants au Froid, Nangis, Association Forét-Cellulose ( AFOCEL ), Paris, pp 609–618

    Google Scholar 

  • Samejima M, Yamaguchi T, Fukuzumi T, Yoshimoto T (1982) Effects of phytohormones on accumulation of flavanols in callus cells of woody plants. In: Fujiwara A (ed) Plant Tissue Culture 1982. Maruzen, Tokyo, pp 353–354

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction of growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204

    Article  CAS  Google Scholar 

  • Strobel GA, Nachmias A (1985) Agrobacterium rhizogenes promotes the initial growth of bare roots stocks in almonds. J Gen Microbiol 131: 1245–1249

    Google Scholar 

  • Subbaiah MM, Minocha SC (1990) Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis. Plant Cell Rep 9: 370–373

    Article  CAS  Google Scholar 

  • Sussex IM (1965) The origin and morphogenesis of Eucalyptus cell populations. In: White PR

    Google Scholar 

  • Grove AR (eds) Proc Int Confer Plant Tissue Culture. McCutchan, Berkeley, pp 383–391

    Google Scholar 

  • Teixeira SL, Da Silva LL (1990) In vitro propagation of adult Eucalyptus grandis Hill. ex Maiden, from epicormic shoots. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No A3–218, 137 pp

    Google Scholar 

  • Teulieres C, Boudet AM (1991) Isolation of protoplasts from different Eucalyptus species and preliminary studies on regeneration. Plant Cell Tissue Organ Cult 25: 133–140

    Google Scholar 

  • Teulieres C, Ferrand D, Boudet AM (1989) Obtention de suspensions cellularies d’ Eucalyptus gunnii: survie des cellules et de leurs protoplastes à basse temperature. Ann Rech Association Foret-Cellulose ( AFOCEL ), Paris, 1988, pp 117–131

    Google Scholar 

  • Teulieres C, Teissie J, Boudet AM (1990) Transient gene expression in protoplasts and intact cells of Eucalyptus gunnii after electrical or chemical treatment. Abstr VII Int Congr Plant tissue and cell culture, Amsterdam, Abstr No A2–136, p 78

    Google Scholar 

  • Texier F, Faucher M (1986) Culture in vitro d’apex d’Eucalyptus age. In: Annales de Retherchers Sylvicoles. Association Foret-Cellulose ( AFOCEL ), Paris, pp 7–23

    Google Scholar 

  • Trindade H, Ferreira J, Pais MS (1990a) In vitro rooting of Eucalyptus glohulus Labill. Abstr VII Int Congr Plant Tissue and Cell Cultue, Amsterdam, Abstr No A3–224, pp 138

    Google Scholar 

  • Trindade H, Ferreira JG, Pais M, Aloni R (1990b) The role of cytokinin and auxin in rapid multiplication of shoots of Eucalyptus glohulus grown in vitro. Aust For 53: 221–223

    Google Scholar 

  • Tripathi BK, Boulay M, Ben Badis A (1983) On the isolated root culture and root bud formation in Eucalyptus viminalis x dalrympeana. Colloq Int sur les Eucalyptus Résistants. Nangis, Association Foret-Cellulose ( AFOCEL ), Paris, 1983, pp 602–608

    Google Scholar 

  • Vieitez AM, Ballester A, San-José MC, Vieitez E (1985) Anatomical and chemical studies of vitrified shoots of chestnut regenerated in vitro. Physiol Plant 65: 177–184

    Article  CAS  Google Scholar 

  • Von Arnold S, Eriksson T (1977) A revised medium for growth of pea mesophyll protoplasts. Physiol Plant 39: 257–260

    Article  Google Scholar 

  • Warrag EL, Lesney MS, Rockwood DJ (1989a) Comparative greenhouse study of Eucalyptus grandis in vitro plantlets and half-sib seedlings. I. Net Photosynthesis. Plant Cell Rep 8: 497 499

    Google Scholar 

  • Warrag EL, Lesney MS, Rockwood DJ (1989b) Comparative greenhouse study of Eucalyptus grandis in vitro plantlets and half-sib seedlings. II. Dry matter accumulation and relative distribution. Plant Cell Rep 8: 500–503

    Google Scholar 

  • Warrag EL, Lesney MS, Rockwood DJ (1990) Micropropagation of field tested superior Eucalyptus grandis hybrids. New For 4: 67–79

    Article  Google Scholar 

  • Warrag EL, Lesney MS, Rockwood DJ (1991) Nodule structure and regeneration of Eucalyptus grandis hybrids. Plant Cell Rep 9: 586–589

    Article  Google Scholar 

  • Watt MP, Blakeway F, Cresswell CF, Herman B (1991) Somatic embryogenesis in Eucalyptus grandis. S Afr For J 157: 59–65

    Google Scholar 

  • Werker E, Leshem B (1987) Structural changes during vitrification of carnation plantlets. Ann Bot 59: 377–385

    Google Scholar 

  • White PR (1943) A handbook of plant tissue culture. Jacques Cattell Press, Lancaster, 277 pp

    Book  Google Scholar 

  • White PR (1954) The cultivation of animal and plant cells, 1st edn. Ronald Press, New York

    Google Scholar 

  • White PR (1963) The cultivation of animal and plant cells, 2nd edn. Ronald Press, New York

    Google Scholar 

  • Winton LL (1974) Second addendum to the bibliography of tree callus cultures. Genetics and Physiology Notes No 19. The Institute of Paper Chemistry, Appleton

    Google Scholar 

  • Young R, Chandler SF (1990) An adventitious shoot regeneration system in Eucalyptus glohulus (Tasmanian Blue Gum): Effects of antibiotics and results of Agrobacterium co-cultivation. Abstr VII Int Congr Plant Tissue and Cell Culture, Amsterdam, Abstr No Al-27, 10 pp

    Google Scholar 

  • Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer, Dordrecht, pp 45–69

    Google Scholar 

  • Ziv M, Meir G, Halevy AH (1983) The development of glaucous hardened carnation plants in vitro. Plant Cell Tissue Organ Cult 2: 55–65

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Unit for Plant Growth and Development, Department of Botany, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, Republic of South Africa

    N. B. Jones & J. Van Staden

Authors
  1. N. B. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Van Staden
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. New Friends Colony, A-137, 110065, New Delhi, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Jones, N.B., Staden, J.V. (1997). Micropropagation of Eucalyptus . In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation V. Biotechnology in Agriculture and Forestry, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07774-0_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-07774-0_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08269-6

  • Online ISBN: 978-3-662-07774-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation