Abstract
In planta differentiation of somatic embryos was induced in seedlings of peanut (Arachis hypogaea L.) obtained from mature seeds germinated on a medium supplemented with thidiazuron (TDZ: N-phenyl-N1- (1,2,3 thiadiazol-yl)urea). At optimum levels of TDZ (10 μM), all germinating seeds produced embryogenic seedlings, and somatic embryos developed in the apical region and on the surface of cotyledons and hypocotyls. These somatic embryos matured, germinated, and formed shoots which eventually developed into whole plants. Thidiazuron-induced direct embryogenesis from morphologically intact seedlings may provide an excellent experimental system for investigating somatic embryogenesis and the morphoregulatory role of TDZ.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog (1962) medium
- TDZ:
-
thidiazuron (N-phenyl-N1(1,2,3 thiadiazol-yl)urea)
References
Capelle, S.C., Mok, D.W.S., Kirchner, S.C., Mok, M.C. (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(2-isopentenyl)[8-14C] adenosine in callus tissue of Phaseolus lunatus L. Plant Physiol. 73, 796–802
Carman, J.G. (1990) Embryogenic cells in plant tissue cultures: Occurrence and behaviour. In Vitro Cell. Dev. Biol. 26, 746–753
Fiola, J.A., Hassan, M.A., Swartz, H.J., Bors, R.H., McNicols, R. (1990) Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant. Cell Tiss. Org. Cult. 20, 223–228
Fujimura T., Komamine, A. (1980) Mode of action of 2,4-D and zeatin on somatic embryogenesis in a carrot cell suspension culture. Z Pflanzenphysiol. 99, 1–8
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. J. Exp. Res. 50, 151–158
Hazra, S., Sathaye, S.S., Mascarenhas, A.F. (1989) Direct somatic embryogenesis in peanut (Arachis hypogea). BioTechnology 7, 949–951
Kato, H. (1968) The serial observations of the adventive embryogenesis in the microculture of carrot tissue. Scient. Pap. Coll. Gen. Educ. Univ. Tokyo 18, 191–197
Malik K.A., Saxena, P.K. (1991) Regeneration in Phaseolus vulgaris L. Promotive role of N6-benzylaminopurine in cultures from juvenile leaves. Planta 184, 148–150
Malik, K.A., Saxena, P.K. (1992a) Regeneration in Phaseolus vulgaris L. High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186, 384–389
Malik, K.A. and Saxena, P.K. (1992b) In vitro regeneration of plants: A novel approach. Naturwissenschaften (in press)
McKently, A.H., Moore, G.A., Gardner, F.P. (1991) Regeneration of peanut and perennial peanut from cultured leaft tissue. Crop Sci. 31, 833–837
Mok, M.C., Mok, D.W.S., Armstrong, D.J., Shudo, K., Isogai, Y., Okanoto, T. (1982) Cytokinin activity of N-phenyl-N′-1,2,3- thidiazol-5-yl-urea (thidiazuron). Phytochemistry 21, 1509–1511
Morrish F., Vasil, V., Vasil, I.K. (1987) Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the Gramineae. Adv. Genet. 24, 431–499
Murashige, T., Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497
Ozias-Akins, P. (1989) Plant regeneration from immature embryos of peanut. Plant Cell Rep. 8, 217–218
Reinert, J. (1959) Uber die Kontrolle der Morphogenese und die Induktion von Adventivembryonen an Gewebekulturen aus Karotten. Planta 53, 318–333
Steward, F.C., Mapes, M.O., Mears, K. (1958) Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Am. J. Bot. 45, 705–708
Steward, F.C., Mapes, M.O., Kent, A.E., Holsten, R.D. (1964) Growth and development of cultured plant cells. Biochemical and morphogenetic studies with cells yield new evidence on their metabolism and totipotency. Science 143, 20–27
Street, H.E. (1979) Embryogenesis and chemically induced organogenesis. In: Plant cell and tissue culture, pp. 123–153, Sharp, W.R., Larsen, P.O., Paddock, E.F., Raghavan, V., eds. Ohio State University Press, Columbus, USA
Thomas, J.C., Katterman, F.R. (1986) Cytokinin activity induced by thidiazuron. Plant Physiol. 81, 681–683
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This research was supported by an operating grant from the Natural and Engineering Research Council of Canada to P.K.S. We thank Drs. J.A. Qureshi and Judith Strommer for helpful discussions, and Sangeeta Saxena for technical assistance. A gift of technical-grade thidiazuron from Nor-Am Chemical Co., Wilmington, Del., USA is gratefully acknowledged.
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Saxena, P.K., Malik, K.A. & Gill, R. Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut. Planta 187, 421–424 (1992). https://doi.org/10.1007/BF00195667
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DOI: https://doi.org/10.1007/BF00195667