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Shoot multiplication and plant regeneration in Caragana fruticosa (Pall.) Besser

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Abstract

Different nutrient media can affect in vitro culturing protocols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first in vitro propagation studies for the endangered species Caragana fruticosa (Fabaceae). We evaluated various nutrient media for their impact on shoot elongation and axillary bud proliferation using different concentrations of 6-benzylaminopurine (BA) and α-naphthaleneacetic acid (NAA). Shoot elongation was evaluated based on adventitious shoot primary culture and subculture regeneration from Caragana seedlings. Our goal was to improve both micropropagation and regeneration in C. fruticosa. MS nutrient media was superior to 1/2MS macronutrients, DKW, QL, and WPM for shoot elongation and axillary shoot proliferation. Shoots grown on 1/2MS and WPM exhibited some chlorosis, and shoots on QL produced larger leavers than plants growing on normal medium. The shoot proliferation coefficient on MS media supplemented with 2.22 μM BA and 0.44 μM BA + 2.69 μM NAA was significantly higher than that with other treatments in the primary culture. Shoots on 2.22 μM BA showed a higher proliferation coefficient (3.17) than others in the subculture. Shoots were rooted on 1/2MS medium with the addition of different concentrations of NAA. The optimal concentration for rooting was 0.27 μM NAA (74%). Roots exhibited many stout and long root hairs. Survivl of established plantlets was 82% at 30 days after transfer to soil. Plants established in the green house showed normal growth and displayed no apparent morphological differences compared to stock plants.

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Abbreviations

1/2MS:

Half strength of MS macronutrients

BA:

6-Benzylaminopurine

DKW:

Driver-Kuniyuki walnut medium

MS:

Murashige and Skoog medium

NAA:

α-Naphthaleneacetic acid

PGRs:

Plant growth regulators

QL:

Quoirin and Lepoivr’s medium

WPM:

Woody Plant Medium

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Authors and Affiliations

  1. School of Forestry, State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China

    Xiao-jie Zhai, Ling Yang & Hai-long Shen

Authors
  1. Xiao-jie Zhai
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  2. Ling Yang
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  3. Hai-long Shen
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Correspondence to Ling Yang.

Additional information

Foundation project: This research was supported by the Key Technologies R&D Program of China during 2006–2010 (2006BAD03A04) and the Fundamental Research Funds for the Central Universities (DL10BA04).

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Zhai, Xj., Yang, L. & Shen, Hl. Shoot multiplication and plant regeneration in Caragana fruticosa (Pall.) Besser. Journal of Forestry Research 22, 561–567 (2011). https://doi.org/10.1007/s11676-011-0199-z

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  • DOI: https://doi.org/10.1007/s11676-011-0199-z

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