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In Vitro Plantlet Regeneration from Alysicarpus monilifer, a Source of Hepatoprotective Drug

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A plantlet regeneration protocol has been developed for Alysicarpus monilifer, a medicinal plant that is a source of hepato-protective drugs. Callus was induced from mature cotyledonary leaves from 4 to 5 days old seedling on Murashige and Skoog (MS) medium supplemented with 1.0 mg l−1 2,4-dichlorophenoxyacetic acid (2, 4-D). Proliferation of cultures occurred on MS medium with 1.0 mg l−1 each of 2,4-D, 6-benzylaminopurine (BA) and kinetin (Kin). Shoot regeneration from proliferated callus was influenced by a number of factors namely plant growth regulators (PGRs), gelling agents, culture vessels and carbohydrate source. The highest (85.6 %) shoot regeneration was recorded in 250 ml culture flasks on agar gelled MS medium + 0.1 mg l−1 α-naphthalene acetic acid (NAA) + 1.0 mg l−1 each of BA, Kin and 2-isopentenyladenine (2iP) + 1 % glucose and 2 % maltose in addition to the usual 3 % sucrose. The shoots differentiated on PGRs, free MS medium, were stronger and longer than the shoots developed on MS medium containing PGRs (0.1 mg l−1 NAA + 1.0 mg l−1 each of BA, Kin and 2iP) with different leaf morphology and were easy to root. Rooting of the regenerated shoots was achieved both in vitro and ex vitro. About 80.4 % of the shoots rooted in vitro on half strength MS medium containing 1.0 mg l−1 indole-3-butyric acid (IBA), while 84.9 % of the shoots rooted under the ex vitro condition when treated with 250 mg l−1 IBA for 5 min. The plants were hardened in the green house and showed 85 % survival rate.

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Acknowledgments

We are thankful to University Grants Commission (UGC), New Delhi and Council of Scientific and Industrial Research (CSIR), New Delhi for financial assistance to authors Nisha Rathore and Nitika Singh Rathore respectively. We also thank Prof. Kamini Dinesh, Retired Professor, Department of English, J.N.V. University, Jodhpur (India), for language improvement of the revised manuscript.

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Authors and Affiliations

  1. Biotechnology Unit, Department of Botany, Jai Narain Vyas University, Jodhpur, 342001, Rajasthan, India

    Nisha Rathore, Nitika Singh Rathore & N. S. Shekhawat

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  1. Nisha Rathore
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  2. Nitika Singh Rathore
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  3. N. S. Shekhawat
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Correspondence to N. S. Shekhawat.

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Rathore, N., Rathore, N.S. & Shekhawat, N.S. In Vitro Plantlet Regeneration from Alysicarpus monilifer, a Source of Hepatoprotective Drug. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 815–822 (2014). https://doi.org/10.1007/s40011-013-0278-3

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  • DOI: https://doi.org/10.1007/s40011-013-0278-3

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