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High-resolution mapping of a gene conferring strong antibiosis to brown planthopper and developing resistant near-isogenic lines in 9311 background

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Abstract

The brown planthopper (BPH), Nilaparvata lugens Stål, is one of the most destructive pests to the rice production in the world. Thus, there is an urgency to identify new resistant genes for breeding. AC-1613 is an indica variety that has been reported to confer broad-spectrum resistance to BPH. In the present study, we found that AC-1613 exhibited strong antibiosis towards BPH insects. The body weight was significantly decreased when the insects fed on AC-1613 plants. By using BPH weight gain as an index of phenotyping, a novel dominant locus for resistance to BPH, designed as Bph30, was identified and its near-isogenic line (NIL) in 9311 background was developed. The F2 population derived from a cross between AC-1613 and 9311 was used for mapping the gene. Through QTL scan, we located the gene on the short arm of chromosome 4 between RM16278 and RM16425, which explained 42.7% of the phenotypic variance (PEV) of BPH resistance in the F2 population. The gene was finally located in a region flanking by simple sequence repeat (SSR) markers SSR-28 and SSR-69 through high-resolution mapping, the distance between the two markers in Nipponbare genome is 37.5 kb. In addition, SSR markers RM16294 and RM16299 tightly linked to Bph30 were applied effectively in introgressing Bph30 into elite rice cultivars. The developed NILs showed a strong antibiosis and high resistance to BPH.

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Acknowledgements

This study was supported by grants from the National Program on Research & Development of Transgenic Plants (2016ZX08009-003-001), the National Natural Science Foundation of China (31630063), and the National Key Research and Development Program (2016YFD0100600).

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  1. Huiying Wang and Shaojie Shi contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Huiying Wang, Shaojie Shi, Qin Guo, Lingyun Nie, Bo Du, Rongzhi Chen, Lili Zhu & Guangcun He

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  1. Huiying Wang
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  2. Shaojie Shi
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Corresponding author

Correspondence to Guangcun He.

Electronic supplementary material

Fig. S1

The pedigree for developing mapping populations and NILs (PNG 70.1 kb)

High-resolution image (TIF 1482 kb)

Fig. S2

Overview of Parafilm sachet test of the BPH. a The distant view and b the close shot of Parafilm sachet test of the BPH. The labels with ‘L86’ and ‘L102’ denote recombinant individuals from mapping population (PNG 2483 kb)

High-resolution image (TIF 8403 kb)

Fig. S3

High-resolution mapping of Bph30 gene of part recombinants in BC2F2 populations. The upper local linkage map was constructed by seven SSR markers, scale bar = 20 kb. The below graphical presentation indicates the genotypes and phenotypes of the recombinants. The white and gray bars indicate the marker genotypes of 9311 homozygotes and heterozygotes, respectively. Weight gains indicate mean ± SD, n ≥ 3 repeats (PNG 123 kb)

High-resolution image (TIF 1945 kb)

Fig. S4

Genetic background assay of BC6F1 using the RICE6K array. The red lines indicate the SNP loci with homozygous AC-1613 genotypes and the blue lines indicate the SNP loci with heterozygous genotypes. The asterisk (*) denotes the position of Bph30 locus. (PNG 34.8 kb)

High-resolution image (TIF 6264 kb)

Table S1

Populations designated for identification and mapping of Bph30 (DOCX 18.9 kb)

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Wang, H., Shi, S., Guo, Q. et al. High-resolution mapping of a gene conferring strong antibiosis to brown planthopper and developing resistant near-isogenic lines in 9311 background. Mol Breeding 38, 107 (2018). https://doi.org/10.1007/s11032-018-0859-1

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