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High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds

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Abstract

Brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive insect pests of rice. Exploring resistance genes from diverse germplasms and incorporating them into cultivated varieties are critical for controlling this insect. The rice variety Swarnalata was reported to carry a resistance gene (designated Bph6), which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown. In this study, we identified and mapped this gene using the F2 and backcrossing populations and characterized its resistance in indica 9311 and japonica Nipponbare using near isogenic lines (NILs). In analysis of 9311/Swarnalata F2 population, the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742. The gene was further mapped precisely to a 25-kb region delimited between the STS markers Y19 and Y9; and the distance between these markers is 25-kb in Nipponbare genome. The Bph6 explained 77.5% of the phenotypic variance of BPH resistance in F2 population and 84.9% in BC2F2 population. Allele from Swarnalata significantly increased resistance to the BPH, resulted in a reduced damage score. In characterization of Bph6-mediated resistance, the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release. BPH growth and development were inhibited, and the insect’s survival rates were lower on Bph6-NIL plants, compared with the parents 9311 and Nipponbare. The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants, and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant no. 30671287), the National High Technology Research and Development Program of China (grant no. 2006AA10Z144) and the Special Fund for Public Industry from the Ministry of Agriculture of China (grant no. 200803003) and the National Major Project of Breeding for New Transgenic Organisms (grant no. 2009ZX08009-047B).

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  1. Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Yongfu Qiu, Jianping Guo, Shengli Jing, Lili Zhu & Guangcun He

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  1. Yongfu Qiu
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Correspondence to Guangcun He.

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Communicated by Y. Xu.

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Supplementary Table S1 (DOCX 16 kb)

Supplemental Figure S1.

Overview of the host choice test of the BPH. (a) Overview of the host choice test before the BPH treatment. (b) Observation of the BPH location in NIL-9311 and 9311 plants at 120 h in the host choice test. The labels with ‘NIL’ and ‘AA11-6-1’ denote NIL-9311 plants; the labels with ‘9311’ and ‘P15’ denote 9311 plants (TIFF 8100 kb)

Supplemental Figure S2.

High-resolution genetic map of the Bph6 gene on rice chromosome 4 and the molecular marker genotypes and phenotypes of part recombinants. (a) High-resolution genetic linkage map. The numbers below the linkage map show the genetic distances (in cM). (b) Molecular marker genotypes and phenotypes of the recombinants. The black, white and gray bars denote the marker genotypes of Swarnalata homozygotes, 9311 homozygotes and heterozygotes, respectively. C1, control 1, homozygous for Swarnalata in the target region; C2, control 2, homozygous for 9311 in the target region; 9311, the susceptible parent; Swa, the resistant parent Swarnalata. a) BPH resistance score (mean ± SD, n = 7 replicates) (TIFF 5855 kb)

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Qiu, Y., Guo, J., Jing, S. et al. High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor Appl Genet 121, 1601–1611 (2010). https://doi.org/10.1007/s00122-010-1413-7

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