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Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t)

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Abstract

Genetic analysis and fine mapping of a resistance gene against brown planthopper (BPH) biotype 2 in rice was performed using two F2 populations derived from two crosses between a resistant indica cultivar (cv.), AS20-1, and two susceptible japonica cvs., Aichi Asahi and Lijiangxintuanheigu. Insect resistance was evaluated using F1 plants and the two F2 populations. The results showed that a single recessive gene, tentatively designated as bph19(t), conditioned the resistance in AS20-1. A linkage analysis, mainly employing microsatellite markers, was carried out in the two F2 populations through bulked segregant analysis and recessive class analysis (RCA), in combination with bioinformatics analysis (BIA). The resistance gene locus bph19(t) was finely mapped to a region of about 1.0 cM on the short arm of chromosome 3, flanked by markers RM6308 and RM3134, where one known marker RM1022, and four new markers, b1, b2, b3 and b4, developed in the present study were co-segregating with the locus. To physically map this locus, the bph19(t)-linked markers were landed on bacterial artificial chromosome or P1 artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. Sequence information of these clones was used to construct a physical map of the bph19(t) locus, in silico, by BIA. The bph19(t) locus was physically defined to an interval of about 60 kb. The detailed genetic and physical maps of the bph19(t) locus will facilitate marker-assisted gene pyramiding and cloning.

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Acknowledgements

We are grateful to F.-K. Huang at Guangxi Academy of Agricultural Sciences, China, for kindly providing the insect source and advice on BPH bioassay, and R.-Y. He for critical reading of the manuscript. This research was supported by grants from the National Basic Research Program of China (2006CB100200-G), the National Special Program for Functional Genomics and Biologic Chips (2002AA2E1002), the Guangdong Provincial Natural Science Foundation (000575; 039254), and the Special Project for the Distinguished University Professor from the Department of Education of Guangdong Province, China.

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  1. J. W. Chen

    Present address: Guangzhou Sugarcane Industry Research Institute, 510316, Guangzhou, China

Authors and Affiliations

  1. Laboratory of Plant Resistance and Genetics, College of Natural Resources and Environment, South China Agricultural University, 510642, Guangzhou, China

    J. W. Chen, L. Wang & Q. H. Pan

  2. Department of Entomology, College of Natural Resources and Environment, South China Agricultural University, 510642, Guangzhou, China

    J. W. Chen & X. F. Pang

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  1. J. W. Chen
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  2. L. Wang
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Correspondence to Q. H. Pan.

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Chen, J.W., Wang, L., Pang, X.F. et al. Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t). Mol Genet Genomics 275, 321–329 (2006). https://doi.org/10.1007/s00438-005-0088-2

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