Skip to main content
SpringerLink
Log in

High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Brown planthopper (BPH) is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. A molecular marker-based genetic analysis of BPH resistance was conducted using an F2 population derived from a cross between an introgression line, ‘IR71033-121-15’, from Oryza minuta (Accession number 101141) and a susceptible Korean japonica variety, ‘Junambyeo’. Resistance to BPH (biotype 1) was evaluated using 190 F3 families. Two major quantitative trait loci (QTLs) and two significant digenic epistatic interactions between marker intervals were identified for BPH resistance. One QTL was mapped to 193.4-kb region located on the short arm of chromosome 4, and the other QTL was mapped to a 194.0-kb region on the long arm of chromosome 12. The two QTLs additively increased the resistance to BPH. Markers co-segregating with the two resistance QTLs were developed at each locus. Comparing the physical map positions of the two QTLs with previously reported BPH resistance genes, we conclude that these major QTLs are new BPH resistance loci and have designated them as Bph20(t) on chromosome 4 and Bph21(t) on chromosome 12. This is the first report of BPH resistance genes from the wild species O. minuta. These two new genes and markers reported here will be useful to rice breeding programs interested in new sources of BPH resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Alam SN, Cohen MB (1998) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a double-haploid rice population. Theor Appl Genet 97:1370–1379

    Article  CAS  Google Scholar 

  • Amante AD, Sitch LA, Nelson R, Dalmacio RD, Oliva NP, Aswidi H, Leung H (1998) Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice O. sativa. Theor Appl Genet 84:345–354

    Google Scholar 

  • Brar DS, Khush GS (1997) Alien introgression in rice. Plant Mol Biol 35:35–47

    Article  PubMed  CAS  Google Scholar 

  • Chelliah S, Bharathi M (1993) Biotypes of the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae)—host influenced biology and behavior. In: Ananthakrishnan TN, Raman A (eds) Chemical ecology of phytopathogous insects. International Science Publishers, New York, pp 133–148

    Google Scholar 

  • Chen JW, Wang L, Pang XF, Pan QH (2006) Genetic analysis and fine mapping of a rice brown planthopper (Nilaparbata lugens stal) resistance gene bph19(t). Mol Gen Genomics 275:321–329

    Article  CAS  Google Scholar 

  • Chin JH, Kim JH, Jiang W, Chu SH, Woo MO, Han L, Brar D, Koh HJ (2007) Identification of subspecies-specific STS markers and their association with segregation distortion in rice (Oryza sativa L.). J Crop Sci Biotech 10(3):175–184

    Google Scholar 

  • Feuer R (1976) Biotype 2 brown planthopper in the Philippines. Int Rice Res New 1(1):15

    Google Scholar 

  • Heinrichs EA (1979) Control of leafhopper and planthopper vectors of rice viruses. In: Moramorosch K, Arris KF (eds) Leafhopper vectors and planthopper disease agents. Academic Press, New York, pp 529–558

    Google Scholar 

  • Heinrichs EA, Aquino GB, Chelliah S (1982) Resurgence of Nilaparvata lugens (Stål) populations as influenced by method and timing of insecticide applications in lowland rice. Environ Entomol 11:78–84

    Google Scholar 

  • Hirabayashi H, Ogawa T (1995) RFLP Mapping of Bph-1 (Brown planthopper resistance gene) in rice. Breed Sci 45:369–371

    CAS  Google Scholar 

  • Hirabayashi H, Kaji R, Angeles ER, Ogawa T, Brar DS, Khush GS (1999) RFLP analysis of a new gene for resistance to brown planthopper derived from O. officinalis on rice chromosome 4. (abstract in Japanese). Breed Sci 48(Suppl 1):48

    Google Scholar 

  • Huang Z, He G, Shu L, Li X, Zhang Q (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934

    Article  CAS  Google Scholar 

  • Huynh NV, Nhung HT (1988) High virulence of new brown planthopper (BPH) populations in the Mekong Delta, Vietnam. Int Rice Res New 13(5):16

    Google Scholar 

  • Ikeda R (1985) Studies of the inheritance of resistance to the rice brown planthopper (Nilaparvata lugens Stål) and the breeding of resistance rice cultivar. Bull Natl Agric Res Cent 3:1–54 (In Japanese)

    Google Scholar 

  • Ikeda R, Vaughan DA (1991) The distribution of resistance genes to the brown planthopper in rice germplasm. RGN 8:1–3

    Google Scholar 

  • IRRI (1988) Standard evaluation system for rice. IRRI, Manila

    Google Scholar 

  • Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37:217–221

    Article  PubMed  CAS  Google Scholar 

  • Jairin J, Phengrat K, Teangdeerith S, Vanavichit A, Toojinda T (2007) Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Mol Breed 19:35–44

    Article  CAS  Google Scholar 

  • Jena KK, Pasalu IC, Rao YK, Varalaxmi Y, Krishnaiah K, Khush GS, Kochert G (2002) Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Euphytica 129:81–88

    Article  Google Scholar 

  • Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2006) High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet 112:288–297

    Article  PubMed  CAS  Google Scholar 

  • Jeon YH, Ahn SN, Choi HC, Hahn TR, Moon HP (1999) Identification of a RAPD marker linked to a brown planthopper resistance gene. Euphytica 107:23–28

    Article  CAS  Google Scholar 

  • Kawaguchi M, Murata K, Ishii T, Takumi S, Mori N, Nakamura C (2001) Assignment of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph4 to rice chromosome 6. Breed Sci 51:13–18

    Article  CAS  Google Scholar 

  • Khush GS (1979) Genetics and breeding for resistance to brown planthopper. In:   IRRI (ed) Brown planthopper: threat to rice production in Asia. IRRI, Los Banõs, Philippines, pp 321–322

    Google Scholar 

  • Khush GS (1984) Breeding rice for resistance to insects. Prot Ecol 7:147–165

    Google Scholar 

  • Khush GS, Karim AR, Angeles EG (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper biotype. J Genet 64(2):121–125

    Article  Google Scholar 

  • Kim SM, Sohn JK (2005) Identification of a rice gene (Bph1) conferring resistance to brown planthopper (Nilaparvata lugens Stål) using STS markers. Mol Cells 20(1):30–34

    PubMed  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Liu GQ, Yan H, Fu Q, Qian Q, Zhang ZT, Zhai WX, Zhu LH (2001) Mappping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin Sci Bull 46:738–742

    Google Scholar 

  • Medrano FG, Heinrichs EA (1985) Response of resistant rices to brown planthoppers (BPH) collected in Mindanao, Philippines. Int Rice Res New 10(6):14–15

    Google Scholar 

  • Mochida O, Oka IN, Dandi S, Harahap Z, Sutjipto P, Beachell HM (1977) IR26 found susceptible to the brown planthopper in North Sumatra, Indonesia. Int Rice Res New 2(5):10–11

    Google Scholar 

  • Murata K, Fujiwara M, Kaneda C, Takumi S, Mori N, Nakamura C (1998) RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line ‘Norin-PL4. Genes Genet Syst 73:359–364

    Article  CAS  Google Scholar 

  • Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29:245–250

    CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Panaud O, Chen X, McCouch SR (1996) Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol Gen Genet 252:597–607

    PubMed  CAS  Google Scholar 

  • Panda N and Khush GS (1995) Host plant resistance to insects. CAB International, Wallingford, 431 pp

  • Park YD, Song YH (1988) Studies on the distribution of the brown planthopper (Nilaparvata lugens Stal) biotypes migrated in the southern regions of Korea. Korean J Appl Entomol 27(2):63–67

    Google Scholar 

  • Park DS, Lee SK, Lee JH, Song MY, Song SY, Kwak DY, Yeo US, Jeon NS, Park SK, Yi G, Song YC, Nam MH, Ku YC, Jeon JS (2007) The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis. Theor Appl Genet 115:357–547

    Article  CAS  Google Scholar 

  • Pathak MD, Khush GS (1979) Studies of varietal resistance in rice to the brown planthopper at the International Rice Research Institute. In: IRRI (ed) Brown planthopper: threat to rice production in Asia. pp 285–301.IRRI, Los Banõs, Philippines

  • Pathak MD, Cheng CH, Fortuno ME (1969) Resistance to Nephotettix impictiveps and Nilaparvata lugens in varieties of rice. Nature 223:502–504

    Article  Google Scholar 

  • Qiao Y, Jiang W, Rahman ML, Chu SH, Piao R, Han L, Koh HJ (2008) Comparison of molecular linkage maps and QTLs for morphological traits in two reciprocal backcross populations of rice. Mol Cells 25(3):417–427

    PubMed  CAS  Google Scholar 

  • Rahman ML, Chu SH, Choi MS, Qiao YL, Jiang W, Piao R, Khanam S, Cho YI, Jeung JU, Jena KK, Koh HJ (2007) Identification of QTLs for some agronomic traits in rice using an introgression line from Oryaza minuta. Mol Cells 24(1):16–26

    PubMed  CAS  Google Scholar 

  • Ren X, Wang X, Yuan H, Weng Q, Zhu L, He G (2004) Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice. Plant Breed 123:342–348

    Article  CAS  Google Scholar 

  • Renganayaki K, Feitz AK, Sadasivam S, Pammi S, Harrington SE, McCouch SR, Kumar SM, Reddy AS (2002) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. Crop Sci 42:2112–2117

    Article  CAS  Google Scholar 

  • Rivera CT, Ou SH, Lida TT (1966) Grassy stunt disease of rice and its transmission by Nilaparvata lugens (Stal). Plant Dis Rep 50:453–456

    Google Scholar 

  • Sharma PN, Ketipearachchi Y, Murata K, Torii A, Takumi S, Mori N, Nakamura C (2003) RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice. Euphytica 129(1):109–117

    Article  CAS  Google Scholar 

  • Sharma PN, Torii A, Takumi S, Mori N, Nakamura C (2004) Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stål) resistance genes Bph1 and Bph2 on rice chromosome 12. Heriditas 140:61–69

    Article  Google Scholar 

  • Sogawa K, Soekirno Raksadinata Y (1987) New genetic makeup of brown planthopper (BPH) populations in Central Java, Indonesia. Int Rice Res New 12(6):29–30

    Google Scholar 

  • Soundararajan RP, Kadirvel P, Gunathilagaraj K, Maheswaran M (2004) Mapping of Quantitative trait loci associated with resistance to brown planthopper in rice by means of a double haploid population. Crop Sci 44:2214–2220

    CAS  Google Scholar 

  • Stapley JH, May-Jackson YY, Golden WG (1979) Varietal resistance to the brown planthopper in the Solomon Islands. In: International Rice Research Institute (ed) Brown planthopper: threat to rice production in Asia. International Rice Research Institute, Los Banõs, pp 233–239

  • Su CC, Cheng XN, Zhai HQ, Wan JM (2002) Detection and analysis of QTL for resistance to brown planthopper, Nilaparvata lugens (Stål), in rice (Oryza sativa L.), using backcross inbred lines. Acta Genetica Sinica 29:332–338

    PubMed  Google Scholar 

  • Su CC, Wan J, Zhai HQ, Wang CM, Sun LH, Yasui H, Yoshimura A (2005) A new locus for resistance to brown planthopper identified in the indica rice variety DV85. Plant Breed 124:93–95

    Article  CAS  Google Scholar 

  • Su CC, Zhai HQ, Wang CM, Sun LH, Wan JM (2006) SSR mapping of brown planthopper resistance gene Bph9 in Kaharamana, an indica rice (Oryza sativa L.). Acta Genetica Sinica 33:262–268

    Article  PubMed  CAS  Google Scholar 

  • Sun L, Su C, Wang C, Zhai H, Wan J (2005) Mapping of a major resistance gene to the brown plant hopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396

    Article  CAS  Google Scholar 

  • Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTL × environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264

    Article  Google Scholar 

  • Wu JT, Heinrichs EA, Medrano FG (1986) Resistance of wild rice, Oryza spp., to the brown planthopper, Nilaparvata lugens (homoptera: Delphacidae). Environ Entomol 15:648–653

    Google Scholar 

  • Xu XF, Mei HW, Luo LJ, Cheng XN, Li ZK (2002) RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet 104:248–253

    Article  PubMed  CAS  Google Scholar 

  • Yang H, Ren X, Weng Q, Zhu L (2002) Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene. Hereditas 136:39–43

    Article  PubMed  Google Scholar 

  • Yang H, You A, Yang Z, Zhang F, He R, Zhu L, He G (2004) High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110:182–191

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by a grant (code# CG3111) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea. We are also thankful to Rural Development Administration, Korea for providing necessary greenhouse facilities for BPH bioassay.

Author information

Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Md Lutfor Rahman, Wenzhu Jiang, Sang Ho Chu, Yongli Qiao, Tae-Ho Ham, Mi-Ok Woo, Joohyun Lee, M. Sakina Khanam & Hee-Jong Koh

  2. Department of Crop Physiology, Bangladesh Institute of Nuclear Agriculture, Mymensingh, 2200, Bangladesh

    M. Sakina Khanam

  3. Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, DAPO BOX 7777, Manila, Laguna, Philippines

    Joong-Hyoun Chin & D. S. Brar

  4. Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, C/o IRRI-Korea Office, National Institute of Crop Science, RDA, Suwon, 441-857, Korea

    K. K. Jena

  5. Rice Research Division, National Institute of Crop Science, RDA, Suwon, 441-857, Korea

    Ji-Ung Jeung

Authors
  1. Md Lutfor Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenzhu Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sang Ho Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yongli Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tae-Ho Ham
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mi-Ok Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Joohyun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. Sakina Khanam
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Joong-Hyoun Chin
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ji-Ung Jeung
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. D. S. Brar
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. K. K. Jena
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Hee-Jong Koh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hee-Jong Koh.

Additional information

Communicated by T. Tai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rahman, M.L., Jiang, W., Chu, S.H. et al. High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta . Theor Appl Genet 119, 1237–1246 (2009). https://doi.org/10.1007/s00122-009-1125-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-009-1125-z

Keywords

Search

Navigation