Abstract
Brown planthopper (BPH), Nilaparvata lugens Stål, is a destructive insect pest of rice (Oryza sativa L.). Identification and utilization of resistance genes is an efficient strategy for controlling this insect. BPH-resistant indica cultivars Balamawee, Kaharamana and Pokkali were previously reported to have the same dominant gene Bph9 on chromosome 12. Our studies of BPH feeding performance showed that Balamawee had higher levels of antixenosis and antibiosis against BPH than Kaharamana and Pokkali. In order to identify the BPH resistance gene in Balamawee, an F2 population was derived by crossing Balamawee and susceptible japonica cultivar 02428. A single major resistance gene was identified and mapped to the long arm of chromosome 4. Further recombination analysis showed that the gene was located in an interval of about 63 kb between InDel markers Q52 and Q20. This new BPH resistance locus was designated Bph27(t).
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Acknowledgments
This research was supported by NSFC (31000697), a project from the Ministry of Agriculture of China for Transgenic Research (2011ZX08001-001), the PhD Programs Foundation of Ministry of Education of China (20100097120036), Natural Science Foundation of Jiangsu Province of China (BK2010442, BK2010016), and Jiangsu Science and Technology Development Program (BE2009301-3).
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Jun He and Yuqiang Liu contributed equally to this work.
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He, J., Liu, Y., Liu, Y. et al. High-resolution mapping of brown planthopper (BPH) resistance gene Bph27(t) in rice (Oryza sativa L.). Mol Breeding 31, 549–557 (2013). https://doi.org/10.1007/s11032-012-9814-8
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DOI: https://doi.org/10.1007/s11032-012-9814-8