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Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6

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Abstract

The brown planthopper (BPH) is one of the most destructive insect pests of rice in Thailand. We performed a cluster analysis that revealed the existence of four groups corresponding to the variation of virulence against BPH resistance genes in 45 BPH populations collected in Thailand. Rice cultivars Rathu Heenati and PTB33, which carry Bph3, showed a broad-spectrum resistance against all BPH populations used in this study. The resistant gene Bph3 has been extensively studied and used in rice breeding programs against BPH; however, the chromosomal location of Bph3 in the rice genome has not yet been determined. In this study, a simple sequence repeat (SSR) analysis was performed to identify and localize the Bph3 gene derived from cvs. Rathu Heenati and PTB33. For mapping of the Bph3 locus, we developed two backcross populations, BC1F2 and BC3F2, from crosses of PTB33 × RD6 and Rathu Heenati × KDML105, respectively, and evaluated these for BPH resistance. Thirty-six polymorphic SSR markers on chromosomes 4, 6 and 10 were used to survey 15 resistant (R) and 15 susceptible (S) individuals from the backcross populations. One SSR marker, RM190, on chromosome 6 was associated with resistance and susceptibility in both backcross populations. Additional SSR markers surrounding the RM190 locus were also examined to define the location of Bph3. Based on the linkage analysis of 208 BC1F2 and 333 BC3F2 individuals, we were able to map the Bph3 locus between two flanking SSR markers, RM589 and RM588, on the short arm of chromosome 6 within 0.9 and 1.4 cM, respectively. This study confirms both the location of Bph3 and the allelic relationship between Bph3 and bph4 on chromosome 6 that have been previously reported. The tightly linked SSR markers will facilitate marker-assisted gene pyramiding and provide the basis for map-based cloning of the resistant gene.

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References

  • Alam SN, Cohen MB (1998) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theor Appl Genet 97:1370–1379

    Article  CAS  Google Scholar 

  • Angeles ER, Khush GS, Heinrichs EA (1986) Inheritance of resistance to planthoppers and leafhopper in rice. In: IRRI (ed) Rice genetics. IRRI, Los Baños, Philippines, pp 537–549

  • Athwal DS, Pathak MD, Bacalangco EH, Pura CD (1971) Genetics of resistance to brown planthopper and green leafhopper in Oryza sativa L. Crop Sci 11:747–750

    Article  Google Scholar 

  • Chen DH, Ronald PC (1999) A rapid DNA minipreparation method suitable for AFLP and others PCR applications. Plant Mol Biol Rep 17:53–57

    Article  CAS  Google Scholar 

  • Chen JW, Wang L, Pang XF, Pan QH (2006) Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t). Mol Genet Genomics 275:321–329

    Article  PubMed  CAS  Google Scholar 

  • Heinrichs EA (1979) Control of leafhopper and planthopper vectors of rice viruses. In: Moramorosch K, Arris KF (eds) Leafhopper vectors and planthopper disease agents. Academic Press, New York, pp 529–558

    Google Scholar 

  • Heinrichs EA (1986) Perspectives and directions for the continued development of insect resistant rice varieties. Agric Ecosyst Environ 18:9–36

    Article  Google Scholar 

  • Heinrichs EA, Medrano FG, Rapusas HR (1985) Genetic evaluation for insect resistance in rice. IRRI, Los Baños, Philippines

    Google Scholar 

  • Huang N, Parco A, Mew T, Magpantay G, McCouch S, Guiderdoni E, Xu J, Subudhi P, Angeles ER, Khush GS (1997) RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Mol Breed 3:105–113

    Article  CAS  Google Scholar 

  • Huang Z, Shu L, Li X, Zhang Q (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934

    Article  CAS  Google Scholar 

  • Ikeda R (1985) Studies on the inheritance of resistance to the rice brown planthopper (Nilaparvata lugens Stål) and the breeding of resistance rice cultivars. Bull Natl Agric Res Cent 3:1–54

    Google Scholar 

  • Ikeda R, Kaneda C (1981) Genetic analysis of resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Jpn J Breed 31:279–285

    Google Scholar 

  • International Rice Research Institute (1996) Standard evaluation system for rice. Los Baños, Philippines

    Google Scholar 

  • Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37:217–221

    CAS  PubMed  Google Scholar 

  • Jairin J, Toojinda T, Tragoonrung S, Tayapat S, Vanavichit A (2005) Multiple genes determining brown planthopper (Nilaparvata lugens Stål) resistance in backcross introgressed lines of Thai jasmine rice ‘KDML105’. Sci Asia 31:129–135

    Article  CAS  Google Scholar 

  • Jena KK, Pasalu IC, Rao YK, Varalaxmi Y, Krishnaiah K, Khush GS and Kochert G (2003) Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L). Euphytica 129:81–88

    Article  CAS  Google Scholar 

  • Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2006) High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L). Theor Appl Genet 112:288–297

    Article  PubMed  CAS  Google Scholar 

  • Jeon YH, Ahn SN, Choi HC, Hahn TR, Moon HP (1999) Identification of a RAPD marker linked to a brown planthopper resistance gene in rice. Euphytica 107:23–28

    Article  CAS  Google Scholar 

  • Kabir MA, Khush GS (1988) Genetic analysis of resistance to brown planthopper in rice (O. sativa L). Plant Breed 100:54–58

    Article  Google Scholar 

  • Kawaguchi M, Mulata K, Ishii T, Takumi S, Mori N, Nakamura C (2001) Assignment of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph4 to the rice chromosome 6. Breed Sci 51:13–18

    Article  CAS  Google Scholar 

  • Khush GS (1984) Breeding rice for resistance to insects. Protect Ecol 7:147–165

    Google Scholar 

  • Khush GS, Rezaul ANM, Karim E, Angeles R (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper in rice (O sativa L). Plant Breed 100:54–58

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lakshminarayana A, Khush GS (1977) New genes for resistance to the brown planthopper in rice. Crop Sci 17:96–100

    Article  Google Scholar 

  • Li RB, Qin XY, Wei SM, Huang FK, Li Q, Luo SY (2002) Identification and genetics of resistance against brown planthopper in a derivative of wild rice, Oryza rufipogon Griff. J Genet Breed 56:29–36

    CAS  Google Scholar 

  • Liu GQ, Yan HH, Fu Q, Qian Q, Zhang ZT, Zhai WX, Zhu LH (2001) Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin Sci Bull 46:1459–1462

    Article  CAS  Google Scholar 

  • McCouch SR, Chen X, Panaid O, Temnykh S, Xu Y, Cho YG, Huang N, Ishii T, Blair M (1997) Microsatellite marker development, mapping and application in rice genetics and breeding. Plant Mol Biol 35:89–99

    Article  PubMed  CAS  Google Scholar 

  • McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  PubMed  CAS  Google Scholar 

  • Mei M, Zhuang C, Wan R, Wu J, Kochert G (1996) Genetic analysis and tagging of gene for brown planthopper resistant in indica rice. In: IRRI (ed) Rice Genetics III: Proc 3rd Int Rice Genet Symp. IRRI, Los Baños, Philippines, pp 590–595

  • Murai H, Hashimoto Z, Sharma PN, Shimizu T, Murata K, Takumi S, Mori N, Kawasaki S, Nakamura C (2001) Construction of a high-resolution linkage map of rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2. Theor Appl Genet 103:526–532

    Article  CAS  Google Scholar 

  • Murata K, Fujiwara M, Kaneda C, Takumi S, Mori N, Nakamura C (1998) RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line ‘Norin-PL4’. Genes Genet Syst 73:359–364

    Article  CAS  Google Scholar 

  • Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29:245–250

    CAS  Google Scholar 

  • Nemoto H, Ikeda R, Kaneda C (1989) New genes for resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Jpn J Breed 39:23–28

    CAS  Google Scholar 

  • Renganayaki K, Fritz AK, Sadasivam S, Pammi S, Harrington SE, McCouch SR, Kumar SM, Reddy AS (2002) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. Crop Sci 42:2112–2117

    Article  CAS  Google Scholar 

  • Sharma PN, Murata K, Torii A, Takumi S, Mori N, Nakamura C (2003a) Towards molecular cloning of resistance genes against brown planthopper (Nilaparvata lugens Stål) in rice: a case study of natural insect resistance genes. Trends Entomol 3:87–96

    Google Scholar 

  • Sharma PN, Ketipearachchi Y, Murata K, Torii A, Takumi S, Mori N, Nakamura C (2003b) RFLP/AFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph1 in rice. Euphytica 129:109–117

    Article  CAS  Google Scholar 

  • Sidhu GS, Khush GS (1979) Linkage relationships of some genes for disease and insect resistance and semidwarf stature in rice. Euphytica 28:233–237

    Article  Google Scholar 

  • Soundararajan RP, Kadirvel P, Gunathilagaraj K, Maheswaran M (2004) Mapping of quantitative trait loci associated with resistance to brown planthopper in rice by means of a doubled haploid population. Crop Sci 44:2214–2220

    Article  CAS  Google Scholar 

  • Su CC, Zhai HQ, Cheng XN, Wan JM (2002) Detection and analysis of QTLs for resistance to brown planthopper, Nilaparvata lugens (Stål), in rice (Oryza sativa L), using backcross in bred lines. Acta Genet Sin 29:332–338

    PubMed  Google Scholar 

  • Sun L, Su C, Wang C, Zhai H, Wan J (2005) Mapping of a major resistance gene to the brown planthopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396

    Article  CAS  Google Scholar 

  • Van Ooijen JW (2004) MapQTL 5, Software for the mapping of quantitative trait loci in experimental populations. Kyazma BV, Wageningen, The Netherlands

    Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap 30, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands

    Google Scholar 

  • Velusamy R, Heinrichs EA, Medrano FG (1986) Greenhouse techniques to identify field resistance to the brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidea), in rice cultivars. Crop Prot 5:328–333

    Article  Google Scholar 

  • Velusamy R, Ganesh Kumar M, Johnson Thangaraj Edward YS (1995) Mechanisms of resistance to brown planthopper Nilaparvata lugens in wild rice (Oryza spp) cultivars. Entomol Exp Appl 74:245–251

    Article  Google Scholar 

  • Watanabe T, Kitagawa H (2000) Photosynthesis and translocation of assimilates in rice plants following phloem feeding by the planthopper Nilaparvata lugens (Homoptera: Delphacidae). J Econ Entomol 93:1192–1198

    Article  PubMed  CAS  Google Scholar 

  • Wang B, Huang Z, Shu L, Ren X, Li X, He G (2001) Mapping of two new brown planthopper resistance genes from wild rice. Chin Sci Bull 46:1092–1095

    Article  CAS  Google Scholar 

  • Xu XF, Mei HW, Luo LJ, Cheng XN, Li ZK (2002) RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor Appl Genet 104:248–253

    Article  PubMed  CAS  Google Scholar 

  • Yan HM, Qin R, Jin WW, He GC, Song YC (2002) Comparative physical mapping of Bph3 with BAC-FISH in Oryza officinalis and O. sativa. Acta Bot Sin 44:583–587

    CAS  Google Scholar 

  • Yang H, You A, Yang Z, Zhang F, He R, Zhu L, He G (2004) High-resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110:182–191

    Google Scholar 

  • Yang H, Ren X, Weng Q, Zhu L, He G (2002) Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene. Hereditas 136:39–43

    Article  PubMed  Google Scholar 

  • Yuan H, Chen X, Zhu L, He G (2005) Identification of genes responsive to brown planthopper Nilaparvata lugens Stål (Homoptera: Delphacidae) feeding in rice. Planta 221:105–112

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We are grateful to The International Rice Research Institute, Philippines, for providing all seeds of BPH resistance cultivars. This research was conducted at Ubon Ratchathani Rice Research Center and Center of Excellence for Rice Molecular Breeding and Product Development, Thailand, and was partially supported by grant from the Rockefeller Foundation.

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  1. Ubon Ratchathani Rice Research Center, P.O. Box 65, Muang, Ubon Ratchathani, 34000, Thailand

    Jirapong Jairin, Kittiphong Phengrat & Sanguan Teangdeerith

  2. Center of Excellence for Rice Molecular Breeding and Product Development, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand

    Apichart Vanavichit & Theerayut Toojinda

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  1. Jirapong Jairin
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  2. Kittiphong Phengrat
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Correspondence to Jirapong Jairin.

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Jairin, J., Phengrat, K., Teangdeerith, S. et al. Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Mol Breeding 19, 35–44 (2007). https://doi.org/10.1007/s11032-006-9040-3

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