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QTL mapping integrated with BSA-Seq analysis identifies a novel gene conferring resistance to brown planthopper from common wild rice (Oryza rufipogon Griff.)

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Abstract

The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive rice pests worldwide. GXU202 is a germplasm of common wild rice (Oryza rufipogon Griff.) with high resistance to the BPH. In this study, genetic analysis indicated that the BPH-resistant phenotype of GXU202 is controlled by a major gene. Through the combination and comparison of QTL linkage and BSA-seq analyses, a novel gene locus, BPH41, conferring BPH resistance was identified. This gene locus was finely mapped to a 116-kb region delimited by W4-4–3 and W1-6–3 on chromosome 4. The markers D01031 and D01045 showed high accuracy in predicting phenotypes resistant to BPH, suggesting their reliability for marker-assisted selection of BPH41 in breeding BPH-resistant rice varieties. The present identification of BPH41 will establish a foundation for further map-based cloning and functional characterization of the gene.

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Funding

This research was funded by the National Natural Science Foundation of China (No. 31860416, No. 31460387) and the Science and Technology Project of Guangxi (No. GuikeAB16380093); Key projects of Guangxi Natural Science Foundation of China (Guangdong-Guangxi Joint Fund Project, No. 2021GXNSFDA075013).

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  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Agricultural College, Guangxi University, Nanning, 530004, China

    Xuan Wang, Yue Han, Xin-ying Guo, Yu-fen Qin, Yao-yu Fang, Fang Liu, Bao-xiang Qin & Rong-bai Li

  2. Guangxi Academy of Agricultural Science, Nanning, 530007, China

    Yue-xiong Zhang, Biao Deng & Bi-qiu Wu

  3. College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, 530005, China

    Ji-jing Luo

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  1. Xuan Wang
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Contributions

RL and JL designed and supervised the study. XW, YH, YZ, BD, BW, XG, YQ, YF, FL, and BQ performed the phenotypic data collection. XW analysed the data and drafted the manuscript. RL, JL and YZ revised and finalized the manuscript. All the authors read and approved the manuscript.

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Correspondence to Ji-jing Luo or Rong-bai Li.

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Wang, X., Han, Y., Zhang, Yx. et al. QTL mapping integrated with BSA-Seq analysis identifies a novel gene conferring resistance to brown planthopper from common wild rice (Oryza rufipogon Griff.). Euphytica 218, 34 (2022). https://doi.org/10.1007/s10681-021-02964-z

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