Skip to main content
SpringerLink
Log in

Fine mapping and characterization of BPH27, a brown planthopper resistance gene from wild rice (Oryza rufipogon Griff.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The brown planthopper (Nilaparvata lugens Stål; BPH) is one of the most serious rice pests worldwide. Growing resistant varieties is the most effective way to manage this insect, and wild rice species are a valuable source of resistance genes for developing resistant cultivars. BPH27 derived from an accession of Guangxi wild rice, Oryza rufipogon Griff. (Accession no. 2183, hereafter named GX2183), was primarily mapped to a 17-cM region on the long arm of the chromosome four. In this study, fine mapping of BPH27 was conducted using two BC1F2 populations derived from introgression lines of GX2183. Insect resistance was evaluated in the BC1F2 populations with 6,010 individual offsprings, and 346 resistance extremes were obtained and employed for fine mapping of BPH27. High-resolution linkage analysis defined the BPH27 locus to an 86.3-kb region in Nipponbare. Regarding the sequence information of rice cultivars, Nipponbare and 93-11, all predicted open reading frames (ORFs) in the fine-mapping region have been annotated as 11 types of proteins, and three ORFs encode disease-related proteins. Moreover, the average BPH numbers showed significant differences in 96–120 h after release in comparisons between the preliminary near-isogenic lines (pre-NILs, lines harboring resistance genes) and BaiR54. BPH growth and development were inhibited and survival rates were lower in the pre-NIL plants compared with the recurrent parent BaiR54. The pre-NIL exhibited 50.7 % reductions in population growth rates (PGR) compared to BaiR54. The new development in fine mapping of BPH27 will facilitate the efforts to clone this important resistant gene and to use it in BPH-resistance rice breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Alam SN, Cohen MB (1998a) Detection and analysis of QTLs for resistance to the brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theor Appl Genet 97:1370–1379

    Article  CAS  Google Scholar 

  • Alam SN, Cohen MB (1998b) Durability of brown planthopper, Nilaparvata lugens, resistance in rice variety IR64 in greenhouse selection studies. Entomol Exp Appl 89:71–78

    Article  CAS  Google Scholar 

  • Athwal DS, Pathak MD, Bacalangco EH, Pura CD (1971) Genetics of resistance to brown planthoppers and green leafhoppers in Oryza sativa L. Crop Sci 11:747–750

    Article  Google Scholar 

  • Cha YS, Ji H, Yun DW, Ahn BO, Lee MC, Suh SC, Lee CS, Ahn EK, Jeon YH, Jin ID, Sohn JK, Koh HJ, Eun MY (2008) Fine mapping of the rice Bph1 gene, which confers resistance to the brown planthopper (Nilaparvata lugens Stål), and development of STS markers for marker-assisted selection. Mol Cells 26(2):146–151

    PubMed  CAS  Google Scholar 

  • Chen JW, Wang L, Pang XF, Pan QH (2006) Genetic analysis and fine mapping of a rice brown planthopper (Nilaparbata lugens Stål) resistance gene bph19(t). Mol Genet Genomics 275:321–329

    Article  PubMed  CAS  Google Scholar 

  • Cohen MB, Alam SN, Medina EB, Bernal CC (1997) Brown planthopper, Nilaparvata lugens, resistance in rice cultivar IR64: mechanism and role in successful N. lugens management in Central Luzon, Philippines. Entomol Exp Appl 85:221–229

    Article  Google Scholar 

  • Daniel X, Lacomme C, Morel JB, Roby D (1999) A novel myb oncogene homolog in Arabidopsis thaliana related to the hypersensitive cell death. Plant J 20:57–66

    Article  PubMed  CAS  Google Scholar 

  • Du B, Zhang WL, Liu BF, Hu J, Wei Z, Shi ZY, He RF, Zhu LL, Chen RZ, Han B, He GC (2009) Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice. Proc Natl Acad Sci USA 106(52):22163–22168

    Article  PubMed  CAS  Google Scholar 

  • Edwards OR (2001) Interspecific and intraspecific variation in the performance of three pest aphid species on five grain legume hosts. Entomol Exp Appl 100:21–30

    Google Scholar 

  • Guzzo F, Portaluppi P, Grisi R, Barone S, Zampieri S, Franssen H, Levi M (2005) Reduction of cell size induced by enod40 in Arabidopsis thaliana. J Exp Bot 56(412):507–513

    Article  PubMed  CAS  Google Scholar 

  • Hirabayashi H, Ogawa T (1995) RFLP mapping of Bph-1 (brown planthopper resistance gene) in rice. Breed Sci 45:369–371

    CAS  Google Scholar 

  • Hirabayashi H, Angeles ER, Kaji R, Ogawa T, Brar DS, Khush GS (1998) Identification of brown planthopper resistance gene derived from O. officinalis using molecular markers in rice. Breed Sci 48(Suppl):82 (in Japanese)

    Google Scholar 

  • Hospital F, Chevalet C, Mulsant P (1992) Using markers in gene introgression breeding programs. Genetics 132:199–210

    Google Scholar 

  • Hu XB, Song FM, Zheng Z (2006) Molecular cloning and expression analysis of rice OsTVLP1, encoding a protein with similarity to TGF-receptor interacting proteins and vacuolar assembly Vam6p/Vps39p proteins. DNA Seq 17:152–158

    Article  PubMed  CAS  Google Scholar 

  • Huang Z, He GC, Shu LH, Li XH, Zhang QF (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934

    Article  CAS  Google Scholar 

  • IRRI (1988) Standard evaluation system for rice. International Rice Research Institute, Manila

    Google Scholar 

  • Ishii T, Brar DS, Multani DS, Khush GS (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from Oryza australiensis into cultivated rice, O. sativa. Genome 37:217–221

    Article  PubMed  CAS  Google Scholar 

  • Jairin J, Phengrat K, Teangdeerith S, Vanavichit A, Toojinda T (2007) Mapping of a broad-spectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Mol Breed 19:35–44

    Article  CAS  Google Scholar 

  • Jena KK, Jeung JU, Lee JH, Choi HC, Brar DS (2006) High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.). Theor Appl Genet 112:288–297

    Article  PubMed  CAS  Google Scholar 

  • Kabis MA, Khush GS (1988) Genetic analysis of resistance to brown planthopper in rice, Oryza sativa L. Plant Breed 100:54–58

    Article  Google Scholar 

  • Kawaguchi M, Murata K, Ishii T, Takumi S, Mori N, Nakamura C (2001) Assignment of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph4 to rice chromosome 6. Breed Sci 51:13–18

    Article  CAS  Google Scholar 

  • Khush GS, Karim AR, Angeles EG (1985) Genetics of resistance of rice cultivar ARC10550 to Bangladesh brown planthopper biotype. J Genet 64(2):121–125

    Article  Google Scholar 

  • Klingler J, Creasy R, Gao LL, Nair RM, Calix AS, Jacob HS, Edwards OR, Singh KB (2005) Aphid resistance in Medicago truncatula involves antixenosis and phloem-specific, inducible antibiosis, and maps to a single locus flanked by NBS-LRR resistance gene analogs. Plant Physiol 137:1445–1455

    Article  PubMed  CAS  Google Scholar 

  • Lakshminarayana A, Khush GS (1977) New genes for resistance to the brown planthopper in rice. Crop Sci 17:96–100

    Article  Google Scholar 

  • Li RB, Li LS, Wei SM, Wei YP, Chen YZ, Bai DL, Yang L, Huang FK, Lv WL, Zhang XJ, Li XY, Yang XQ, Wei YW (2006) The evaluation and utilization of new genes for brown planthopper resistance in common wild rice (Oryza rufipogon Griff.). Mol Plant Breed 4(3):365–371

    CAS  Google Scholar 

  • Li RB, Chen YZ, Wei YP, Liu C, Ma ZF, Huang DH, Zhang YX, Lv WL (2009) Mapping of a wide compatibility locus in indica rice using SSR markers. Mol Breed 24(2):135–140

    Article  CAS  Google Scholar 

  • Ling KC, Tiongco ER, Aguiero VM (1977) Transmission of rice ragged stunt disease. Int Rice Res Newsl 2(6):11–12

    Google Scholar 

  • Liu GQ, Yan H, Fu Q, Qian Q, Zhang ZT, Zhai WX, Zhu LH (2001) Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin Sci Bull 46:738–742

    Google Scholar 

  • Luo JJ, Hao W, Jin J, Gao JP, Lin HX (2008) Fine mapping of Spr3, a locus for spreading panicle from African cultivated rice (Oryza glaberrima Steud.). Mol Plant 1:830–838

    Article  PubMed  Google Scholar 

  • Massague J (1998) TGF-beta signal transduction. Annu Rev Biochem 67:753–791

    Article  PubMed  CAS  Google Scholar 

  • McCouch SR, CGSNL (2008) Gene nomenclature system for rice. Rice 1:72–84

  • Murai H, Hashimoto Z, Sharma PN, Shimizu T, Murata K, Takumi S, Mori N, Kawasaki S, Nakamura C (2001) Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2. Theor Appl Genet 103:526–532

    Article  CAS  Google Scholar 

  • Murata K, Fujiwara M, Kaneda C, Takumi S, Mori N, Nakamura C (1998) RFLP mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene bph2 of indica rice introgressed into a japonica breeding line ‘Norin-PL4’. Genes Genet Syst 73:359–364

    Article  CAS  Google Scholar 

  • Murata K, Fujiwara M, Murai H, Takumi S, Mori N, Nakamura C (2001) Mapping of a brown planthopper (Nilaparvata lugens Stål) resistance gene Bph9 on the long arm of rice chromosome 12. Cereal Res Commun 29:245–250

    CAS  Google Scholar 

  • Murray MG, Thompson WK (1980) Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Myint KKM, Fujita T, Matsumura M, Sonoda T, Yoshimura A, Yasui H (2012) Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stål]) in the rice cultivar ADR52. Theor Appl Genet 124:495–504

    Article  PubMed  CAS  Google Scholar 

  • Nemoto H, Ikeda R, Kaneda C (1989) New genes for resistance to brown planthopper, Nilaparvata lugens Stål, in rice. Jpn J Breed 39:23–28

    CAS  Google Scholar 

  • Normile D (2008) Reinventing rice to feed the world. Science 321:330–333

    Article  PubMed  CAS  Google Scholar 

  • Painter RH (1951) Insect resistance in crop plants. The Macmillan Company, New York, p 520

    Google Scholar 

  • Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2010) High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor Appl Genet 121:1601–1611

    Article  PubMed  Google Scholar 

  • Qiu YF, Guo JP, Jing SL, Tang M, Zhu LL, He GC (2011) Identification of antibiosis and tolerance in rice varieties carrying brown planthopper resistance genes. Entomol Exp Appl 141:224–231

    Article  CAS  Google Scholar 

  • Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2012) Development and characterization of japonica rice lines carrying the brown planthopper resistance genes BPH12 and BPH6. Theor Appl Genet 124(3):485–494

    Article  PubMed  CAS  Google Scholar 

  • Raffaele S, Rivas S, Roby D (2006) An essential role for salicylic acid in AtMYB30-mediated control of the hypersensitive cell death program in Arabidopsis. FEBS Lett 580:3498–3504

    Article  PubMed  CAS  Google Scholar 

  • Rahman ML, Jiang WZ, Chu SH, Qiao YL, Ham TH, Woo MO, Lee JH, Khanam MS, Chin JH, Jeung JU, Brar DS, Jena KK, Koh HJ (2009) High-resolution mapping of two rice brown planthopper resistance genes, Bph20(t) and Bph21(t), originating from Oryza minuta. Theor Appl Genet 119(7):1237–1246

    Article  PubMed  Google Scholar 

  • Renganayaki K, Feitz AK, Sadasivam S, Pammi S, Harrington SE, McCouch SR, Kumar SM, Reddy AS (2002) Mapping and progress toward map-based cloning of brown planthopper biotype-4 resistance gene introgressed from Oryza officinalis into cultivated rice, O. sativa. Crop Sci 42:2112–2117

    Article  CAS  Google Scholar 

  • Rivera CT, Ou SH, Lida TT (1966) Grassy stunt disease of rice and its transmission by Nilaparvata lugens (Stål). Plant Dis Rep 50:453–456

    Google Scholar 

  • Rivers RL, Dean RM, Chandy G, Hall JE, Roberts DM, Zeidel ML (1997) Functional analysis of nodulin 26, an aquaporin in soybean root nodule symbiosomes. J Biol Chem 272:16256–16261

    Article  PubMed  CAS  Google Scholar 

  • Seo PJ, Park CM (2010) MYB96-mediated abscisic acid signals induce pathogen resistance response by promoting salicylic acid biosynthesis in Arabidopsis. New Phytol 186(2):471–483

    Article  PubMed  CAS  Google Scholar 

  • Sogawa K (1982) The rice brown planthopper: feeding physiology and host plant interactions. Annu Rev Entomol 27:49–73

    Article  CAS  Google Scholar 

  • Sun LH, Su CC, Wang CM, Zhai HQ, Wan JM (2005) Mapping of a major resistance gene to the brown planthopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396

    Article  CAS  Google Scholar 

  • Vailleau F, Daniel X, Tronchet M, Montillet JL, Triantaphylides C, Roby D (2002) A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack. Proc Natl Acad Sci USA 99:10179–10184

    Article  PubMed  CAS  Google Scholar 

  • Wang YY, Wang XL, Yuan HY, Chen RZ, Zhu LL, He RF, He GC (2008) Responses of two contrasting genotypes of rice to brown planthopper. Mol Plant Microbe Interact 21:122–132

    Article  PubMed  CAS  Google Scholar 

  • Wilson RC, Long FX, Maruoka EM, Cooper JB (1994) A new proline-rich early nodulin from Medicago truncatula is highly expressed in nodule meristematic cells. Plant Cell 6:1256–1275

    Google Scholar 

  • Yamazaki Y, Tsuchiya R, Takahashi Y, Asanuma T, Shidahara Y, Sakaniwa S (2010) Rice genes in Oryza base. Nat Preced. doi:10.1038/npre.2010.5069.1

    Google Scholar 

  • Yang HY, Ren X, Weng QM, Zhu LL, He GC (2002) Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene. Hereditas 136:39–43

    Article  PubMed  Google Scholar 

  • Yang HY, You AQ, Yang ZF, Zhang FT, He RF, Zhu LL, He GC (2004) High resolution genetic mapping at the Bph15 locus for brown planthopper resistance in rice (Oryza sativa L.). Theor Appl Genet 110:182–191

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank C. Liu, Z. F. Ma, Y. P. Wei, L. S. Li and Y. Z. Chen for gene mapping and field work. This research was supported by the Major Program of National Science and Technology Project for Breeding of Transgenic Biological Varieties (2009ZX08009-047B), National High Technology Research and Development Program (863) (2007AA021405, 2007AA10Z191), Guangxi Natural Science Foundation (0833025) and Guangxi Science and Technology Projects (0815011-6-1, 09-007-08).

Author information

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Agricultural College, Guangxi University, Nanning, 530005, China

    D. Huang, Y. Qiu, J. Meng, R. Li & B. Chen

  2. Rice Research Institute, Plant Protection Institute and Guangxi Rice Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    D. Huang, Y. Zhang, F. Huang & S. Wei

Authors
  1. D. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. B. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to R. Li or B. Chen.

Additional information

Communicated by T. Sasaki.

D. Huang and Y. Qiu contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 25 kb)

122_2012_1975_MOESM2_ESM.tif

Supplementary Figure S1 BPH-resistance genes on rice chromosome 4. Mapping region of the resistance genes: BPH12 (formerly Bph12(t)) from O. latifolia, mapped between RM16459 and RM1305 (Qiu et al. 2012); Bph15 from O. officinalis, mapped between RG1 and RG2 (Yang et al. 2004); Bph17 from cv. Rathu Heenati, mapped between RM8213 and RM5953 (Sun et al, 2005); Bph20(t) from O. minuta, mapped between B42 and B44 (Rahman et al. 2009); bph16 from O. officinalis, mapped between G271 and R93 (Hirabayashi et al. 1998); BPH6 (formerly Bph6) from cv. Swarnalata, mapped between RM6997 and RM5742 (Qiu et al. 2010). BPH27 from O. rufipogon, mapped between RM16846 and RM16853 (TIFF 1728 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, D., Qiu, Y., Zhang, Y. et al. Fine mapping and characterization of BPH27, a brown planthopper resistance gene from wild rice (Oryza rufipogon Griff.). Theor Appl Genet 126, 219–229 (2013). https://doi.org/10.1007/s00122-012-1975-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-012-1975-7

Keywords

Search

Navigation