Abstract
Key message
A QTL associated with BPH resistance at the early seedling stage was identified on chromosome 3. Functional Bph14 in Rathu Heenati was associated with BPH resistance at the early seedling stage.
Abstract
Brown planthopper (BPH; Nilaparvata lugens Stål) is considered the most important rice pest in many Asian countries. Several BPH resistance genes have previously been identified. However, there are few reports of genes specific for BPH resistance at the early seedling stage, a crucial stage for direct-seeding cultivation. In this study, we performed a QTL-seq analysis using two bulks (20 F2 lines in each bulk) of the F2 population (n = 300) derived from a cross of Rathu Heenati (RH) × HCS-1 to identify QTL/genes associated with BPH resistance at the early seedling stage. An important QTL was identified on chromosome 3 and Bph14 was identified as a potential candidate gene based on the differences in gene expression and sequence variation when compared with the two parents. All plants in the resistant bulks possessed the functional Bph14 from RH and all plants in the susceptible bulk and HCS-1 contained a large deletion (2703 bp) in Bph14. The functional Bph14 gene of RH appears to be important for BPH resistance at the early seedling stage of rice and could be used in conjunction with other BPH resistance genes in rice breeding programs that confer resistance to BPH at the early and later growth stages.
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Data availability
The RNA-seq data described herein have been deposited in the NCBI repository, Accession Number PRJNA862304.
Change history
07 April 2023
A Correction to this paper has been published: https://doi.org/10.1007/s00122-023-04338-6
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Acknowledgements
We thank the Rice Department, the Ministry of Agriculture and Cooperatives, and the Rice Science Center for providing the plant materials used in this study. We also thank the Innovative Plant Biotechnology and Precision Agriculture Research Team, National Center for Genetic Engineering and Biotechnology, Thailand, for providing the SNP data. We also thank Joanna Friesner, Donald Danforth Plant Science Center, for proofreading and insightful comments while reviewing the manuscript.
Funding
This work was financially supported by the National Research Council of Thailand (NRCT Grant Number: NRCT-RTA/812/2563), the National Science and Technology Development Agency, Thailand (NSTDA Grant Number: P-12-01475 and P-18-52853), the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021. The research was also supported by the Center of Excellence on Agricultural Biotechnology, Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation. (AG‐BIO/MHESI) with Grant No. AG‐BIO/60-005-006.
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SA, TT and BCM conceived the study. SA, SW, MS, WJ designed the experiments. SP performed experiments. SP and WA analyzed data. SP, MKP and SW wrote the manuscript. SW, SA and BCM reviewed the manuscript. All authors read and approved the final manuscript.
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Pannak, S., Wanchana, S., Aesomnuk, W. et al. Functional Bph14 from Rathu Heenati promotes resistance to BPH at the early seedling stage of rice (Oryza sativa L.) as revealed by QTL-seq. Theor Appl Genet 136, 25 (2023). https://doi.org/10.1007/s00122-023-04318-w
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DOI: https://doi.org/10.1007/s00122-023-04318-w