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Genetic mapping of rust resistance genes in confection sunflower line HA-R6 and oilseed line RHA 397

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Abstract

Few widely effective resistance sources to sunflower rust, incited by Puccinia helianthi Schwein., have been identified in confection sunflower (Helianthus annuus L.). The USDA inbred line HA-R6 is one of the few confection sunflower lines resistant to rust. A previous allelism test indicated that rust resistance genes in HA-R6 and RHA 397, an oilseed-type restorer line, are either allelic or closely linked; however, neither have been characterized nor molecularly mapped. The objectives of this study are (1) to locate the rust resistance genes in HA-R6 and RHA 397 on a molecular map, (2) to develop closely linked molecular markers for rust resistance diagnostics, and (3) to determine the resistance spectrum of two lines when compared with other rust-resistant lines. Two populations of 140 F2:3 families each from the crosses of HA 89, as susceptible parent, with HA-R6 and RHA 397 were inoculated with race 336 of P. helianthi in the greenhouse. The resistance genes (R-genes) in HA-R6 and RHA 397 were molecularly mapped to the lower end of linkage group 13, which encompasses a large R-gene cluster, and were designated as R 13a and R 13b, respectively. In the initial maps, SSR (simple sequence repeat) and InDel (insertion and deletion) markers revealed 2.8 and 8.2 cM flanking regions for R 13a and R 13b, respectively, linked with a common marker set of four co-segregating markers, ORS191, ORS316, ORS581, and ZVG61, in the distal side and one marker ORS464 in the proximal side. To identify new markers closer to the genes, sunflower RGC (resistance gene candidate) markers linked to the downy mildew R-gene Pl 8 and located at the same region as R 13a and R 13b were selected to screen the two F2 populations. The RGC markers RGC15/16 and a newly developed marker SUN14 designed from a BAC contig anchored by RGC251 further narrowed down the region flanking R 13a and R 13b to 1.1 and 0.1 cM, respectively. Both R 13a and R 13b are highly effective against all rust races tested so far. Our newly developed molecular markers will facilitate breeding efforts to pyramid the R 13 genes with other rust R-genes and accelerate the development of rust-resistant sunflower hybrids in both confection and oilseed sunflowers.

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Acknowledgments

We thank Drs. Chao-Chien Jan and Zengcui Zhang for critical review of the manuscript, and Angelia Hogness for technical assistance. We also thank Andrew Friskop for providing some single-pustule isolates. This project was supported by the USDA-AMS Cooperative Agreement 12-25-B-1247.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58102, USA

    L. Gong & S. G. Markell

  2. USDA-Agricultural Research Service, Northern Crop Science Laboratory, 1605 Albrecht Blvd N, Fargo, ND, 58102-2765, USA

    T. J. Gulya, B. S. Hulke & L. L. Qi

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  1. L. Gong
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  2. T. J. Gulya
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  3. S. G. Markell
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  4. B. S. Hulke
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  5. L. L. Qi
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Correspondence to L. L. Qi.

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Communicated by A. Bervillé.

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Gong, L., Gulya, T.J., Markell, S.G. et al. Genetic mapping of rust resistance genes in confection sunflower line HA-R6 and oilseed line RHA 397. Theor Appl Genet 126, 2039–2049 (2013). https://doi.org/10.1007/s00122-013-2116-7

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