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Downy mildew (Pl 8 and Pl 14 ) and rust (R Adv ) resistance genes reside in close proximity to tandemly duplicated clusters of non-TIR-like NBS-LRR-encoding genes on sunflower chromosomes 1 and 13

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Abstract

Nucleotide binding site-leucine rich repeat (NBS-LRR) proteins are encoded by a ubiquitous gene family in sunflower and frequently harbor disease resistance genes. We investigated NBS-LRR-encoding resistance gene candidates (RGCs) flanking the downy mildew resistance genes Pl 8 and Pl 14 and the rust resistance gene R Adv , which map on the NBS-LRR clusters of linkage groups 1 and 13 in sunflower genome. We shotgun sequenced bacterial artificial chromosome (BAC) clones proximal to Pl 8 , Pl 14 , and R Adv and identified seven novel non-Toll/interleukin-1 receptor (TIR)-like NBS-LRR RGCs, which clustered with previously identified RGCs of linkage group 13 but were phylogenetically distant from the TIR- and non-TIR-NBS-LRR-encoding superfamilies of sunflower. Six of the seven predicted RGCs have intact open reading frames and reside in genomic segments with abundant transposable elements. The genomic localization and sequence similarity of the novel non-TIR-like predicted RGCs suggests that they originated from tandem duplications. RGCs in the proximity of Pl 8 and R Adv were likely introgressed from silverleaf sunflower genome, where the RGC cluster of linkage group 13 is duplicated in two independent chromosomes that have different architecture and level of recombination from the respective common sunflower chromosomes.

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Acknowledgments

This research was supported by grants from the United States Department of Agriculture Plant Genome Program (no. 2000-04292) and the National Science Foundation Plant Genome Program (no. 0421630).

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Authors and Affiliations

  1. Institute of Plant Breeding, Genetics and Genomics, 111 Riverbend Rd, The University of Georgia, Athens, GA, 30602, USA

    Eleni Bachlava, Osman E. Radwan, Shunxue Tang, Wenxiang Gao, Adam F. Heesacker & Steven J. Knapp

  2. Monsanto Company, 37437 State Hwy 16, Woodland, CA, 95695, USA

    Eleni Bachlava & Steven J. Knapp

  3. University of Illinois, 1401 West Green St, Urbana, IL, 61801, USA

    Osman E. Radwan

  4. Advanta Seeds, Balcarce Research Station Ruta 226, KM 60.3 (7620), Balcarce PCIA de Buenos Aires, Argentina

    Gustavo Abratti, Maria E. Bazzalo, Andres Zambelli & Alberto J. Leon

  5. Pioneer Hi-Bred, Ruta 178 KM 11.5 (2700), Pergamino, Buenos Aires, Argentina

    Gustavo Abratti

  6. Dow AgroSciences LLC, 9330 Zionsville Rd, 46268, Indianapolis, IN, USA

    Shunxue Tang & Wenxiang Gao

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  1. Eleni Bachlava
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  2. Osman E. Radwan
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  3. Gustavo Abratti
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  4. Shunxue Tang
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  5. Wenxiang Gao
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  6. Adam F. Heesacker
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  7. Maria E. Bazzalo
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  8. Andres Zambelli
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  9. Alberto J. Leon
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  10. Steven J. Knapp
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Correspondence to Eleni Bachlava.

Additional information

Communicated by T. Close.

Electronic supplementary material

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ESM 1

BAC-end sequences (BESs) of all positive BAC clones from contigs flanking Pl 8 , R Adv and Pl 14 genes, and their best BLAST hit, if applicable (PDF 13 kb)

ESM 2

RGCs of linkage groups 1 and 13 genotyped in IMISUN-1 × 29004 and ZENB8 x RHA340 populations. The table includes marker type, GenBank accession, forward and reverse primers for amplification of each RGC and the motif corresponding to the amplified region (PDF 59 kb)

ESM 3

Maximum likelihood tree including the 783 previously identified TIR- and non-TIR-NBS-LRR-encoding RGCs of sunflower (Radwan et al. 2008) and seven putative RGCs mined from P102A12, P408L01 and P339N08. One of the 100 maximum likelihood trees was randomly chosen to demonstrate the actual branch lengths corresponding to evolutionary distance that cannot be deduced from the consensus tree (PDF 74 kb)

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Bachlava, E., Radwan, O.E., Abratti, G. et al. Downy mildew (Pl 8 and Pl 14 ) and rust (R Adv ) resistance genes reside in close proximity to tandemly duplicated clusters of non-TIR-like NBS-LRR-encoding genes on sunflower chromosomes 1 and 13. Theor Appl Genet 122, 1211–1221 (2011). https://doi.org/10.1007/s00122-010-1525-0

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