Abstract
This paper provides the first description of a consensus map of the cultivated sunflower genome (Helianthus annuus L., n=17 chromosomes), based on RFLP. A total of 180 probe-enzyme combinations were mapped on at least one of five segregating progenies (three F2 and two BC1 populations), revealing 237 loci that did not show any distortion of segregation. The consensus linkage map obtained with these loci covers 1150 cM and consists of 16 linkage groups of more than 20 cM, 7 groups of less than 20 cM and 18 unlinked loci. The mean distance between loci is 7 cM, but in some regions intervals of 20 cM remain. Genotypic and gametic segregation distortions affect about 7% of loci. It was found that 25% of the probes mapped using several different restriction enzymes or that on different progenies they revealed 2 or more loci.
Similar content being viewed by others
References
Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rept 9:208–218
Bailey NTJ (1961) Introduction to the mathematical theory of genetic linkage. Clarendon Press, Oxford
Beavis WD, Grant D (1991) A linkage map based on information from four F2 populations of maize (Zea mays L.). Theor Appl Genet 82:636–644
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526
Choumane W, Heizmann P (1988) Structure and variability of nuclear ribosomal genes in the genus Helianthus. Theor Appl Genet 76:481–489
Chyi Y-S, Hoenecke ME, Sernyk JL (1992) A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn campestris). Genome 35:746–757
Diers BW, Keim P, Fehr WR, Shoemaker RC (1992) RFLP analysis of soybean seed protein and oil content. Theor Appl Genet 83:608–612
Elkharbotly A, Leonardsschippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C (1994) Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242:749
Gardiner JM, Coe EH, Melia-Hancock S, Hoisington DA, Chao S (1993) Development of a core RFLP map in maize using an immortalized F2 population. Genetics 134:917–930
Gebhardt RE, Ritter E, Debener T, Sachtschabel U, Walkenmeier B, Uhrig H, Salimini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gentzbittel L, Perrault A, Nicolas P (1992) Molecular phylogeny of the Helianthus genus, based on nuclear restriction-fragment-length polymorphism (RFLP). Mol Biol Evol 9:872–892
Gentzbittel L, Zhang Y-X, Year F, Griveau Y, Nicolas P (1994) RFLP studies of genetic relationships among inbred lines of cultivated sunflower, Helianthus annuus L.: evidence for distinct restorer and maintainer germplasm pools. Theor Appl Genet 89:419–425
Hartl L, Weiss H, Zeller FJ, Jahoor A (1993) Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.) Theor Appl Genet 86:959–963
Helentjaris T (1987) A genetic map for maize based on RFLPs. Trends Genet 3:217–221
Helentjaris T, Weber D, Wright S (1988) Identification of the genomic locations of duplicated sequences in maize by analysis of restriction fragment length polymorphisms. Genetics 118:353–363
Holloway JL, Knapp SJ (1993) GMendel 3.0 users guide, Department of Crop and Soil Science, Oregon State University, Corvallis, Ore., USA
Jackson RC, Murray BG (1983) Colchicine induced quadrivalent formation in Helianthus: evidence for ancient polyploidy. Theor Appl Genet 64:219–222
Kahler AL, Lay CL (1985) Genetics of electrophoretic variants in the annual sunflower. J Her 76:335–340
Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Laver HK, Reynolds SJ, Moneger F, Leaver CJ (1191) Mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower (Helianthus annuus) Plant J 1:185–193
Leclercq P (1966) Une stérilité mâle utilisable pour la production d'hybride simples de tournesol. Ann Amelior Plant 16:134–144
Leclercq P (1969) Une stérilité mâle cytoplasmique chez le Tournesol. Ann Amelior Plant 19:99–106
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into mendelian factors by using complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Putt ED (1964) Recessive branching in sunflowers. Crop Sci 4:444–445
Quillet MC, Vear F, Branlard G (1992) The use of isozyme polymorphism for identification of sunflower (Helianthus annuus) inbred lines. J Genet Breed 46:295–304
Rieseberg LH, Beckstrom-Sternberg S, Doan K (1990) Helianthus annuus ssp. texanus has chloroplast DNA and nuclear ribosomal RNA genes of Helianthus debilis ssp cucumerifolius. Proc Natl Acad Sci USA 87:593–597
Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa (syn campestris) based on restriction fragment length polymorphism. Theor Appl Genet 82:296–304
Tersac M, Blanchard P, Brunel D, Vincourt P (1994) Relations between heterosis and enzymatic polymorphism in populations of cultivated sunflowers (Helianthus annuus L.). Theor Appl Genet 88:49–55
Author information
Authors and Affiliations
Additional information
Communicated by J.W. Snape
Rights and permissions
About this article
Cite this article
Gentzbittel, L., Vear, F., Zhang, YX. et al. Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.). Theoret. Appl. Genetics 90, 1079–1086 (1995). https://doi.org/10.1007/BF00222925
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222925