Abstract
The Pl1 locus in sunflower, Helianthus annuus L., conferring resistance to downy mildew, Plasmopara halstedii, race 1 has been located in linkage group 1 of the consensus RFLP map of the cultivated sunflower. Bulked segregant analyses were used on 135 plants of an F2 progeny from a cross between a downy mildew susceptible line, GH, and RHA266, a line carrying Pl1. Two RFLP markers and one RAPD marker linked to the Pl1 locus have been identified. The RFLP markers are located at 5.6 cM and 7.1 cM on either side of Pl1. The RAPD marker is situated at 43.7 cM from Pl1. The significance and applications of these markers in sunflower breeding are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adam-Blondon AF, Sévignac M, Bannerot H, Dron M (1994) SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean. Theor Appl Genet 88:865–870
Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt R, Giraudat J, Leung J, Staskawicz BJ (1994) RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265:1856–1860
Fick GN, Zimmer DE (1974) RHA271, RHA273 and RHA274 sunflower parental lines for producing downy mildew-resistant hybrids. Farm Res 32:7–9
Gentzbittel L, Perrault A, Nicolas P (1992) Molecular phylogeny of the Helianthus genus, based on nuclear restriction fragment length polymorphism (RFLP). Mol Biol Evol 9:872–892
Gentzbittel L, Zhang Y-X, Vear F, Griveau Y, Nicolas P (1994) RFLP studies of genetic relationships among inbred lines of cultivated sunflower, Helianthus annuus L.: evidence for distinct restorer and maintainer germplasm pools. Theor Appl Genet 89:419–425
Gentzbittel L, Vear F, Zhang Y-X, Bervillé A, Nicolas P (1995) Development of a consensus linkage RFLP map of cultivated sunflower (Helianthus annuus L.) Theor Appl Genet (in press)
Gulya T J, Miller J F, Viranyi F, Sackston W E (1991a) Proposed internationally standardized methods for race identification of Plasmopara halstedii. Helia 14:11–20
Gulya T J, Sackston W E, Viranyi F, Masirevic S, Rashid K Y (1991b) New races of the sunflower downy mildew pathogen (Plasmopara halstedii) in Europe and North and South America. J Phytopathol 132:303–311
Holloway JL, Knapp SJ (1993) Gmendel 3.0 Users Guide. Department of Crop and Soil Science, Oregon State University, USA.
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to the Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
Melchinger AE (1990) Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed 104:1–19
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Mindrinos M, Katagiri F, Yu G-L, Ausubel FM (1994) The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell 78: 1089–1099
Miller JF, Gulya TJ (1991) Inheritance of resistance to race 4 of downy mildew derived from interspecific crosses in sunflower. Crop Sci 31:40–43
Mouzeyar S, Philippon J, Vear F, Tourvieille D, (1992) Genetical studies of resistance to downy mildew (Plasmopara helianthi Novot). In: ISA, CETIOM Paris (eds) Sunflowers. Proc 13th Int Sunfl Conf, Pisa Italy, pp 1162–1167
Mouzeyar S, Tourvieille De Labrouhe D, Vear F (1993) Histopathological studies of resistance of sunflower (Helianthus annuus L.) to downy mildew (Plasmopara halstedii). J Phytopathol 139:289–297
Mouzeyar S, Tourvieille De Labrouhe D, Vear F (1994) Effect of host-race combination on resistance of sunflower (Helianthus annuus L.) to downy mildew (Plasmopara halstedii). J Phytopathol 141:249–258
Quillet MC, Vear F, Branlard G (1992) The use of isozyme polymorphism for identification of sunflower (Helianthus annuus) inbred lines. J Genet Breed 46:295–304
Sackston WE (1981) Downy mildew of sunflower. In: Spencer DM (ed) The downy mildews. Academic Press, London New York, pp 545–575
Sackston WE (1992) On a treadmill: breeding sunflowers for resistance to disease. Annu Rev Phytopathol 30:529–551
Sackston WE, Gulya TJ, Miller JF (1990) A proposed international system for designation of races of Plasmopara halstedii. Plant Dis 74:721–723
Teulat B, Zhang YX, Nicolas P (1994) Characteristics of random amplified DNA markers discriminating Helianthus annuus inbred lines. Agronomie 14:497–502
Vear F (1974) Studies on resistance to downy mildew in sunflowers. In: ISA, CETIOM Paris (eds) Proc 6th Int Sunflower Conf, Bucharest Romania, pp 297–302
Vear F (1978) Reaction de certains génotypes de tournesol résistants au mildiou (Plasmopara helianthi) au test de résistance sur plante. Ann Amélior Plant 28:327–332
Vranceanu V (1970) Advances in sunflower breeding in Romania. In: ISA, CETIOM Paris (eds) Proc 4th Int Sunflower Conf, Memphis Tennessee USA pp 136–148
Vranceanu V, Pirvu N, Stoenescu FM (1981) New sunflower downy mildew resistance genes and their management. Helia 4:23–27
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Young N, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify markers tightly linked to the TM-2a gene in tomato. Genetics 120:579–585.
Zhang Q, Shen BZ, Dai XK, Mei MH, Saghai Maroof MA, Li ZB (1994) Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice. Proc Natl Acad Sci USA 91:8675–8679
Zimmer DE, Kinmann ML (1972) Downy mildew resistance in cultivated sunflower and its inheritance. Crop Sci 12:749–751
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
Rights and permissions
About this article
Cite this article
Mouzeyar, S., Roeckel-Drevet, P., Gentzbittel, L. et al. RFLP and RAPD mapping of the sunflower Pl1 locus for resistance to Plasmopara halstedii race 1. Theoret. Appl. Genetics 91, 733–737 (1995). https://doi.org/10.1007/BF00220951
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220951