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Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew

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Abstract

Major gene resistance to sunflower downy mildew (Plasmopara halstedii) races 304 and 314 was found to segregate independently from the resistance to races 334, 307 and 304 determined by the gene Pl2, already positioned on Linkage Group (LG) 8 of sunflower molecular maps. Using a consensus SSR–SNP map constructed from the INEDI RIL population and a new RIL population FU × PAZ2, the positions of Pl2 and Pl5 were confirmed and the new gene, denoted Pl21, was mapped on LG13, at 8 cM from Pl5. The two RIL populations were observed for their quantitative resistance to downy mildew in the field and both indicated the existence of a QTL on LG8 at 20–40 cM from the major resistance gene cluster. In addition, for the INEDI population, a strong QTL on LG10, reported previously, was confirmed and a third QTL was mapped on LG7. A growth chamber test methodology, significantly correlated with field results, also revealed the major QTL on LG10, explaining 65 % of variability. This QTL mapped in the same area as a gene involved in stomatal opening and root growth, which may be suggested as a possible candidate to explain the control of this character. These results indicate that it should be possible to combine major genes and other resistance mechanisms, a strategy that could help to improve durability of sunflower resistance to downy mildew.

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Acknowledgments

We would like to thank S. Roche, M-C. Boniface, N. Blanchet, D. Jolivot, P. Walser and the INRA farm, greenhouse and growth chamber teams at Clermont-Ferrand and Toulouse for their expert technical help. PROMOSOL provided financial support for this research. SNP genotyping data were obtained in collaboration with the companies BIOGEMMA, SYNGENTA SEEDS, SOLTIS and RAGT, during the “SUNYFUEL” project, financially supported by the French National Research Agency, and the OLEOSOL project, with the financial support from the Midi Pyrénées Region, the European Fund for Regional Development (EFRD), and the French Fund for Competitiveness Clusters (FUI).

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Authors and Affiliations

  1. Laboratoire des Interactions Plantes-Microorganismes (LIPM), INRA, UMR441, 31326, Castanet-Tolosan, France

    Patrick Vincourt, Falah As-sadi, Amandine Bordat, Nicolas B. Langlade, Jerome Gouzy, Nicolas Pouilly, Yannick Lippi & Laurence Godiard

  2. Laboratoire des Interactions Plantes-Microorganismes (LIPM), CNRS, UMR2594, 31326, Castanet-Tolosan, France

    Patrick Vincourt, Falah As-sadi, Amandine Bordat, Nicolas B. Langlade, Jerome Gouzy, Nicolas Pouilly, Yannick Lippi & Laurence Godiard

  3. INRA, UMR 1095, Domaine de Crouelle, 234, Ave du Brezet, 63000, Clermont-Ferrand, France

    Frédéric Serre, Denis Tourvieille de Labrouhe & Felicity Vear

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  1. Patrick Vincourt
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Correspondence to Patrick Vincourt or Felicity Vear.

Additional information

Communicated by H. H. Geiger.

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122_2012_1882_MOESM1_ESM.xlsx

Supplementary file 1: Helianthus annuus candidate gene sequences chosen for SNP identification and mapping (CG_code, HU_cluster (http://www.heliagene.org) or HP_cluster (http://www.heliagene.org/HP), Arabidopsis thaliana_homologue, FWD_primer_name, FWD_primer_sequence, REV_primer_name, REV_primer_sequence) (XLSX 94 kb)

Supplementary file 2: List of 48 sunflower lines used to detect SNP polymorphism on candidate genes (XLSX 14 kb)

Supplementary file 3: Consensus map built on INEDI and FU × PAZ2 RIL populations (XLSX 1573 kb)

Supplementary file 4: Partial sequence of CG0103 (fasta format) (TXT 1 kb)

Supplementary file 5: Partial sequence of CG0449 (fasta format) (TXT 0 kb)

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Vincourt, P., As-sadi, F., Bordat, A. et al. Consensus mapping of major resistance genes and independent QTL for quantitative resistance to sunflower downy mildew. Theor Appl Genet 125, 909–920 (2012). https://doi.org/10.1007/s00122-012-1882-y

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