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Genetics and mapping of the R 11 gene conferring resistance to recently emerged rust races, tightly linked to male fertility restoration, in sunflower (Helianthus annuus L.)

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Abstract

Sunflower oil is one of the major sources of edible oil. As the second largest hybrid crop in the world, hybrid sunflowers are developed by using the PET1 cytoplasmic male sterility system that contributes to a 20 % yield advantage over the open-pollinated varieties. However, sunflower production in North America has recently been threatened by the evolution of new virulent pathotypes of sunflower rust caused by the fungus Puccinia helianthi Schwein. Rf ANN-1742, an ‘HA 89’ backcross restorer line derived from wild annual sunflower (Helianthus annuus L.), was identified as resistant to the newly emerged rust races. The aim of this study was to elucidate the inheritance of rust resistance and male fertility restoration and identify the chromosome location of the underlying genes in Rf ANN-1742. Chi-squared analysis of the segregation of rust response and male fertility in F2 and F3 populations revealed that both traits are controlled by single dominant genes, and that the rust resistance gene is closely linked to the restorer gene in the coupling phase. The two genes were designated as R 11 and Rf5, respectively. A set of 723 mapped SSR markers of sunflower was used to screen the polymorphism between HA 89 and the resistant plant. Bulked segregant analysis subsequently located R 11 on linkage group (LG) 13 of sunflower. Based on the SSR analyses of 192 F2 individuals, R 11 and Rf5 both mapped to the lower end of LG13 at a genetic distance of 1.6 cM, and shared a common marker, ORS728, which was mapped 1.3 cM proximal to Rf5 and 0.3 cM distal to R 11 (Rf5/ORS728/R 11 ). Two additional SSRs were linked to Rf5 and R 11 : ORS995 was 4.5 cM distal to Rf5 and ORS45 was 1.0 cM proximal to R 11 . The advantage of such an introduced alien segment harboring two genes is its large phenotypic effect and simple inheritance, thereby facilitating their rapid deployment in sunflower breeding programs. Suppressed recombination was observed in LGs 2, 9, and 11 as it was evident that no recombination occurred in the introgressed regions of LGs 2, 9, and 11 detected by 5, 9, and 22 SSR markers, respectively. R 11 is genetically independent from the rust R-genes R 1 , R 2 , and R 5 , but may be closely linked to the rust R-gene R adv derived from wild Helianthus argophyllus, forming a large rust R-gene cluster of R adv /R 11 /R 4 in the lower end of LG13. The relationship of Rf5 with Rf1 is discussed based on the marker association analysis.

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Acknowledgments

We thank Drs. Xiwen Cai and Zengcui Zhang for critical review of the manuscript, and Angelia Hogness and Hannah Worral for technical assistance. This project was supported by the USDA-ARS CRIS Project No. 5442-21000-034-00D.

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  1. Northern Crop Science Laboratory, USDA, Agricultural Research Service, 1605 Albrecht Blvd N, Fargo, ND, 58102-2765, USA

    L. L. Qi, G. J. Seiler, B. A. Vick & T. J. Gulya

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  1. L. L. Qi
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  2. G. J. Seiler
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Correspondence to L. L. Qi.

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Communicated by A. Bervillé.

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Qi, L.L., Seiler, G.J., Vick, B.A. et al. Genetics and mapping of the R 11 gene conferring resistance to recently emerged rust races, tightly linked to male fertility restoration, in sunflower (Helianthus annuus L.). Theor Appl Genet 125, 921–932 (2012). https://doi.org/10.1007/s00122-012-1883-x

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