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DNA markers linked to the R 2 rust resistance gene in sunflower (Helianthus annuus L.) facilitate anticipatory breeding for this disease variant

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Abstract

Pre-emptive breeding for host disease resistance is an effective strategy for combating and managing devastating incursions of plant pathogens. Comprehensive, long-term studies have revealed that virulence to the R 2 sunflower (Helianthus annuus L.) rust resistance gene in the line MC29 does not exist in the Australian rust (Puccinia helianthi) population. We report in this study the identification of molecular markers linked to this gene. The three simple sequence repeat (SSR) markers ORS795, ORS882, and ORS938 were linked in coupling to the gene, while the SSR marker ORS333 was linked in repulsion. Reliable selection for homozygous-resistant individuals was efficient when the three markers, ORS795, ORS882, and ORS333, were used in combination. Phenotyping for this resistance gene is not possible in Australia without introducing a quarantinable race of the pathogen. Therefore, the availability of reliable and heritable DNA-based markers will enable the efficient deployment of this gene, permitting a more effective strategy for generating sustainable commercial cultivars containing this rust resistance gene.

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Acknowledgments

The authors wish to acknowledge the financial support of the Grains Research and Development Corporation, the former Cooperative Research Centre for Tropical Plant Pathology, and the Queensland Government.

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Authors and Affiliations

  1. Former Cooperative Research Centre for Tropical Plant Protection, Level 5, John Hines Building, The University of Queensland, Brisbane, Queensland, 4072, Australia

    W. R. Lawson, T. Shatte, L. Smith, G. A. Kong & J. K. Kochman

  2. Agri-Science Queensland, Department of Employment, Economic Development and Innovation, Hermitage Research Station, 604 Yangan Road, Warwick, Queensland, 4370, Australia

    W. R. Lawson & T. Shatte

  3. Agri-Science Queensland, Department of Employment, Economic Development and Innovation, Tor St, PO Box 102, Toowoomba, Queensland, 4350, Australia

    L. Smith & G. A. Kong

  4. Former Queensland Department of Primary Industries and Fisheries, Tor St, PO Box 102, Toowoomba, Queensland, 4350, Australia

    J. K. Kochman

  5. USDA-ARS, Northern Crop Science Laboratory, Fargo, ND, 58105, USA

    C. C. Jan

Authors
  1. W. R. Lawson
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  2. C. C. Jan
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  3. T. Shatte
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  4. L. Smith
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  5. G. A. Kong
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  6. J. K. Kochman
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Corresponding author

Correspondence to W. R. Lawson.

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© State of Queensland, Department of Employment, Economic Development and Innovation, 2010.

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Lawson, W.R., Jan, C.C., Shatte, T. et al. DNA markers linked to the R 2 rust resistance gene in sunflower (Helianthus annuus L.) facilitate anticipatory breeding for this disease variant. Mol Breeding 28, 569–576 (2011). https://doi.org/10.1007/s11032-010-9506-1

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  • DOI: https://doi.org/10.1007/s11032-010-9506-1

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