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Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2

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Abstract 

The brown planthopper (BPH), Nilaparvata lugens Stål, is a significant insect pest of rice (Oryza sa-tiva L.). bph2 is one of the 12 major BPH resistance genes so far identified in several indica cultivars and two wild relatives. We have constructed a high-resolution linkage map as a foundation for map-based cloning of the bph2 locus. An advanced mapping population derived from a cross of ’Tsukushibare’ (a susceptible japonica cultivar) with ’Norin-PL4’ (an authentic bph2-introgression line) was used. Segregation analysis by the mass seedling test showed that bph2 behaved as a single dominant gene. Through bulked segregant analysis and linkage analysis, bph2 was located within a 3.2-cM region containing eight AFLP markers. One marker (KAM4) showed complete co-segregation with bph2, and bph2 was mapped within a 1.0-cM region delimited by KAM3 and KAM5, two flanking markers. KAM4 was converted into a PCR-based sequence-tagged-site (STS) marker and its co-segregation with bph2 was validated.

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Authors and Affiliations

  1. Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Division of Life Science, Graduate School of Science and Technology, Kobe University, 1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan e-mail: nakamura@kobe-u.ac.jp Fax: +81-78-803-5858, Present address: K. Murata, Rice Genome Research Program, STAFF-Institute, 446-1 Ippaizuka, Kamiyokoba, Tsukuba 305-0854, Japan, , , , , , JP

    H. Murai, Z. Hashimoto, P. N. Sharma, T. Shimizu, K. Murata, S. Takumi, N. Mori & C. Nakamura

  2. National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba 305-8602, Japan, , , , , , JP

    S. Kawasaki

Authors
  1. H. Murai
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  2. Z. Hashimoto
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  3. P. N. Sharma
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  4. T. Shimizu
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  5. K. Murata
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  6. S. Takumi
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  7. N. Mori
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  8. S. Kawasaki
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  9. C. Nakamura
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Received: 26 June 2000 / Accepted: 26 January 2001

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Murai, H., Hashimoto, Z., Sharma, P. et al. Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph2. Theor Appl Genet 103, 526–532 (2001). https://doi.org/10.1007/s001220100598

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  • DOI: https://doi.org/10.1007/s001220100598

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