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Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat

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Abstract

Stripe rust, caused by Puccinia striiformis West. f.sp. tritici, is one of the most damaging diseases of wheat worldwide. Forty genes for stripe rust resistance have been catalogued so far, but the majority of them are not effective against emerging pathotypes. Triticum monococcum and T. boeoticum have excellent levels of resistance to rusts, but so far, no stripe rust resistance gene has been identified or transferred from these species. A set of 121 RILs generated from a cross involving T. monococcum (acc. pau14087) and T. boeoticum (acc. pau5088) was screened for 3 years against a mixture of pathotypes under field conditions. The parental accessions were susceptible to all the prevalent pathotypes at the seedling stage, but resistant at the adult plant stage. Genetic analysis of the RIL population revealed the presence of two genes for stripe rust resistance, with one gene each being contributed by each of the parental lines. A linkage map with 169 SSR and RFLP loci generated from a set of 93 RILs was used for mapping these resistance genes. Based on phenotypic data for 3 years and the pooled data, two QTLs, one each in T. monococcum acc. pau14087 and T. boeoticum acc. pau5088, were detected for resistance in the RIL population. The QTL in T. monococcum mapped on chromosome 2A in a 3.6 cM interval between Xwmc407 and Xwmc170, whereas the QTL from T. boeoticum mapped on 5A in 8.9 cM interval between Xbarc151 and Xcfd12 and these were designated as QYrtm.pau-2A and QYrtb.pau-5A, respectively. Based on field data for 3 years, their R 2 values were 14 and 24%, respectively. T. monococcum acc. pau14087 and three resistant RILs were crossed to hexaploid wheat cvs WL711 and PBW343, using T. durum as a bridging species with the objective of transferring these genes into hexaploid wheat. The B genome of T. durum suppressed resistance in the F1 plants, but with subsequent backcrossing one resistance gene could be transferred from one of the RILs to the hexaploid wheat background. This gene was derived from T. boeoticum acc. pau5088 as indicated by co-introgression of T. boeoticum sequences linked to stripe rust resistance QTL, QYrtb.pau-5A. Homozygous resistant progenies with 40–42 chromosomes have been identified.

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Acknowledgments

This work was carried under Indo-Swiss collaboration in Biotechnology. The financial support provided by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, Swiss Agency for Development and Cooperation (SDC; to KS and BK) and the Swiss National Science Foundation (3100-105620 (BK) is gratefully acknowledged. We are thankful to the staff of the wheat breeding section of the department for the rust inoculum.

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  1. H. S. Dhaliwal

    Present address: Indian Institute of Technology, Roorkee, Uttarakhand, India

Authors and Affiliations

  1. Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India

    Parveen Chhuneja, Satinder Kaur, Tosh Garg, Meenu Ghai, Simarjit Kaur, N. S. Bains, R. K. Goel, H. S. Dhaliwal & Kuldeep Singh

  2. Directorate of Wheat Research, Regional Station, Flowerdale, Shimla, India

    M. Prashar

  3. Institute of Plant Biology, University of Zurich, Zurich, Switzerland

    Beat Keller

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  1. Parveen Chhuneja
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Correspondence to Kuldeep Singh.

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Communicated by B. Friebe.

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Chhuneja, P., Kaur, S., Garg, T. et al. Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theor Appl Genet 116, 313–324 (2008). https://doi.org/10.1007/s00122-007-0668-0

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