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Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii

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Abstract

Hexaploid wheat (Triticum aestivum L.) originated about 8,000 years ago from the hybridization of tetraploid wheat with diploid Aegilops tauschii Coss. containing the D-genome. Thus, the bread wheat D-genome is evolutionary young and shows a low degree of polymorphism in the bread wheat gene pool. To increase marker density around the durable leaf rust resistance gene Lr34 located on chromosome 7DS, we used molecular information from the orthologous region in rice. Wheat expressed sequence tags (wESTs) were identified by homology with the rice genes in the interval of interest, but were monomorphic in the ‘Arina’ × ‘Forno’ mapping population. To derive new polymorphic markers, bacterial artificial chromosome (BAC) clones representing a total physical size of ∼1 Mb and belonging to four contigs were isolated from Ae. tauschii by hybridization screening with wheat ESTs. Several BAC clones were low-pass sequenced, resulting in a total of ∼560 kb of sequence. Ten microsatellite sequences were found, and three of them were polymorphic in our population and were genetically mapped close to Lr34. Comparative analysis of marker order revealed a large inversion between the rice genome and the wheat D-genome. The SWM10 microsatellite is closely linked to Lr34 and has the same allele in the three independent sources of Lr34: ‘Frontana’, ‘Chinese Spring’, and ‘Forno’, as well in most of the genotypes containing Lr34. Therefore, SWM10 is a highly useful marker to assist selection for Lr34 in breeding programs worldwide.

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Acknowledgments

We would like to thank Dr. Thomas Wicker and Dr. Edith Schlagenhauf for their bioinformatical support and Beatrice Senger, Theres Klimm and Urs Ochsner for their technical assistance. We are very thankful to Dr. Jan Dvorak and Dr. Alina Akhunova (University of California, Davis, CA, USA) for providing BAC clones and filters of the Ae. tauschii BAC library, and to Dr. Sylvie Cloutier and Dr. Andrzej Walichnowski (Cereal Research Center, Winnipeg, Canada) for providing the BAC pools of the ‘Glenlea’ library as well as wheat ESTs. For providing ESTs we also thank Dr. Olin Anderson and Dr. Curt Crossman (USDA-ARS, Albany, CA, USA), Dr. Yasunari Ogihara (Yokohama City University, Japan), Dr. Kazuhiro Sato (Okayama University, Japan), Dr. Mark Sorrells (Cornell University, Ithaca, NY, USA), Dr. Jens Stougaard (Aarhus University, Denmark), Dr. Patrick Gulick (Concordia University, Montreal, Canada), Dr. Nils Stein (IPK Gatersleben, Germany), and Dr. Robert Waugh (SCRI, Dundee, Scotland, UK). We thank Dr. Marion Röder (IPK Gatersleben, Germany) as well as TraitGenetics Inc (Gatersleben, Germany) for providing unpublished primer sequences. We are grateful to Dr. Jon Raupp (KSU, WGRC, Manhattan, KS, USA) for providing plant material. Finally we would like to thank Dr. Nabila Yahiaoui and Dr. Jan Dvorak for critical reading of the manuscript. This work was supported by the Swiss National Science Foundation (SNF grant 3100A0-105620) and by the Indo–Swiss Collaboration in Biotechnology (ISCB, project WN7). The authors declare that the experiments conducted for this publication comply with the current laws of Switzerland. Eligio Bossolini and Simon G. Krattinger contributed equally to the work.

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  1. Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008, Zürich, Switzerland

    Eligio Bossolini, Simon G. Krattinger & Beat Keller

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  1. Eligio Bossolini
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Correspondence to Beat Keller.

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Communicated by A. Graner.

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Bossolini, E., Krattinger, S.G. & Keller, B. Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii . Theor Appl Genet 113, 1049–1062 (2006). https://doi.org/10.1007/s00122-006-0364-5

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