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Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat

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Abstract

Leaf rust and stripe rust are important foliar diseases of wheat worldwide. Leaf rust and stripe rust resistant introgression lines were developed by induced homoeologous chromosome pairing between wheat chromosome 5D and 5Mg of Aegilops geniculata (UgMg). Characterization of rust resistant BC2F5 and BC3F6 homozygous progenies using genomic in situ hybridization with Aegilops comosa (M) DNA as probe identified three different types of introgressions; two cytologically visible and one invisible (termed cryptic alien introgression). All three types of introgression lines showed similar and complete resistance to the most prevalent pathotypes of leaf rust and stripe rust in Kansas (USA) and Punjab (India). Diagnostic polymorphisms between the alien segment and recipient parent were identified using physically mapped RFLP probes. Molecular mapping revealed that cryptic alien introgression conferring resistance to leaf rust and stripe rust comprised less than 5% of the 5DS arm and was designated T5DL·5DS-5MgS(0.95). Genetic mapping with an F2 population of Wichita × T5DL·5DS-5MgS(0.95) demonstrated the monogenic and dominant inheritance of resistance to both diseases. Two diagnostic RFLP markers, previously mapped on chromosome arm 5DS, co-segregated with the rust resistance in the F2 population. The unique map location of the resistant introgression on chromosome T5DL·5DS-5MgS(0.95) suggested that the leaf rust and stripe rust resistance genes were new and were designated Lr57 and Yr40. This is the first documentation of a successful transfer and characterization of cryptic alien introgression from Ae. geniculata conferring resistance to both leaf rust and stripe rust in wheat.

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Acknowledgment

We thank Robert McIntosh for providing the new gene designations for the rust resistant genes reported in the present study. We extend a special note of thanks to Duane Wilson and John Raupp for their excellent technical assistance. We also thank Bernd Friebe and Li Huang for their helpful discussions and Xianming Chen for race determination of P. striiformis. This paper is contribution number 07–40-J from the Kansas Agricultural Experimentation Station, Kansas State University, Manhattan, Kansas.

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Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA

    Vasu Kuraparthy & Bikram S. Gill

  2. Department of Plant Breeding, Genetics & Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, Punjab, India

    Parveen Chhuneja & Satinder Kaur

  3. Department of Biotechnology, Indian Institute of Technology, Roorkee, 247667, Uttaranchal, India

    Harcharan S. Dhaliwal

  4. Plant Science and Entomology Research Unit, USDA-ARS, Kansas State University, Manhattan, KS, 66506-5502, USA

    Robert L. Bowden

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  1. Vasu Kuraparthy
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  2. Parveen Chhuneja
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  3. Harcharan S. Dhaliwal
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Correspondence to Bikram S. Gill.

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Communicated by P. Langridge.

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Kuraparthy, V., Chhuneja, P., Dhaliwal, H.S. et al. Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet 114, 1379–1389 (2007). https://doi.org/10.1007/s00122-007-0524-2

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