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The Use of Omics Technologies, Random Mutagenesis, and Genetic Transformation Techniques to Improve Algae for Biodiesel Industry

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Technological Advancement in Algal Biofuels Production

Part of the book series: Clean Energy Production Technologies ((CEPT))

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Abstract

Biodiesel as a transportation fuel has gained interest because of fossil fuel depletion and global warming. Algae, with the ability to grow fast in a variety of conditions, are a sustainable choice for biodiesel production. The most critical challenge is insufficient lipid yield in algal sources and associated high biorefinery costs. It is well-known that some algal strains accumulate relatively high lipid under environmental or nutritional stress that hinder algal growth. Genomic, transcriptomic, and proteomic analyses of stressed algal strains help identify and characterize essential molecules that induce lipid accumulation. Random mutagenesis, in addition to the development of algal strains with high lipid content for the biodiesel industry, provides clues for further genetic engineering attempts if combined with omics technologies. Another powerful genetic engineering tool to improve algae growth, lipid content, and stress tolerance is genetic transformation. In the present chapter, first, the commonly used omics methods and the use of omics data to improve algae species with potential in the biodiesel industry are summarized. In addition, random mutagenesis and its applications in strain improvement for the biodiesel industry are examined. Finally, genetic transformation methods and vector construction strategies are reviewed as well as up-to-date reports on the progress in algal transformation for biodiesel production.

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  1. Department of Molecular Biology and Genetics, Science Faculty, University of Ondokuz Mayıs, Samsun, Turkey

    Ali Osman Adiguzel

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  1. Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, Varanasi, India

    Neha Srivastava

  2. Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, Varanasi, India

    P. K. Mishra

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Adiguzel, A.O. (2023). The Use of Omics Technologies, Random Mutagenesis, and Genetic Transformation Techniques to Improve Algae for Biodiesel Industry. In: Srivastava, N., Mishra, P.K. (eds) Technological Advancement in Algal Biofuels Production. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-19-6806-8_2

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