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Transient expression of the GUS gene in a unicellular marine green alga,Chlorella sp.MACC/C95, via electroporation

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Abstract

A transient expression system for a unicellular marine green alga,Chlorella sp.MACC/C95, was developed using a reporter GUS gene coded for by plasmid pBI121. The results demonstrated a high transformation efficiency could be achieved by using electroporation to deliver DNA into intact cells and the CaMV35S promoter to drive the foreign gene expression inChlorella sp.MACC/C95. The use of a carrier DNA coupled with osmosis treatment improved the transformation efficiency, while linearization of the plasmid had minor effects. Investigation of the effects of DNA concentration and growth phases ofChlorella sp.MACC/C95 on transformation efficiency indicated that the highest level of transient expression was observed when 6 μg mL−1 of plasmid DNA and cells 2–6 days old were used.

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Authors and Affiliations

  1. School of Ocean, Yantai University, 264005, Yantai, P. R. China

    Changhai Wang

  2. Marine Biochemical Engineering Lab, Dalian University of Technology, 116024, Dalian, P. R. China

    Changhai Wang, Yiyun Wang, Qiao Su & Xiaorong Gao

Authors
  1. Changhai Wang
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  2. Yiyun Wang
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  3. Qiao Su
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  4. Xiaorong Gao
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Correspondence to Changhai Wang.

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Wang, C., Wang, Y., Su, Q. et al. Transient expression of the GUS gene in a unicellular marine green alga,Chlorella sp.MACC/C95, via electroporation. Biotechnol. Bioprocess Eng. 12, 180–183 (2007). https://doi.org/10.1007/BF03028646

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  • DOI: https://doi.org/10.1007/BF03028646

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