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Filamentous Fungi as Hosts for Heterologous Production of Proteins and Secondary Metabolites in the Post-Genomic Era

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Genetics and Biotechnology

Part of the book series: The Mycota ((MYCOTA,volume 2))

Abstract

Filamentous fungi are frequently used as hosts for heterologous production of industrial enzymes and bioactive secondary metabolites. This role will likely increase in the post-genomic era as a multitude of fungal genome sequences are mined for new valuable products. The trend for constructing fungal cell factories is to use synthetic biology approaches. In simplicity, this includes puzzling available bio-blocks together using seamless DNA fusions to generate vectors, gene-expression cassettes, and protein fusions. Moreover, it also sets the stage to expand natural enzymatic functions and chemistry by combining functional domains into synthetic proteins with novel enzymatic properties or by combining enzymes from different organisms in a common host to establish new biosynthetic pathways. In this chapter, we describe the components of a modern synthetic biology toolbox and how they have been used to construct and optimize fungal cell factories for heterologous production of natural and synthetic enzymes and secondary metabolites.

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Acknowledgments

We are grateful to Michael Lynge Nielsen for carefully reading and editing this book chapter. This work has been supported by Innovation Fund Denmark, grant no.: 6150-00031B.

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Authors and Affiliations

  1. Technical University of Denmark, Kgs. Lyngby, Denmark

    Jakob K. H. Rendsvig, Malgorzata E. Futyma, Zofia D. Jarczynska & Uffe H. Mortensen

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  1. Jakob K. H. Rendsvig
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  2. Malgorzata E. Futyma
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  3. Zofia D. Jarczynska
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  4. Uffe H. Mortensen
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Correspondence to Uffe H. Mortensen .

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  1. TUM School of Life Sciences Weihenstephan, Holzforschung München – Fungal Biotechnology in Wood Science, Technische Universität München, Freising, Germany

    J. Philipp Benz

  2. Institut für Mikrobiologie, Department Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany

    Kerstin Schipper

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Rendsvig, J.K.H., Futyma, M.E., Jarczynska, Z.D., Mortensen, U.H. (2020). Filamentous Fungi as Hosts for Heterologous Production of Proteins and Secondary Metabolites in the Post-Genomic Era. In: Benz, J.P., Schipper, K. (eds) Genetics and Biotechnology. The Mycota, vol 2. Springer, Cham. https://doi.org/10.1007/978-3-030-49924-2_10

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