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Development of an expression vector for Metarhizium anisopliae based on the tef-1α homologous promoter

  • Applied Genetics and Molecular Biotechnology
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Abstract

The high-conserved translation elongation factor 1 α (tef-1α) gene from the enthomopathogenic fungus Metarhizium anisopliae was characterized to select the promoter region. A 640-bp DNA fragment upstream to the start codon was employed to drive the expression of the reporter protein sGFP or a dominant selectable marker, the gene bar (resistance to ammonium glufosinate). Transformants carrying this homologous promoter system showed no difference in virulence bioassays against the cattle tick Boophilus microplus comparing to the M. anisopliae wild-type strain. Moreover, GFP fluorescence was detected during tick infection bioassay.

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Acknowledgements

This work was supported by CNPq and CAPES. We thank Dr. J.R. Martins from IPVDF Institute for kindly providing the B. microplus engorged females. We thank the two anonymous referees for useful comments.

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Authors and Affiliations

  1. Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil

    Luciano Nakazato, Valéria Dutra, Leonardo Broetto, Charley Christian Staats, Marilene Henning Vainstein & Augusto Schrank

  2. Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil

    Augusto Schrank

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  1. Luciano Nakazato
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  2. Valéria Dutra
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  4. Charley Christian Staats
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  5. Marilene Henning Vainstein
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  6. Augusto Schrank
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Correspondence to Augusto Schrank.

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Nakazato, L., Dutra, V., Broetto, L. et al. Development of an expression vector for Metarhizium anisopliae based on the tef-1α homologous promoter. Appl Microbiol Biotechnol 72, 521–528 (2006). https://doi.org/10.1007/s00253-005-0292-3

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  • DOI: https://doi.org/10.1007/s00253-005-0292-3

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